First isolation of leptospira interrogans from conepatus chinga in Argentina

Autores
Scialfa, Exequiel Alejandro; Brihuega, Bibiana; Morris, Winston Eduardo; Recavarren, Mariana Ines; Quintana, Silvina; Grunne, Sylvia; Romero, Graciela; Bolpe, Jorge; Schettino, Daniel Mateo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To identify carriers of Leptospira spp. in Buenos Aires province, Argentina, we trapped wild animals: 10 Didelphis albiventris, five Chaetophractus villosus, two Lycalopex griseus and three Conepatus chinga. Blood samples from these animals were tested by microscopic agglutination test (MAT), and one (5%: one Conepatus chinga) was positive for Leptospira Castellonis, Canicola, Grippotyphosa, Hardjo and Icterohaemorrhagiae serovars, at titers of 1:50 and 1:200. Kidneys of all animal were cultured, and one isolate of L. interrogans from a Conepatus chinga was obtained. Hamsters inoculated with this isolate strain died six days later, with several pulmonary macroscopic lesions at autopsy; the histology study revealed congestion in liver, and congestion and hemorrhage in kidney and lungs. Isolated strain was identified by serologic and molecular methods as Leptospira interrogans serogroup Canicola, with a genetic profile similar to that of Hound Utrecht IV strain.
Fil: Scialfa, Exequiel Alejandro. División Zoonosis Rurales; Argentina
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Morris, Winston Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie; Argentina
Fil: Grunne, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Romero, Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Bolpe, Jorge. Division Zoonosis Rurales; Argentina
Fil: Schettino, Daniel Mateo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Materia
CONEPATUS CHINGA
LEPTOSPIRA CANICOLA
MOLECULAR GENOTIPIFICACION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/270786
Acceder
id CONICETDig_92774921d27081f2d8cf0f5da5da68a9
oai_identifier_str oai:ri.conicet.gov.ar:11336/270786
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First isolation of leptospira interrogans from conepatus chinga in ArgentinaScialfa, Exequiel AlejandroBrihuega, BibianaMorris, Winston EduardoRecavarren, Mariana InesQuintana, SilvinaGrunne, SylviaRomero, GracielaBolpe, JorgeSchettino, Daniel MateoCONEPATUS CHINGALEPTOSPIRA CANICOLAMOLECULAR GENOTIPIFICACIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3To identify carriers of Leptospira spp. in Buenos Aires province, Argentina, we trapped wild animals: 10 Didelphis albiventris, five Chaetophractus villosus, two Lycalopex griseus and three Conepatus chinga. Blood samples from these animals were tested by microscopic agglutination test (MAT), and one (5%: one Conepatus chinga) was positive for Leptospira Castellonis, Canicola, Grippotyphosa, Hardjo and Icterohaemorrhagiae serovars, at titers of 1:50 and 1:200. Kidneys of all animal were cultured, and one isolate of L. interrogans from a Conepatus chinga was obtained. Hamsters inoculated with this isolate strain died six days later, with several pulmonary macroscopic lesions at autopsy; the histology study revealed congestion in liver, and congestion and hemorrhage in kidney and lungs. Isolated strain was identified by serologic and molecular methods as Leptospira interrogans serogroup Canicola, with a genetic profile similar to that of Hound Utrecht IV strain.Fil: Scialfa, Exequiel Alejandro. División Zoonosis Rurales; ArgentinaFil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Morris, Winston Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie; ArgentinaFil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie; ArgentinaFil: Grunne, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Romero, Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Bolpe, Jorge. Division Zoonosis Rurales; ArgentinaFil: Schettino, Daniel Mateo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaScience Park Journals2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/270786Scialfa, Exequiel Alejandro; Brihuega, Bibiana; Morris, Winston Eduardo; Recavarren, Mariana Ines; Quintana, Silvina; et al.; First isolation of leptospira interrogans from conepatus chinga in Argentina; Science Park Journals; African Journal of Applied Microbiology Research; 1; 1; 10-2012; 1-52315-6260CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://scienceparkjournals.com/AJAMR/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:56Zoai:ri.conicet.gov.ar:11336/270786instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:56.401CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First isolation of leptospira interrogans from conepatus chinga in Argentina
title First isolation of leptospira interrogans from conepatus chinga in Argentina
spellingShingle First isolation of leptospira interrogans from conepatus chinga in Argentina
Scialfa, Exequiel Alejandro
CONEPATUS CHINGA
LEPTOSPIRA CANICOLA
MOLECULAR GENOTIPIFICACION
