In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms

Autores
Marani, Mariela Mirta; Costa, Joana; Mafra, Isabel; Oliveira, Maria Beatriz P. P.; Camperi, Silvia Andrea; Leite, José Roberto de Souza Almeida
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation of the referred protein was performed. The main objective of this study was the selection of a set of peptides that could function as potential immunogens for the production of novel antibodies against CP4-EPSPS protein. For this purpose, the protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively analyzed for further selection of the best candidates for antibody production. The analysis enabled the successful proposal of four peptides with potential immunogenicity for their future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and, subsequently, to generate adequate antibodies for CP4-EPSPS recognition. The present work will be followed by the synthesis of the candidate peptides to be incubated in animals for antibody generation and potential applicability for the development of an immunosensor for CP4-EPSPS detection.
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina
Fil: Costa, Joana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina. Universidade do Porto; Portugal
Fil: Mafra, Isabel. Universidade do Porto; Portugal
Fil: Oliveira, Maria Beatriz P. P.. Universidade do Porto; Portugal
Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires; Argentina
Fil: Leite, José Roberto de Souza Almeida. Universidade Federal do Piauí; Brasil
Materia
Gmo
In Silico
Epitope Prediction
Cp4-Epsps
Bioinformatics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/19185
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/19185
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified OrganismsMarani, Mariela MirtaCosta, JoanaMafra, IsabelOliveira, Maria Beatriz P. P.Camperi, Silvia AndreaLeite, José Roberto de Souza AlmeidaGmoIn SilicoEpitope PredictionCp4-EpspsBioinformaticshttps://purl.org/becyt/ford/1.2https://purl.org/becyt/ford/1For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation of the referred protein was performed. The main objective of this study was the selection of a set of peptides that could function as potential immunogens for the production of novel antibodies against CP4-EPSPS protein. For this purpose, the protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively analyzed for further selection of the best candidates for antibody production. The analysis enabled the successful proposal of four peptides with potential immunogenicity for their future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and, subsequently, to generate adequate antibodies for CP4-EPSPS recognition. The present work will be followed by the synthesis of the candidate peptides to be incubated in animals for antibody generation and potential applicability for the development of an immunosensor for CP4-EPSPS detection.Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; ArgentinaFil: Costa, Joana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina. Universidade do Porto; PortugalFil: Mafra, Isabel. Universidade do Porto; PortugalFil: Oliveira, Maria Beatriz P. P.. Universidade do Porto; PortugalFil: Camperi, Silvia Andrea. Universidad de Buenos Aires; ArgentinaFil: Leite, José Roberto de Souza Almeida. Universidade Federal do Piauí; BrasilWiley2015-01-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/19185Marani, Mariela Mirta; Costa, Joana; Mafra, Isabel; Oliveira, Maria Beatriz P. P.; Camperi, Silvia Andrea; et al.; In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms; Wiley; Journal Of Peptide Science; 104; 2; 23-1-2015; 91-1001099-1387CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/bip.22609/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/bip.22609info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:49Zoai:ri.conicet.gov.ar:11336/19185instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:50.157CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
title In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
spellingShingle In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
Marani, Mariela Mirta
Gmo
In Silico
Epitope Prediction
Cp4-Epsps
Bioinformatics
title_short In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
title_full In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
title_fullStr In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
title_full_unstemmed In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
title_sort In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms
dc.creator.none.fl_str_mv Marani, Mariela Mirta
Costa, Joana
Mafra, Isabel
Oliveira, Maria Beatriz P. P.
Camperi, Silvia Andrea
Leite, José Roberto de Souza Almeida
author Marani, Mariela Mirta
author_facet Marani, Mariela Mirta
Costa, Joana
Mafra, Isabel
Oliveira, Maria Beatriz P. P.
Camperi, Silvia Andrea
Leite, José Roberto de Souza Almeida
author_role author
author2 Costa, Joana
Mafra, Isabel
Oliveira, Maria Beatriz P. P.
Camperi, Silvia Andrea
Leite, José Roberto de Souza Almeida
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Gmo
In Silico
Epitope Prediction
Cp4-Epsps
Bioinformatics
topic Gmo
In Silico
Epitope Prediction
Cp4-Epsps
Bioinformatics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.2
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation of the referred protein was performed. The main objective of this study was the selection of a set of peptides that could function as potential immunogens for the production of novel antibodies against CP4-EPSPS protein. For this purpose, the protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively analyzed for further selection of the best candidates for antibody production. The analysis enabled the successful proposal of four peptides with potential immunogenicity for their future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and, subsequently, to generate adequate antibodies for CP4-EPSPS recognition. The present work will be followed by the synthesis of the candidate peptides to be incubated in animals for antibody generation and potential applicability for the development of an immunosensor for CP4-EPSPS detection.
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina
Fil: Costa, Joana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico; Argentina. Universidade do Porto; Portugal
Fil: Mafra, Isabel. Universidade do Porto; Portugal
Fil: Oliveira, Maria Beatriz P. P.. Universidade do Porto; Portugal
Fil: Camperi, Silvia Andrea. Universidad de Buenos Aires; Argentina
Fil: Leite, José Roberto de Souza Almeida. Universidade Federal do Piauí; Brasil
description For the prospective immunorecognition of 5-enolpyruvylshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, an extensive in silico evaluation of the referred protein was performed. The main objective of this study was the selection of a set of peptides that could function as potential immunogens for the production of novel antibodies against CP4-EPSPS protein. For this purpose, the protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively analyzed for further selection of the best candidates for antibody production. The analysis enabled the successful proposal of four peptides with potential immunogenicity for their future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and, subsequently, to generate adequate antibodies for CP4-EPSPS recognition. The present work will be followed by the synthesis of the candidate peptides to be incubated in animals for antibody generation and potential applicability for the development of an immunosensor for CP4-EPSPS detection.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/19185
Marani, Mariela Mirta; Costa, Joana; Mafra, Isabel; Oliveira, Maria Beatriz P. P.; Camperi, Silvia Andrea; et al.; In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms; Wiley; Journal Of Peptide Science; 104; 2; 23-1-2015; 91-100
1099-1387
CONICET Digital
CONICET
url http://hdl.handle.net/11336/19185
identifier_str_mv Marani, Mariela Mirta; Costa, Joana; Mafra, Isabel; Oliveira, Maria Beatriz P. P.; Camperi, Silvia Andrea; et al.; In Silico Peptide Prediction for Antibody Generation to Recognize 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) in Genetically Modified Organisms; Wiley; Journal Of Peptide Science; 104; 2; 23-1-2015; 91-100
1099-1387
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/bip.22609/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/bip.22609
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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