Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant

Autores
Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant.
Fil: Puga Molina, Lis del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Materia
Thallium
Luminol
Hydrogen Peroxide
Chemiluminescence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4528

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network_name_str CONICET Digital (CONICET)
spelling Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidantPuga Molina, Lis del CarmenVerstraeten, Sandra VivianaThalliumLuminolHydrogen PeroxideChemiluminescencehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant.Fil: Puga Molina, Lis del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaFil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; ArgentinaElsevier Science2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4528Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana; Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant; Elsevier Science; Journal Of Luminescence; 137; 5-2013; 191-1970022-2313enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.039info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231312007594info:eu-repo/semantics/altIdentifier/issn/0022-2313info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:01Zoai:ri.conicet.gov.ar:11336/4528instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:01.692CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
title Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
spellingShingle Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
Puga Molina, Lis del Carmen
Thallium
Luminol
Hydrogen Peroxide
Chemiluminescence
title_short Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
title_full Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
title_fullStr Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
title_full_unstemmed Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
title_sort Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant
dc.creator.none.fl_str_mv Puga Molina, Lis del Carmen
Verstraeten, Sandra Viviana
author Puga Molina, Lis del Carmen
author_facet Puga Molina, Lis del Carmen
Verstraeten, Sandra Viviana
author_role author
author2 Verstraeten, Sandra Viviana
author2_role author
dc.subject.none.fl_str_mv Thallium
Luminol
Hydrogen Peroxide
Chemiluminescence
topic Thallium
Luminol
Hydrogen Peroxide
Chemiluminescence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant.
Fil: Puga Molina, Lis del Carmen. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
Fil: Verstraeten, Sandra Viviana. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Fisicoquímica Biológicas; Argentina
description The possibility that the reaction between Tl(III) (10–75 μM) and luminol at physiological pH requires the presence of a co-oxidant to produce chemiluminescence (CL) was evaluated. At pH 7.4 Tl(III) did not produce CL, unless the media was added with H2O2. This effect linearly depended both on Tl(III) (10–75 μM) and H2O2 (0.1–1 mM) concentrations. In the absence of agitation CL kinetics followed an oscillatory pattern, resembling a Belousov–Zhabotinski reaction. The optimal pH for the reaction was 7 with no CL detected below pH 6.5. The optimal temperature range for the reaction was 20–30 °C, decreasing at higher temperatures and being undetectable beyond 45 °C. The amount of Tl(I) recovered from samples matched the theoretical amount of Tl(OH)2+ in samples, suggesting that this species may be responsible for the reaction. Proposed mechanism indicates that Tl(III) forms a complex with luminol with an apparent association constant (K) estimated to be 1.95×1010 M−1. Superoxide anion was generated along the reaction, and the addition of superoxide dismutase decreased but not abolished CL. In summary, Tl(III) can be detected at micromolar concentrations at physiological pH through the reaction with luminol, provided H2O2 is present as a co-oxidant.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4528
Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana; Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant; Elsevier Science; Journal Of Luminescence; 137; 5-2013; 191-197
0022-2313
url http://hdl.handle.net/11336/4528
identifier_str_mv Puga Molina, Lis del Carmen; Verstraeten, Sandra Viviana; Detection of Tl(III) with luminol at physiological pH requires hydrogen peroxide as co-oxidant; Elsevier Science; Journal Of Luminescence; 137; 5-2013; 191-197
0022-2313
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jlumin.2012.12.039
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0022231312007594
info:eu-repo/semantics/altIdentifier/issn/0022-2313
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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