Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems

Autores
Navarro-Nateras, L.; Diaz Gonzalez, Jancarlo; Aguas Chantes, Diana; Coria Oriundo, Lucy Linders; Battaglini, Fernando; Ventura Gallegos, José Luis; Zentella Dehesa, Alejandro; Oza, Goldie; Arriaga, L. G.; Casanova-Moreno, Jannu R.
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H2O2 diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM−1 cm−2.
Fil: Navarro-Nateras, L.. Universidad Autónoma del Estado de México; México
Fil: Diaz Gonzalez, Jancarlo. Universidad Autónoma del Estado de México; México
Fil: Aguas Chantes, Diana. Universidad Autónoma del Estado de México; México
Fil: Coria Oriundo, Lucy Linders. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
Fil: Battaglini, Fernando. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Ventura Gallegos, José Luis. Universidad Autónoma del Estado de México; México
Fil: Zentella Dehesa, Alejandro. Universidad Autónoma del Estado de México; México
Fil: Oza, Goldie. Universidad Autónoma del Estado de México; México
Fil: Arriaga, L. G.. Universidad Nacional Autónoma de México; México
Fil: Casanova-Moreno, Jannu R.. Universidad Nacional Autónoma de México; México
Materia
3D CELL CULTURE
CROSS-LINKING
EGDGE
GLUCOSE ELECTROCHEMICAL BIOSENSOR
GLUCOSE OXIDASE
GLUTARALDEHYDE
ON-CHIP EVALUATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/228537
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/228537
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture SystemsNavarro-Nateras, L.Diaz Gonzalez, JancarloAguas Chantes, DianaCoria Oriundo, Lucy LindersBattaglini, FernandoVentura Gallegos, José LuisZentella Dehesa, AlejandroOza, GoldieArriaga, L. G.Casanova-Moreno, Jannu R.3D CELL CULTURECROSS-LINKINGEGDGEGLUCOSE ELECTROCHEMICAL BIOSENSORGLUCOSE OXIDASEGLUTARALDEHYDEON-CHIP EVALUATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H2O2 diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM−1 cm−2.Fil: Navarro-Nateras, L.. Universidad Autónoma del Estado de México; MéxicoFil: Diaz Gonzalez, Jancarlo. Universidad Autónoma del Estado de México; MéxicoFil: Aguas Chantes, Diana. Universidad Autónoma del Estado de México; MéxicoFil: Coria Oriundo, Lucy Linders. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; ArgentinaFil: Battaglini, Fernando. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Ventura Gallegos, José Luis. Universidad Autónoma del Estado de México; MéxicoFil: Zentella Dehesa, Alejandro. Universidad Autónoma del Estado de México; MéxicoFil: Oza, Goldie. Universidad Autónoma del Estado de México; MéxicoFil: Arriaga, L. G.. Universidad Nacional Autónoma de México; MéxicoFil: Casanova-Moreno, Jannu R.. Universidad Nacional Autónoma de México; MéxicoMDPI2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/228537Navarro-Nateras, L.; Diaz Gonzalez, Jancarlo; Aguas Chantes, Diana; Coria Oriundo, Lucy Linders; Battaglini, Fernando; et al.; Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems; MDPI; Biosensors; 13; 6; 5-2023; 1-172079-6374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/13/6/582info:eu-repo/semantics/altIdentifier/doi/10.3390/bios13060582info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:00Zoai:ri.conicet.gov.ar:11336/228537instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:00.928CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
title Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
spellingShingle Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
Navarro-Nateras, L.
3D CELL CULTURE
CROSS-LINKING
EGDGE
GLUCOSE ELECTROCHEMICAL BIOSENSOR
GLUCOSE OXIDASE
GLUTARALDEHYDE
ON-CHIP EVALUATION
title_short Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
title_full Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
title_fullStr Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
title_full_unstemmed Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
title_sort Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems
dc.creator.none.fl_str_mv Navarro-Nateras, L.
Diaz Gonzalez, Jancarlo
Aguas Chantes, Diana
Coria Oriundo, Lucy Linders
Battaglini, Fernando
Ventura Gallegos, José Luis
Zentella Dehesa, Alejandro
Oza, Goldie
Arriaga, L. G.
