Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)

Autores
Varayoud, Jorgelina Guadalupe; Monje, Lucas Daniel; Moreno Piovano, Guillermo Samuel; Galoppo, Germán Hugo; Luque, Enrique Hugo; Muñoz de Toro, Monica Milagros; Ramos, Jorge Guillermo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In mammals, estrogens have been described as endocrine and paracrine modulators of neuronal differentiation and synapse formation. However, the functional role of circulating estrogens and the distribution of estrogen receptors (ERs) in the cerebral cortex of reptiles have not been clearly established. Caiman latirostris (C. latirostris) is a South American species that presents temperature-dependent sex determination (TSD). By using immunohistochemistry, we have studied the distribution of ERα in the cerebral cortex of neonatal caimans. We studied brain samples from ten-day-old TSD-females and TSD-males and from female caimans that were administered estradiol during embryonic development (hormone-dependent sex determination, HSD-females). ERα was detected in the medial (MC), dorsal (DC) and lateral (LC) cortices. ERα expression in the MC showed sex-associated differences, being significantly greater in TSD-females compared to TSD-males. Interestingly, the highest ERα expression in the MC was exhibited by HSD-females. In addition, the circulating levels of estradiol were significantly higher in females (both TSD and HSD) than in TSD-males. Double immunostaining showed that ERα is expressed by neural precursor cells (as detected by ERα/doublecortin or ERα/glial fibrillary acidic protein) and mature neurons (ERα/neuron-specific nuclear protein). Our results demonstrate that the expression of ERα in the neonatal caiman cortex is sexually dimorphic and is present in the early stages of neuronal differentiation.
Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Monje, Lucas Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Moreno Piovano, Guillermo Samuel. Universidad Nacional del Litoral; Argentina
Fil: Galoppo, Germán Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral; Argentina
Fil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Ramos, Jorge Guillermo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Materia
CEREBRAL CORTEX
CAIMAN LATIROSTRIS
IMMUNOHISTOCHEMISTRY
ESTROGEN RECEPTOR-ALPHA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96485
Acceder
id CONICETDig_9475d37980098bf6d205fc92bb274652
oai_identifier_str oai:ri.conicet.gov.ar:11336/96485
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)Varayoud, Jorgelina GuadalupeMonje, Lucas DanielMoreno Piovano, Guillermo SamuelGaloppo, Germán HugoLuque, Enrique HugoMuñoz de Toro, Monica MilagrosRamos, Jorge GuillermoCEREBRAL CORTEXCAIMAN LATIROSTRISIMMUNOHISTOCHEMISTRYESTROGEN RECEPTOR-ALPHAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In mammals, estrogens have been described as endocrine and paracrine modulators of neuronal differentiation and synapse formation. However, the functional role of circulating estrogens and the distribution of estrogen receptors (ERs) in the cerebral cortex of reptiles have not been clearly established. Caiman latirostris (C. latirostris) is a South American species that presents temperature-dependent sex determination (TSD). By using immunohistochemistry, we have studied the distribution of ERα in the cerebral cortex of neonatal caimans. We studied brain samples from ten-day-old TSD-females and TSD-males and from female caimans that were administered estradiol during embryonic development (hormone-dependent sex determination, HSD-females). ERα was detected in the medial (MC), dorsal (DC) and lateral (LC) cortices. ERα expression in the MC showed sex-associated differences, being significantly greater in TSD-females compared to TSD-males. Interestingly, the highest ERα expression in the MC was exhibited by HSD-females. In addition, the circulating levels of estradiol were significantly higher in females (both TSD and HSD) than in TSD-males. Double immunostaining showed that ERα is expressed by neural precursor cells (as detected by ERα/doublecortin or ERα/glial fibrillary acidic protein) and mature neurons (ERα/neuron-specific nuclear protein). Our results demonstrate that the expression of ERα in the neonatal caiman cortex is sexually dimorphic and is present in the early stages of neuronal differentiation.Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Monje, Lucas Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Moreno Piovano, Guillermo Samuel. Universidad Nacional del Litoral; ArgentinaFil: Galoppo, Germán Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Luque, Enrique Hugo. Universidad Nacional del Litoral; ArgentinaFil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Ramos, Jorge Guillermo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaAcademic Press Inc Elsevier Science2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96485Varayoud, Jorgelina Guadalupe; Monje, Lucas Daniel; Moreno Piovano, Guillermo Samuel; Galoppo, Germán Hugo; Luque, Enrique Hugo; et al.; Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae); Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 179; 2; 11-2012; 205-2130016-6480CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2012.08.019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T13:01:28Zoai:ri.conicet.gov.ar:11336/96485instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 13:01:28.548CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
title Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
spellingShingle Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
Varayoud, Jorgelina Guadalupe
CEREBRAL CORTEX
CAIMAN LATIROSTRIS
IMMUNOHISTOCHEMISTRY
ESTROGEN RECEPTOR-ALPHA
