Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels

Autores
García, Isabel Mercedes; Altamirano, Liliana; Mazzei, Luciana Jorgelina; Fornes, Miguel Walter; Cuello Carrión, Fernando Darío; Ferder, León; Manucha, Walter Ariel Fernando
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: From hypertension studies, have been well characterized low levels of vitamin D linked to the renin-angiotensin system exaltation as oxidative stress. The heat shock protein 70 (Hsp70) chaperone regulates a diverse set of signaling pathways for cellular oxidative stress responses. In addition, Hsp70 has been shown to protect against Angiotensin II-induced hypertension and exert a cytoprotective effect by down-regulation of Nox4. Aims: Here, we evaluated whether vitamin D receptor (VDR) associated with Hsp70/ AT1 expression may be involved in the mechanism by which paricalcitol exerts renal protection in spontaneously hypertensive rats (SHR). Methods: One-month-old female SRH were treated with vehicle, paricalcitol, enalapril, or combination of both for 4 months. The following were determined: blood pressure; biochemical parameters; fibrosis; apoptosis; mitochondrial morphology; VDR, AT1 receptor, and Hsp70 expression in renal cortex. Results: Blood pressure was markedly reduced by enalapril or combination but not by paricalcitol alone. However, VDR activation and/or enalapril prevented fibrosis (%), the number of TUNEL-positive apoptotic cells (), mitochondrial damage and NADPH oxidase activity, in SHR. Additionally, high AT1 receptor as low Hsp70 expression (immunohistochemical/ immunofluorescence studies) were reverted in renal cortexes from SHR paricalcitol and/or enalapril-treated animals, and these changes were most marked in the combination therapy group. Finally, all parameters recovery, were consistent with an improvement in VDR expression. Conclusions: These data suggest that Hsp70/ AT1 modulated by VDR are involved in the mechanism by which paricalcitol exerts renal protection in SHR. Also, the effect of combining paricalcitol and enalapril on cytoprotection suggest a compensatory/ additive feedback system.
Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Fil: Altamirano, Liliana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza ; Argentina
Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Ferder, León. Ponce School of Medicine and Health Sciences. Department of Physiology and Pharmacology; Puerto Rico
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Materia
Hypertension
Vitamin D Receptor
Angiotensin Ii Type 1 Receptor
Heat Shock Protein 70
Renal Cytoprotection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14541
Acceder
id CONICETDig_927ee76503d2766037d1b56b888c481d
oai_identifier_str oai:ri.conicet.gov.ar:11336/14541
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levelsGarcía, Isabel MercedesAltamirano, LilianaMazzei, Luciana JorgelinaFornes, Miguel WalterCuello Carrión, Fernando DaríoFerder, LeónManucha, Walter Ariel FernandoHypertensionVitamin D ReceptorAngiotensin Ii Type 1 ReceptorHeat Shock Protein 70Renal Cytoprotectionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: From hypertension studies, have been well characterized low levels of vitamin D linked to the renin-angiotensin system exaltation as oxidative stress. The heat shock protein 70 (Hsp70) chaperone regulates a diverse set of signaling pathways for cellular oxidative stress responses. In addition, Hsp70 has been shown to protect against Angiotensin II-induced hypertension and exert a cytoprotective effect by down-regulation of Nox4. Aims: Here, we evaluated whether vitamin D receptor (VDR) associated with Hsp70/ AT1 expression may be involved in the mechanism by which paricalcitol exerts renal protection in spontaneously hypertensive rats (SHR). Methods: One-month-old female SRH were treated with vehicle, paricalcitol, enalapril, or combination of both for 4 months. The following were determined: blood pressure; biochemical parameters; fibrosis; apoptosis; mitochondrial morphology; VDR, AT1 receptor, and Hsp70 expression in renal cortex. Results: Blood pressure was markedly reduced by enalapril or combination but not by paricalcitol alone. However, VDR activation and/or enalapril prevented fibrosis (%), the number of TUNEL-positive apoptotic cells (), mitochondrial damage and NADPH oxidase activity, in SHR. Additionally, high AT1 receptor as low Hsp70 expression (immunohistochemical/ immunofluorescence studies) were reverted in renal cortexes from SHR paricalcitol and/or enalapril-treated animals, and these changes were most marked in the combination therapy group. Finally, all parameters recovery, were consistent with an improvement in VDR expression. Conclusions: These data suggest that Hsp70/ AT1 modulated by VDR are involved in the mechanism by which paricalcitol exerts renal protection in SHR. Also, the effect of combining paricalcitol and enalapril on cytoprotection suggest a compensatory/ additive feedback system.Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; ArgentinaFil: Altamirano, Liliana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; ArgentinaFil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; ArgentinaFil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza ; ArgentinaFil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Ferder, León. Ponce School of Medicine and Health Sciences. Department of Physiology and Pharmacology; Puerto RicoFil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; ArgentinaSpringer2013-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14541Manucha, Walter Ariel Fernando; Ferder, León; Cuello Carrión, Fernando Darío; Fornes, Miguel Walter; Mazzei, Luciana Jorgelina; Altamirano, Liliana; et al.; Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels; Springer; Cell Stress & Chaperones.; 19; 4; 11-2013; 479-4911355-8145enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12192-013-0474-3info:eu-repo/semantics/altIdentifier/doi/10.1007/s12192-013-0474-3info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:42:45Zoai:ri.conicet.gov.ar:11336/14541instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:42:45.73CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
title Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
spellingShingle Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
García, Isabel Mercedes
Hypertension
Vitamin D Receptor
Angiotensin Ii Type 1 Receptor
Heat Shock Protein 70
Renal Cytoprotection
title_short Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
title_full Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
title_fullStr Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
title_full_unstemmed Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
title_sort Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels
dc.creator.none.fl_str_mv García, Isabel Mercedes
Altamirano, Liliana
Mazzei, Luciana Jorgelina
Fornes, Miguel Walter
Cuello Carrión, Fernando Darío
Ferder, León
Manucha, Walter Ariel Fernando
author García, Isabel Mercedes
author_facet García, Isabel Mercedes
Altamirano, Liliana
Mazzei, Luciana Jorgelina
Fornes, Miguel Walter
Cuello Carrión, Fernando Darío
Ferder, León
Manucha, Walter Ariel Fernando
author_role author
author2 Altamirano, Liliana
Mazzei, Luciana Jorgelina
Fornes, Miguel Walter
Cuello Carrión, Fernando Darío
Ferder, León
Manucha, Walter Ariel Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hypertension
Vitamin D Receptor
Angiotensin Ii Type 1 Receptor
Heat Shock Protein 70
Renal Cytoprotection
topic Hypertension
Vitamin D Receptor
Angiotensin Ii Type 1 Receptor
Heat Shock Protein 70
Renal Cytoprotection
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: From hypertension studies, have been well characterized low levels of vitamin D linked to the renin-angiotensin system exaltation as oxidative stress. The heat shock protein 70 (Hsp70) chaperone regulates a diverse set of signaling pathways for cellular oxidative stress responses. In addition, Hsp70 has been shown to protect against Angiotensin II-induced hypertension and exert a cytoprotective effect by down-regulation of Nox4. Aims: Here, we evaluated whether vitamin D receptor (VDR) associated with Hsp70/ AT1 expression may be involved in the mechanism by which paricalcitol exerts renal protection in spontaneously hypertensive rats (SHR). Methods: One-month-old female SRH were treated with vehicle, paricalcitol, enalapril, or combination of both for 4 months. The following were determined: blood pressure; biochemical parameters; fibrosis; apoptosis; mitochondrial morphology; VDR, AT1 receptor, and Hsp70 expression in renal cortex. Results: Blood pressure was markedly reduced by enalapril or combination but not by paricalcitol alone. However, VDR activation and/or enalapril prevented fibrosis (%), the number of TUNEL-positive apoptotic cells (), mitochondrial damage and NADPH oxidase activity, in SHR. Additionally, high AT1 receptor as low Hsp70 expression (immunohistochemical/ immunofluorescence studies) were reverted in renal cortexes from SHR paricalcitol and/or enalapril-treated animals, and these changes were most marked in the combination therapy group. Finally, all parameters recovery, were consistent with an improvement in VDR expression. Conclusions: These data suggest that Hsp70/ AT1 modulated by VDR are involved in the mechanism by which paricalcitol exerts renal protection in SHR. Also, the effect of combining paricalcitol and enalapril on cytoprotection suggest a compensatory/ additive feedback system.
