Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha

Autores
Imperiale, Julieta Celeste; Schlachet, Inbar; Lewicki, Marianela; Sosnik, Alejandro Dario; Biglione, Mirna Marcela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract: Interferon alpha (IFN) is a protein drug used to treat viral infections and cancer diseases. Dueto its poor stability in the gastrointestinal tract, only parenteral administration ensures bioavailability,which is associated with severe side eects. We hypothesized that the nanoencapsulation ofIFN within nanoparticles of the mucoadhesive polysaccharide chitosan would improve theoral bioavailability of this drug. In this work, we produced IFN-loaded chitosan nanoparticlesby the ionotropic gelation method. Their hydrodynamic diameter, polydispersity index andconcentration were characterized by dynamic light scattering and nanoparticle tracking analysis.After confirming their good cell compatibility in Caco-2 and WISH cells, the permeability ofunmodified and poly(ethylene glycol) (PEG)-modified (PEGylated) nanoparticles was measured inmonoculture (Caco-2) and co-culture (Caco-2/HT29-MTX) cell monolayers. Results indicated thatthe nanoparticles cross the intestinal epithelium mainly by the paracellular route. Finally, the studyof the oral pharmacokinetics of nanoencapsulated IFN in BalbC mice revealed two maxima andarea-under-the-curve of 56.9 pg*h/mL.
Fil: Imperiale, Julieta Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina
Fil: Schlachet, Inbar. Technion - Israel Institute of Technology; Israel
Fil: Lewicki, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel
Fil: Biglione, Mirna Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
Materia
IFNΑ
IN VITRO INTESTINAL PERMEABILITY
ORAL PHARMACOKINETICS
ORAL PROTEIN DELIVERY
POLYMERIC NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/122998
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/122998
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon AlphaImperiale, Julieta CelesteSchlachet, InbarLewicki, MarianelaSosnik, Alejandro DarioBiglione, Mirna MarcelaIFNΑIN VITRO INTESTINAL PERMEABILITYORAL PHARMACOKINETICSORAL PROTEIN DELIVERYPOLYMERIC NANOPARTICLEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Abstract: Interferon alpha (IFN) is a protein drug used to treat viral infections and cancer diseases. Dueto its poor stability in the gastrointestinal tract, only parenteral administration ensures bioavailability,which is associated with severe side eects. We hypothesized that the nanoencapsulation ofIFN within nanoparticles of the mucoadhesive polysaccharide chitosan would improve theoral bioavailability of this drug. In this work, we produced IFN-loaded chitosan nanoparticlesby the ionotropic gelation method. Their hydrodynamic diameter, polydispersity index andconcentration were characterized by dynamic light scattering and nanoparticle tracking analysis.After confirming their good cell compatibility in Caco-2 and WISH cells, the permeability ofunmodified and poly(ethylene glycol) (PEG)-modified (PEGylated) nanoparticles was measured inmonoculture (Caco-2) and co-culture (Caco-2/HT29-MTX) cell monolayers. Results indicated thatthe nanoparticles cross the intestinal epithelium mainly by the paracellular route. Finally, the studyof the oral pharmacokinetics of nanoencapsulated IFN in BalbC mice revealed two maxima andarea-under-the-curve of 56.9 pg*h/mL.Fil: Imperiale, Julieta Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Schlachet, Inbar. Technion - Israel Institute of Technology; IsraelFil: Lewicki, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; IsraelFil: Biglione, Mirna Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; ArgentinaMDPI AG2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/122998Imperiale, Julieta Celeste; Schlachet, Inbar; Lewicki, Marianela; Sosnik, Alejandro Dario; Biglione, Mirna Marcela; Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha; MDPI AG; Polymers; 11; 11; 11-2019; 1-132073-4360CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/11/11/1862info:eu-repo/semantics/altIdentifier/doi/10.3390/polym11111862info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:59:44Zoai:ri.conicet.gov.ar:11336/122998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:59:44.546CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
title Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
spellingShingle Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
Imperiale, Julieta Celeste
IFNΑ
IN VITRO INTESTINAL PERMEABILITY
ORAL PHARMACOKINETICS
ORAL PROTEIN DELIVERY
POLYMERIC NANOPARTICLES
title_short Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
