Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients

Autores
Ortega, Francisco G.; Regiart, Matías D.; Rodríguez Martínez, Alba; De Miguel-Pérez, Diego; Serrano, María J.; Lorente, José A.; Tortella, Gonzalo; Rubilar, Olga; Sapag, Manuel Karim; Bertotti, Mauro; Fernández Baldo, Martín Alejandro
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL-1, with a wide linear range of 2 to 1000 pg mL-1, while for CD81, the limit of detection was 3 pg mL-1, with a wide linear range of 0.01 to 10 ng mL-1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.
Fil: Ortega, Francisco G.. Balearic Islands Health Research Institute; España
Fil: Regiart, Matías D.. Universidade de Sao Paulo; Brasil
Fil: Rodríguez Martínez, Alba. Universidad de Granada; España
Fil: De Miguel-Pérez, Diego. Universidad de Granada; España
Fil: Serrano, María J.. Universidad de Granada; España
Fil: Lorente, José A.. Universidad de Granada; España
Fil: Tortella, Gonzalo. Universidad de La Frontera; Chile
Fil: Rubilar, Olga. Universidad de La Frontera; Chile
Fil: Sapag, Manuel Karim. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
Fil: Bertotti, Mauro. Universidade de Sao Paulo; Brasil
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CLAUDIN 7
CD81
BIOMARKERS
BREAST CANCER
CANCER DIAGNOSIS
EXTRACELLULAR VESICLES
PAPER IMMUNOSENSOR
ELECTROCHEMICAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/138506

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oai_identifier_str oai:ri.conicet.gov.ar:11336/138506
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer PatientsOrtega, Francisco G.Regiart, Matías D.Rodríguez Martínez, AlbaDe Miguel-Pérez, DiegoSerrano, María J.Lorente, José A.Tortella, GonzaloRubilar, OlgaSapag, Manuel KarimBertotti, MauroFernández Baldo, Martín AlejandroCLAUDIN 7CD81BIOMARKERSBREAST CANCERCANCER DIAGNOSISEXTRACELLULAR VESICLESPAPER IMMUNOSENSORELECTROCHEMICALhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL-1, with a wide linear range of 2 to 1000 pg mL-1, while for CD81, the limit of detection was 3 pg mL-1, with a wide linear range of 0.01 to 10 ng mL-1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.Fil: Ortega, Francisco G.. Balearic Islands Health Research Institute; EspañaFil: Regiart, Matías D.. Universidade de Sao Paulo; BrasilFil: Rodríguez Martínez, Alba. Universidad de Granada; EspañaFil: De Miguel-Pérez, Diego. Universidad de Granada; EspañaFil: Serrano, María J.. Universidad de Granada; EspañaFil: Lorente, José A.. Universidad de Granada; EspañaFil: Tortella, Gonzalo. Universidad de La Frontera; ChileFil: Rubilar, Olga. Universidad de La Frontera; ChileFil: Sapag, Manuel Karim. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; ArgentinaFil: Bertotti, Mauro. Universidade de Sao Paulo; BrasilFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaAmerican Chemical Society2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/138506Ortega, Francisco G.; Regiart, Matías D.; Rodríguez Martínez, Alba; De Miguel-Pérez, Diego; Serrano, María J.; et al.; Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients; American Chemical Society; Analytical Chemistry; 93; 2; 12-2020; 1143-11530003-2700CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.0c04180info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.analchem.0c04180info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:38:29Zoai:ri.conicet.gov.ar:11336/138506instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:38:29.952CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
title Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
spellingShingle Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
Ortega, Francisco G.
CLAUDIN 7
CD81
BIOMARKERS
BREAST CANCER
CANCER DIAGNOSIS
EXTRACELLULAR VESICLES
PAPER IMMUNOSENSOR
ELECTROCHEMICAL
title_short Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
title_full Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
title_fullStr Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
title_full_unstemmed Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
title_sort Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
dc.creator.none.fl_str_mv Ortega, Francisco G.
Regiart, Matías D.
Rodríguez Martínez, Alba
De Miguel-Pérez, Diego
Serrano, María J.
Lorente, José A.
Tortella, Gonzalo
Rubilar, Olga
Sapag, Manuel Karim
Bertotti, Mauro
Fernández Baldo, Martín Alejandro
author Ortega, Francisco G.
author_facet Ortega, Francisco G.
Regiart, Matías D.
Rodríguez Martínez, Alba
De Miguel-Pérez, Diego
Serrano, María J.
Lorente, José A.
Tortella, Gonzalo
Rubilar, Olga
Sapag, Manuel Karim
Bertotti, Mauro
Fernández Baldo, Martín Alejandro
author_role author
author2 Regiart, Matías D.
Rodríguez Martínez, Alba
De Miguel-Pérez, Diego
Serrano, María J.
Lorente, José A.
Tortella, Gonzalo
Rubilar, Olga
Sapag, Manuel Karim
Bertotti, Mauro
Fernández Baldo, Martín Alejandro
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CLAUDIN 7
CD81
BIOMARKERS
BREAST CANCER
CANCER DIAGNOSIS
EXTRACELLULAR VESICLES
PAPER IMMUNOSENSOR
ELECTROCHEMICAL
topic CLAUDIN 7
CD81
BIOMARKERS
BREAST CANCER
CANCER DIAGNOSIS
EXTRACELLULAR VESICLES
PAPER IMMUNOSENSOR
ELECTROCHEMICAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL-1, with a wide linear range of 2 to 1000 pg mL-1, while for CD81, the limit of detection was 3 pg mL-1, with a wide linear range of 0.01 to 10 ng mL-1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.
Fil: Ortega, Francisco G.. Balearic Islands Health Research Institute; España
Fil: Regiart, Matías D.. Universidade de Sao Paulo; Brasil
Fil: Rodríguez Martínez, Alba. Universidad de Granada; España
Fil: De Miguel-Pérez, Diego. Universidad de Granada; España
Fil: Serrano, María J.. Universidad de Granada; España
Fil: Lorente, José A.. Universidad de Granada; España
Fil: Tortella, Gonzalo. Universidad de La Frontera; Chile
Fil: Rubilar, Olga. Universidad de La Frontera; Chile
Fil: Sapag, Manuel Karim. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich". Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto de Física Aplicada "Dr. Jorge Andrés Zgrablich"; Argentina
Fil: Bertotti, Mauro. Universidade de Sao Paulo; Brasil
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL-1, with a wide linear range of 2 to 1000 pg mL-1, while for CD81, the limit of detection was 3 pg mL-1, with a wide linear range of 0.01 to 10 ng mL-1. Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/138506
Ortega, Francisco G.; Regiart, Matías D.; Rodríguez Martínez, Alba; De Miguel-Pérez, Diego; Serrano, María J.; et al.; Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients; American Chemical Society; Analytical Chemistry; 93; 2; 12-2020; 1143-1153
0003-2700
CONICET Digital
CONICET
url http://hdl.handle.net/11336/138506
identifier_str_mv Ortega, Francisco G.; Regiart, Matías D.; Rodríguez Martínez, Alba; De Miguel-Pérez, Diego; Serrano, María J.; et al.; Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients; American Chemical Society; Analytical Chemistry; 93; 2; 12-2020; 1143-1153
0003-2700
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.0c04180
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.analchem.0c04180
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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