Fil: García, Isabel Mercedes. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Fil: Altamirano, Liliana. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Fil: Mazzei, Luciana Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
Fil: Fornes, Miguel Walter. Consejo Nacional de Investigaciones Científicas y Tecnicas. Centro Cientifico Tecnologico Mendoza. Instituto Histologia y Embriologia de Mendoza ; Argentina
Fil: Cuello Carrión, Fernando Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
Fil: Ferder, León. Ponce School of Medicine and Health Sciences. Department of Physiology and Pharmacology; Puerto Rico
Fil: Manucha, Walter Ariel Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Departamento de Patología; Argentina
description Background: From hypertension studies, have been well characterized low levels of vitamin D linked to the renin-angiotensin system exaltation as oxidative stress. The heat shock protein 70 (Hsp70) chaperone regulates a diverse set of signaling pathways for cellular oxidative stress responses. In addition, Hsp70 has been shown to protect against Angiotensin II-induced hypertension and exert a cytoprotective effect by down-regulation of Nox4. Aims: Here, we evaluated whether vitamin D receptor (VDR) associated with Hsp70/ AT1 expression may be involved in the mechanism by which paricalcitol exerts renal protection in spontaneously hypertensive rats (SHR). Methods: One-month-old female SRH were treated with vehicle, paricalcitol, enalapril, or combination of both for 4 months. The following were determined: blood pressure; biochemical parameters; fibrosis; apoptosis; mitochondrial morphology; VDR, AT1 receptor, and Hsp70 expression in renal cortex. Results: Blood pressure was markedly reduced by enalapril or combination but not by paricalcitol alone. However, VDR activation and/or enalapril prevented fibrosis (%), the number of TUNEL-positive apoptotic cells (), mitochondrial damage and NADPH oxidase activity, in SHR. Additionally, high AT1 receptor as low Hsp70 expression (immunohistochemical/ immunofluorescence studies) were reverted in renal cortexes from SHR paricalcitol and/or enalapril-treated animals, and these changes were most marked in the combination therapy group. Finally, all parameters recovery, were consistent with an improvement in VDR expression. Conclusions: These data suggest that Hsp70/ AT1 modulated by VDR are involved in the mechanism by which paricalcitol exerts renal protection in SHR. Also, the effect of combining paricalcitol and enalapril on cytoprotection suggest a compensatory/ additive feedback system.
publishDate 2013
dc.date.none.fl_str_mv 2013-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14541
Manucha, Walter Ariel Fernando; Ferder, León; Cuello Carrión, Fernando Darío; Fornes, Miguel Walter; Mazzei, Luciana Jorgelina; Altamirano, Liliana; et al.; Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels; Springer; Cell Stress & Chaperones.; 19; 4; 11-2013; 479-491
1355-8145
url http://hdl.handle.net/11336/14541
identifier_str_mv Manucha, Walter Ariel Fernando; Ferder, León; Cuello Carrión, Fernando Darío; Fornes, Miguel Walter; Mazzei, Luciana Jorgelina; Altamirano, Liliana; et al.; Vitamin D receptor-modulated Hsp70/AT1 expression may protect the kidneys of SHRs at the structural and functional levels; Springer; Cell Stress & Chaperones.; 19; 4; 11-2013; 479-491
1355-8145
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs12192-013-0474-3
info:eu-repo/semantics/altIdentifier/doi/10.1007/s12192-013-0474-3
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614461107732480
score 13.070432

Publicaciones similares