Functional changes of Cys-loop receptors generated by electromagnetic fields

Autores
Tolosa, María Fernanda; Cravero, Walter Ruben; Bouzat, Cecilia Beatriz
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Due to the rapid advances in communication technologies, the population is increasingly exposed to electromagnetic fields (EMFs). This has raised concern about potential health effects resulting from exposure to them. We here studied the influence of EMFs on AChR and 5HT3A receptors. We recorded macroscopic and single-channel currents from cells expressing these receptors and exposed to EMFs before or during agonist application. Exposure to EMFs (15 Hz-120 kHz) produces a significant decrease of the peak current, an increase on the rise time and no changes in the decay time constants of macroscopic currents activated by ACh. The peak current decreases as a function of EMF frequency (IC50 54 kHz). The effect on 5HT3A receptor currents is 3-fold more profound than on AChR currents, thus indicating different sensitivity of Cys-loop receptors to EMFs. The single-channel amplitude of AChR channels is not affected by the EMF, revealing that the reduction of macroscopic currents is originated from a change in the activation kinetics and not in the ion permeability of the receptor. In accordance with this, open time histograms change in EMF-exposed AChRs. Our results reveal that EMFs affect ligand-gated ion channel function and open doors to understand the mechanistic of such effect.
Fil: Tolosa, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cravero, Walter Ruben. Universidad Nacional del Sur. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Huerta Grande
Argentina
Sociedad Argentina de Investigación en Neurociencia
Materia
CAMPOS ELECTROMAGNETICOS
RECEPTORES CYS-LOOP
PATCH-CLAMP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242762
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Functional changes of Cys-loop receptors generated by electromagnetic fieldsTolosa, María FernandaCravero, Walter RubenBouzat, Cecilia BeatrizCAMPOS ELECTROMAGNETICOSRECEPTORES CYS-LOOPPATCH-CLAMPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Due to the rapid advances in communication technologies, the population is increasingly exposed to electromagnetic fields (EMFs). This has raised concern about potential health effects resulting from exposure to them. We here studied the influence of EMFs on AChR and 5HT3A receptors. We recorded macroscopic and single-channel currents from cells expressing these receptors and exposed to EMFs before or during agonist application. Exposure to EMFs (15 Hz-120 kHz) produces a significant decrease of the peak current, an increase on the rise time and no changes in the decay time constants of macroscopic currents activated by ACh. The peak current decreases as a function of EMF frequency (IC50 54 kHz). The effect on 5HT3A receptor currents is 3-fold more profound than on AChR currents, thus indicating different sensitivity of Cys-loop receptors to EMFs. The single-channel amplitude of AChR channels is not affected by the EMF, revealing that the reduction of macroscopic currents is originated from a change in the activation kinetics and not in the ion permeability of the receptor. In accordance with this, open time histograms change in EMF-exposed AChRs. Our results reveal that EMFs affect ligand-gated ion channel function and open doors to understand the mechanistic of such effect.Fil: Tolosa, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Cravero, Walter Ruben. Universidad Nacional del Sur. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaXXVI Congreso Anual de la Sociedad Argentina de Investigación en NeurocienciaHuerta GrandeArgentinaSociedad Argentina de Investigación en NeurocienciaSociedad Argentina de Investigación en Neurociencia2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242762Functional changes of Cys-loop receptors generated by electromagnetic fields; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Huerta Grande; Argentina; 2011; 236-236CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:30Zoai:ri.conicet.gov.ar:11336/242762instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:30.251CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Functional changes of Cys-loop receptors generated by electromagnetic fields
title Functional changes of Cys-loop receptors generated by electromagnetic fields
spellingShingle Functional changes of Cys-loop receptors generated by electromagnetic fields
Tolosa, María Fernanda
CAMPOS ELECTROMAGNETICOS
RECEPTORES CYS-LOOP
PATCH-CLAMP
title_short Functional changes of Cys-loop receptors generated by electromagnetic fields
title_full Functional changes of Cys-loop receptors generated by electromagnetic fields
title_fullStr Functional changes of Cys-loop receptors generated by electromagnetic fields
title_full_unstemmed Functional changes of Cys-loop receptors generated by electromagnetic fields
title_sort Functional changes of Cys-loop receptors generated by electromagnetic fields
dc.creator.none.fl_str_mv Tolosa, María Fernanda
Cravero, Walter Ruben
Bouzat, Cecilia Beatriz
author Tolosa, María Fernanda
author_facet Tolosa, María Fernanda
Cravero, Walter Ruben
Bouzat, Cecilia Beatriz
author_role author
author2 Cravero, Walter Ruben
Bouzat, Cecilia Beatriz
author2_role author
author
dc.subject.none.fl_str_mv CAMPOS ELECTROMAGNETICOS
RECEPTORES CYS-LOOP
PATCH-CLAMP
topic CAMPOS ELECTROMAGNETICOS
RECEPTORES CYS-LOOP
PATCH-CLAMP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Due to the rapid advances in communication technologies, the population is increasingly exposed to electromagnetic fields (EMFs). This has raised concern about potential health effects resulting from exposure to them. We here studied the influence of EMFs on AChR and 5HT3A receptors. We recorded macroscopic and single-channel currents from cells expressing these receptors and exposed to EMFs before or during agonist application. Exposure to EMFs (15 Hz-120 kHz) produces a significant decrease of the peak current, an increase on the rise time and no changes in the decay time constants of macroscopic currents activated by ACh. The peak current decreases as a function of EMF frequency (IC50 54 kHz). The effect on 5HT3A receptor currents is 3-fold more profound than on AChR currents, thus indicating different sensitivity of Cys-loop receptors to EMFs. The single-channel amplitude of AChR channels is not affected by the EMF, revealing that the reduction of macroscopic currents is originated from a change in the activation kinetics and not in the ion permeability of the receptor. In accordance with this, open time histograms change in EMF-exposed AChRs. Our results reveal that EMFs affect ligand-gated ion channel function and open doors to understand the mechanistic of such effect.
Fil: Tolosa, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cravero, Walter Ruben. Universidad Nacional del Sur. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Huerta Grande
Argentina
Sociedad Argentina de Investigación en Neurociencia
description Due to the rapid advances in communication technologies, the population is increasingly exposed to electromagnetic fields (EMFs). This has raised concern about potential health effects resulting from exposure to them. We here studied the influence of EMFs on AChR and 5HT3A receptors. We recorded macroscopic and single-channel currents from cells expressing these receptors and exposed to EMFs before or during agonist application. Exposure to EMFs (15 Hz-120 kHz) produces a significant decrease of the peak current, an increase on the rise time and no changes in the decay time constants of macroscopic currents activated by ACh. The peak current decreases as a function of EMF frequency (IC50 54 kHz). The effect on 5HT3A receptor currents is 3-fold more profound than on AChR currents, thus indicating different sensitivity of Cys-loop receptors to EMFs. The single-channel amplitude of AChR channels is not affected by the EMF, revealing that the reduction of macroscopic currents is originated from a change in the activation kinetics and not in the ion permeability of the receptor. In accordance with this, open time histograms change in EMF-exposed AChRs. Our results reveal that EMFs affect ligand-gated ion channel function and open doors to understand the mechanistic of such effect.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242762
Functional changes of Cys-loop receptors generated by electromagnetic fields; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Huerta Grande; Argentina; 2011; 236-236
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242762
identifier_str_mv Functional changes of Cys-loop receptors generated by electromagnetic fields; XXVI Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Huerta Grande; Argentina; 2011; 236-236
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencia
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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