Casanova-Moreno, Jannu R.
author Navarro-Nateras, L.
author_facet Navarro-Nateras, L.
Diaz Gonzalez, Jancarlo
Aguas Chantes, Diana
Coria Oriundo, Lucy Linders
Battaglini, Fernando
Ventura Gallegos, José Luis
Zentella Dehesa, Alejandro
Oza, Goldie
Arriaga, L. G.
Casanova-Moreno, Jannu R.
author_role author
author2 Diaz Gonzalez, Jancarlo
Aguas Chantes, Diana
Coria Oriundo, Lucy Linders
Battaglini, Fernando
Ventura Gallegos, José Luis
Zentella Dehesa, Alejandro
Oza, Goldie
Arriaga, L. G.
Casanova-Moreno, Jannu R.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv 3D CELL CULTURE
CROSS-LINKING
EGDGE
GLUCOSE ELECTROCHEMICAL BIOSENSOR
GLUCOSE OXIDASE
GLUTARALDEHYDE
ON-CHIP EVALUATION
topic 3D CELL CULTURE
CROSS-LINKING
EGDGE
GLUCOSE ELECTROCHEMICAL BIOSENSOR
GLUCOSE OXIDASE
GLUTARALDEHYDE
ON-CHIP EVALUATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H2O2 diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM−1 cm−2.
Fil: Navarro-Nateras, L.. Universidad Autónoma del Estado de México; México
Fil: Diaz Gonzalez, Jancarlo. Universidad Autónoma del Estado de México; México
Fil: Aguas Chantes, Diana. Universidad Autónoma del Estado de México; México
Fil: Coria Oriundo, Lucy Linders. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina
Fil: Battaglini, Fernando. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica, Analítica y Química Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Ventura Gallegos, José Luis. Universidad Autónoma del Estado de México; México
Fil: Zentella Dehesa, Alejandro. Universidad Autónoma del Estado de México; México
Fil: Oza, Goldie. Universidad Autónoma del Estado de México; México
Fil: Arriaga, L. G.. Universidad Nacional Autónoma de México; México
Fil: Casanova-Moreno, Jannu R.. Universidad Nacional Autónoma de México; México
description The inclusion of online, in situ biosensors in microfluidic cell cultures is important to monitor and characterize a physiologically mimicking environment. This work presents the performance of second-generation electrochemical enzymatic biosensors to detect glucose in cell culture media. Glutaraldehyde and ethylene glycol diglycidyl ether (EGDGE) were tested as cross-linkers to immobilize glucose oxidase and an osmium-modified redox polymer on the surface of carbon electrodes. Tests employing screen printed electrodes showed adequate performance in a Roswell Park Memorial Institute (RPMI-1640) media spiked with fetal bovine serum (FBS). Comparable first-generation sensors were shown to be heavily affected by complex biological media. This difference is explained in terms of the respective charge transfer mechanisms. Under the tested conditions, electron hopping between Os redox centers was less vulnerable than H2O2 diffusion to biofouling by the substances present in the cell culture matrix. By employing pencil leads as electrodes, the incorporation of these electrodes in a polydimethylsiloxane (PDMS) microfluidic channel was achieved simply and at a low cost. Under flow conditions, electrodes fabricated using EGDGE presented the best performance with a limit of detection of 0.5 mM, a linear range up to 10 mM, and a sensitivity of 4.69 μA mM−1 cm−2.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/228537
Navarro-Nateras, L.; Diaz Gonzalez, Jancarlo; Aguas Chantes, Diana; Coria Oriundo, Lucy Linders; Battaglini, Fernando; et al.; Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems; MDPI; Biosensors; 13; 6; 5-2023; 1-17
2079-6374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/228537
identifier_str_mv Navarro-Nateras, L.; Diaz Gonzalez, Jancarlo; Aguas Chantes, Diana; Coria Oriundo, Lucy Linders; Battaglini, Fernando; et al.; Development of a Redox-Polymer-Based Electrochemical Glucose Biosensor Suitable for Integration in Microfluidic 3D Cell Culture Systems; MDPI; Biosensors; 13; 6; 5-2023; 1-17
2079-6374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2079-6374/13/6/582
info:eu-repo/semantics/altIdentifier/doi/10.3390/bios13060582
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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