title_full Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
title_fullStr Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
title_full_unstemmed Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
title_sort Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha
dc.creator.none.fl_str_mv Imperiale, Julieta Celeste
Schlachet, Inbar
Lewicki, Marianela
Sosnik, Alejandro Dario
Biglione, Mirna Marcela
author Imperiale, Julieta Celeste
author_facet Imperiale, Julieta Celeste
Schlachet, Inbar
Lewicki, Marianela
Sosnik, Alejandro Dario
Biglione, Mirna Marcela
author_role author
author2 Schlachet, Inbar
Lewicki, Marianela
Sosnik, Alejandro Dario
Biglione, Mirna Marcela
author2_role author
author
author
author
dc.subject.none.fl_str_mv IFNΑ
IN VITRO INTESTINAL PERMEABILITY
ORAL PHARMACOKINETICS
ORAL PROTEIN DELIVERY
POLYMERIC NANOPARTICLES
topic IFNΑ
IN VITRO INTESTINAL PERMEABILITY
ORAL PHARMACOKINETICS
ORAL PROTEIN DELIVERY
POLYMERIC NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Abstract: Interferon alpha (IFN) is a protein drug used to treat viral infections and cancer diseases. Dueto its poor stability in the gastrointestinal tract, only parenteral administration ensures bioavailability,which is associated with severe side eects. We hypothesized that the nanoencapsulation ofIFN within nanoparticles of the mucoadhesive polysaccharide chitosan would improve theoral bioavailability of this drug. In this work, we produced IFN-loaded chitosan nanoparticlesby the ionotropic gelation method. Their hydrodynamic diameter, polydispersity index andconcentration were characterized by dynamic light scattering and nanoparticle tracking analysis.After confirming their good cell compatibility in Caco-2 and WISH cells, the permeability ofunmodified and poly(ethylene glycol) (PEG)-modified (PEGylated) nanoparticles was measured inmonoculture (Caco-2) and co-culture (Caco-2/HT29-MTX) cell monolayers. Results indicated thatthe nanoparticles cross the intestinal epithelium mainly by the paracellular route. Finally, the studyof the oral pharmacokinetics of nanoencapsulated IFN in BalbC mice revealed two maxima andarea-under-the-curve of 56.9 pg*h/mL.
Fil: Imperiale, Julieta Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina
Fil: Schlachet, Inbar. Technion - Israel Institute of Technology; Israel
Fil: Lewicki, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Sosnik, Alejandro Dario. Technion - Israel Institute of Technology; Israel
Fil: Biglione, Mirna Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas en Retrovirus y Sida. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas en Retrovirus y Sida; Argentina
description Abstract: Interferon alpha (IFN) is a protein drug used to treat viral infections and cancer diseases. Dueto its poor stability in the gastrointestinal tract, only parenteral administration ensures bioavailability,which is associated with severe side eects. We hypothesized that the nanoencapsulation ofIFN within nanoparticles of the mucoadhesive polysaccharide chitosan would improve theoral bioavailability of this drug. In this work, we produced IFN-loaded chitosan nanoparticlesby the ionotropic gelation method. Their hydrodynamic diameter, polydispersity index andconcentration were characterized by dynamic light scattering and nanoparticle tracking analysis.After confirming their good cell compatibility in Caco-2 and WISH cells, the permeability ofunmodified and poly(ethylene glycol) (PEG)-modified (PEGylated) nanoparticles was measured inmonoculture (Caco-2) and co-culture (Caco-2/HT29-MTX) cell monolayers. Results indicated thatthe nanoparticles cross the intestinal epithelium mainly by the paracellular route. Finally, the studyof the oral pharmacokinetics of nanoencapsulated IFN in BalbC mice revealed two maxima andarea-under-the-curve of 56.9 pg*h/mL.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/122998
Imperiale, Julieta Celeste; Schlachet, Inbar; Lewicki, Marianela; Sosnik, Alejandro Dario; Biglione, Mirna Marcela; Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha; MDPI AG; Polymers; 11; 11; 11-2019; 1-13
2073-4360
CONICET Digital
CONICET
url http://hdl.handle.net/11336/122998
identifier_str_mv Imperiale, Julieta Celeste; Schlachet, Inbar; Lewicki, Marianela; Sosnik, Alejandro Dario; Biglione, Mirna Marcela; Oral Pharmacokinetics of a Chitosan-Based Nano- Drug Delivery System of Interferon Alpha; MDPI AG; Polymers; 11; 11; 11-2019; 1-13
2073-4360
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2073-4360/11/11/1862
info:eu-repo/semantics/altIdentifier/doi/10.3390/polym11111862
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269598664097792
score 13.13397

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