title_short Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
title_full Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
title_fullStr Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
title_full_unstemmed Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
title_sort Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae)
dc.creator.none.fl_str_mv Varayoud, Jorgelina Guadalupe
Monje, Lucas Daniel
Moreno Piovano, Guillermo Samuel
Galoppo, Germán Hugo
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
Ramos, Jorge Guillermo
author Varayoud, Jorgelina Guadalupe
author_facet Varayoud, Jorgelina Guadalupe
Monje, Lucas Daniel
Moreno Piovano, Guillermo Samuel
Galoppo, Germán Hugo
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
Ramos, Jorge Guillermo
author_role author
author2 Monje, Lucas Daniel
Moreno Piovano, Guillermo Samuel
Galoppo, Germán Hugo
Luque, Enrique Hugo
Muñoz de Toro, Monica Milagros
Ramos, Jorge Guillermo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CEREBRAL CORTEX
CAIMAN LATIROSTRIS
IMMUNOHISTOCHEMISTRY
ESTROGEN RECEPTOR-ALPHA
topic CEREBRAL CORTEX
CAIMAN LATIROSTRIS
IMMUNOHISTOCHEMISTRY
ESTROGEN RECEPTOR-ALPHA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In mammals, estrogens have been described as endocrine and paracrine modulators of neuronal differentiation and synapse formation. However, the functional role of circulating estrogens and the distribution of estrogen receptors (ERs) in the cerebral cortex of reptiles have not been clearly established. Caiman latirostris (C. latirostris) is a South American species that presents temperature-dependent sex determination (TSD). By using immunohistochemistry, we have studied the distribution of ERα in the cerebral cortex of neonatal caimans. We studied brain samples from ten-day-old TSD-females and TSD-males and from female caimans that were administered estradiol during embryonic development (hormone-dependent sex determination, HSD-females). ERα was detected in the medial (MC), dorsal (DC) and lateral (LC) cortices. ERα expression in the MC showed sex-associated differences, being significantly greater in TSD-females compared to TSD-males. Interestingly, the highest ERα expression in the MC was exhibited by HSD-females. In addition, the circulating levels of estradiol were significantly higher in females (both TSD and HSD) than in TSD-males. Double immunostaining showed that ERα is expressed by neural precursor cells (as detected by ERα/doublecortin or ERα/glial fibrillary acidic protein) and mature neurons (ERα/neuron-specific nuclear protein). Our results demonstrate that the expression of ERα in the neonatal caiman cortex is sexually dimorphic and is present in the early stages of neuronal differentiation.
Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Monje, Lucas Daniel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Moreno Piovano, Guillermo Samuel. Universidad Nacional del Litoral; Argentina
Fil: Galoppo, Germán Hugo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Luque, Enrique Hugo. Universidad Nacional del Litoral; Argentina
Fil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina
Fil: Ramos, Jorge Guillermo. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
description In mammals, estrogens have been described as endocrine and paracrine modulators of neuronal differentiation and synapse formation. However, the functional role of circulating estrogens and the distribution of estrogen receptors (ERs) in the cerebral cortex of reptiles have not been clearly established. Caiman latirostris (C. latirostris) is a South American species that presents temperature-dependent sex determination (TSD). By using immunohistochemistry, we have studied the distribution of ERα in the cerebral cortex of neonatal caimans. We studied brain samples from ten-day-old TSD-females and TSD-males and from female caimans that were administered estradiol during embryonic development (hormone-dependent sex determination, HSD-females). ERα was detected in the medial (MC), dorsal (DC) and lateral (LC) cortices. ERα expression in the MC showed sex-associated differences, being significantly greater in TSD-females compared to TSD-males. Interestingly, the highest ERα expression in the MC was exhibited by HSD-females. In addition, the circulating levels of estradiol were significantly higher in females (both TSD and HSD) than in TSD-males. Double immunostaining showed that ERα is expressed by neural precursor cells (as detected by ERα/doublecortin or ERα/glial fibrillary acidic protein) and mature neurons (ERα/neuron-specific nuclear protein). Our results demonstrate that the expression of ERα in the neonatal caiman cortex is sexually dimorphic and is present in the early stages of neuronal differentiation.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96485
Varayoud, Jorgelina Guadalupe; Monje, Lucas Daniel; Moreno Piovano, Guillermo Samuel; Galoppo, Germán Hugo; Luque, Enrique Hugo; et al.; Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae); Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 179; 2; 11-2012; 205-213
0016-6480
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96485
identifier_str_mv Varayoud, Jorgelina Guadalupe; Monje, Lucas Daniel; Moreno Piovano, Guillermo Samuel; Galoppo, Germán Hugo; Luque, Enrique Hugo; et al.; Sexually dimorphic expression of receptor-alpha in the cerebral cortex of neonatal Caiman latirostris (Crocodylia, Aligatoridae); Academic Press Inc Elsevier Science; General and Comparative Endocrinology; 179; 2; 11-2012; 205-213
0016-6480
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ygcen.2012.08.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.747614

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