title_short First isolation of leptospira interrogans from conepatus chinga in Argentina
title_full First isolation of leptospira interrogans from conepatus chinga in Argentina
title_fullStr First isolation of leptospira interrogans from conepatus chinga in Argentina
title_full_unstemmed First isolation of leptospira interrogans from conepatus chinga in Argentina
title_sort First isolation of leptospira interrogans from conepatus chinga in Argentina
dc.creator.none.fl_str_mv Scialfa, Exequiel Alejandro
Brihuega, Bibiana
Morris, Winston Eduardo
Recavarren, Mariana Ines
Quintana, Silvina
Grunne, Sylvia
Romero, Graciela
Bolpe, Jorge
Schettino, Daniel Mateo
author Scialfa, Exequiel Alejandro
author_facet Scialfa, Exequiel Alejandro
Brihuega, Bibiana
Morris, Winston Eduardo
Recavarren, Mariana Ines
Quintana, Silvina
Grunne, Sylvia
Romero, Graciela
Bolpe, Jorge
Schettino, Daniel Mateo
author_role author
author2 Brihuega, Bibiana
Morris, Winston Eduardo
Recavarren, Mariana Ines
Quintana, Silvina
Grunne, Sylvia
Romero, Graciela
Bolpe, Jorge
Schettino, Daniel Mateo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CONEPATUS CHINGA
LEPTOSPIRA CANICOLA
MOLECULAR GENOTIPIFICACION
topic CONEPATUS CHINGA
LEPTOSPIRA CANICOLA
MOLECULAR GENOTIPIFICACION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv To identify carriers of Leptospira spp. in Buenos Aires province, Argentina, we trapped wild animals: 10 Didelphis albiventris, five Chaetophractus villosus, two Lycalopex griseus and three Conepatus chinga. Blood samples from these animals were tested by microscopic agglutination test (MAT), and one (5%: one Conepatus chinga) was positive for Leptospira Castellonis, Canicola, Grippotyphosa, Hardjo and Icterohaemorrhagiae serovars, at titers of 1:50 and 1:200. Kidneys of all animal were cultured, and one isolate of L. interrogans from a Conepatus chinga was obtained. Hamsters inoculated with this isolate strain died six days later, with several pulmonary macroscopic lesions at autopsy; the histology study revealed congestion in liver, and congestion and hemorrhage in kidney and lungs. Isolated strain was identified by serologic and molecular methods as Leptospira interrogans serogroup Canicola, with a genetic profile similar to that of Hound Utrecht IV strain.
Fil: Scialfa, Exequiel Alejandro. División Zoonosis Rurales; Argentina
Fil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Morris, Winston Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Recavarren, Mariana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie; Argentina
Fil: Quintana, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Análisis Fares Taie; Argentina
Fil: Grunne, Sylvia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Romero, Graciela. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Bolpe, Jorge. Division Zoonosis Rurales; Argentina
Fil: Schettino, Daniel Mateo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
description To identify carriers of Leptospira spp. in Buenos Aires province, Argentina, we trapped wild animals: 10 Didelphis albiventris, five Chaetophractus villosus, two Lycalopex griseus and three Conepatus chinga. Blood samples from these animals were tested by microscopic agglutination test (MAT), and one (5%: one Conepatus chinga) was positive for Leptospira Castellonis, Canicola, Grippotyphosa, Hardjo and Icterohaemorrhagiae serovars, at titers of 1:50 and 1:200. Kidneys of all animal were cultured, and one isolate of L. interrogans from a Conepatus chinga was obtained. Hamsters inoculated with this isolate strain died six days later, with several pulmonary macroscopic lesions at autopsy; the histology study revealed congestion in liver, and congestion and hemorrhage in kidney and lungs. Isolated strain was identified by serologic and molecular methods as Leptospira interrogans serogroup Canicola, with a genetic profile similar to that of Hound Utrecht IV strain.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/270786
Scialfa, Exequiel Alejandro; Brihuega, Bibiana; Morris, Winston Eduardo; Recavarren, Mariana Ines; Quintana, Silvina; et al.; First isolation of leptospira interrogans from conepatus chinga in Argentina; Science Park Journals; African Journal of Applied Microbiology Research; 1; 1; 10-2012; 1-5
2315-6260
CONICET Digital
CONICET
url http://hdl.handle.net/11336/270786
identifier_str_mv Scialfa, Exequiel Alejandro; Brihuega, Bibiana; Morris, Winston Eduardo; Recavarren, Mariana Ines; Quintana, Silvina; et al.; First isolation of leptospira interrogans from conepatus chinga in Argentina; Science Park Journals; African Journal of Applied Microbiology Research; 1; 1; 10-2012; 1-5
2315-6260
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://scienceparkjournals.com/AJAMR/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Park Journals
publisher.none.fl_str_mv Science Park Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613263510208512
score 13.070432

Publicaciones similares