Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta

Autores
Desantadina, Ramiro; Quintana, Silvina; Recavarren, Mariana Ines; Relling, Alejandro Enrique
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.
Fil: Desantadina, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Quintana, Silvina. Laboratorio Bioquímico Fares Taie; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio Bioquímico Fares Taie; Argentina
Fil: Relling, Alejandro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
Fatty acid binding protein
Fetal programming
Fatty acids.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94160
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94160
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of time of gestation on fatty acid transporters mRNA expression in bovine placentaEfeito do tempo de gestação em expressão de arnm de transportadores de ácidos graxos mRNA em placenta bovinosDesantadina, RamiroQuintana, SilvinaRecavarren, Mariana InesRelling, Alejandro EnriqueFatty acid binding proteinFetal programmingFatty acids.https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.Fil: Desantadina, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Quintana, Silvina. Laboratorio Bioquímico Fares Taie; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio Bioquímico Fares Taie; ArgentinaFil: Relling, Alejandro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaUniv Federal Uberlandia2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94160Desantadina, Ramiro; Quintana, Silvina; Recavarren, Mariana Ines; Relling, Alejandro Enrique; Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta; Univ Federal Uberlandia; Bioscience Journal; 34; 1; 1-2018; 180-1851516-37251981-3163CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.seer.ufu.br/index.php/biosciencejournal/article/view/36825info:eu-repo/semantics/altIdentifier/doi/10.14393/BJ-v34n1a2018-36825info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:54Zoai:ri.conicet.gov.ar:11336/94160instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:54.983CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
Efeito do tempo de gestação em expressão de arnm de transportadores de ácidos graxos mRNA em placenta bovinos
title Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
spellingShingle Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
Desantadina, Ramiro
Fatty acid binding protein
Fetal programming
Fatty acids.
title_short Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
title_full Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
title_fullStr Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
title_full_unstemmed Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
title_sort Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta
dc.creator.none.fl_str_mv Desantadina, Ramiro
Quintana, Silvina
Recavarren, Mariana Ines
Relling, Alejandro Enrique
author Desantadina, Ramiro
author_facet Desantadina, Ramiro
Quintana, Silvina
Recavarren, Mariana Ines
Relling, Alejandro Enrique
author_role author
author2 Quintana, Silvina
Recavarren, Mariana Ines
Relling, Alejandro Enrique
author2_role author
author
author
dc.subject.none.fl_str_mv Fatty acid binding protein
Fetal programming
Fatty acids.
topic Fatty acid binding protein
Fetal programming
Fatty acids.
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.
Fil: Desantadina, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Quintana, Silvina. Laboratorio Bioquímico Fares Taie; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio Bioquímico Fares Taie; Argentina
Fil: Relling, Alejandro Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description The objective of the study was to evaluate the effect of time of gestation on fatty acid transporter mRNA expression in maternal and fetal bovine placenta. Placentas from twelve cows at different thirds of gestation (n=4 per third) were sampled at slaughter to measure FATP-1, FATP-4, FABP-1 mRNA concentration in maternal (caruncles) and fetal (cotyledons) side. Once the placenta was removed, 1cm2 was dissected and, divided into caruncles and cotyledons, stored in sterile tubes, dropped into liquid nitrogen and kept at -80° C until rtPCR analysis. Data were analyzed as a complete randomized design with a 3 x 2 factorial arrangement, using the mixed procedure (SAS 9.3) with repeated measurements on space. Time of gestation, side of the placenta and their interaction were fixed factors, whereas animal was a random factor. There was a time by treatment interaction (P < 0.01) on FATP-1 mRNA expression due to a greater mRNA expression in cotyledons on the first third of gestation as compared with the concentration in caruncles. On the second and third stages of gestation, the mRNA concentration in cotyledons decreased, reaching a similar concentration to that observed in caruncles. Fatty acid transport protein -4 and FABP-1 mRNA concentration were not different (P >0.1). We conclude that FATP-1 might play an important role in fatty acid transport during early fetal development.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94160
Desantadina, Ramiro; Quintana, Silvina; Recavarren, Mariana Ines; Relling, Alejandro Enrique; Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta; Univ Federal Uberlandia; Bioscience Journal; 34; 1; 1-2018; 180-185
1516-3725
1981-3163
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94160
identifier_str_mv Desantadina, Ramiro; Quintana, Silvina; Recavarren, Mariana Ines; Relling, Alejandro Enrique; Effect of time of gestation on fatty acid transporters mRNA expression in bovine placenta; Univ Federal Uberlandia; Bioscience Journal; 34; 1; 1-2018; 180-185
1516-3725
1981-3163
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.seer.ufu.br/index.php/biosciencejournal/article/view/36825
info:eu-repo/semantics/altIdentifier/doi/10.14393/BJ-v34n1a2018-36825
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Univ Federal Uberlandia
publisher.none.fl_str_mv Univ Federal Uberlandia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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