Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
- Autores
- Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant.
Fil: Puga Molina, Lis del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina - Materia
-
Thallium
Luminol
Hydrogen Peroxide
Chemiluminescence - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/4528
Ver los metadatos del registro completo
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Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidantPuga Molina, Lis del CarmenVerstraeten, Sandra VivianaThalliumLuminolHydrogen PeroxideChemiluminescencehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant.Fil: Puga Molina, Lis del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaElsevier Science2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4528Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana; Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant; Elsevier Science; Journal Of Luminescence; 137; 5-2013; 191-1970022-2313enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.039info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231312007594info:eu-repo/semantics/altIdentifier/issn/0022-2313info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:01Zoai:ri.conicet.gov.ar:11336/4528instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:01.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
title |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
spellingShingle |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant Puga Molina, Lis del Carmen Thallium Luminol Hydrogen Peroxide Chemiluminescence |
title_short |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
title_full |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
title_fullStr |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
title_full_unstemmed |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
title_sort |
Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant |
dc.creator.none.fl_str_mv |
Puga Molina, Lis del Carmen Verstraeten, Sandra Viviana |
author |
Puga Molina, Lis del Carmen |
author_facet |
Puga Molina, Lis del Carmen Verstraeten, Sandra Viviana |
author_role |
author |
author2 |
Verstraeten, Sandra Viviana |
author2_role |
author |
dc.subject.none.fl_str_mv |
Thallium Luminol Hydrogen Peroxide Chemiluminescence |
topic |
Thallium Luminol Hydrogen Peroxide Chemiluminescence |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant. Fil: Puga Molina, Lis del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina |
description |
The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/4528 Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana; Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant; Elsevier Science; Journal Of Luminescence; 137; 5-2013; 191-197 0022-2313 |
url |
http://hdl.handle.net/11336/4528 |
identifier_str_mv |
Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana; Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant; Elsevier Science; Journal Of Luminescence; 137; 5-2013; 191-197 0022-2313 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.039 info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231312007594 info:eu-repo/semantics/altIdentifier/issn/0022-2313 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614158539030528 |
score |
13.070432 |