Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis

Autores
Zacarias, Silvia Mercedes; Vaccari, María; Alfano, Orlando Mario; Irazoqui, Horacio Antonio; Imoberdorf, Gustavo Eduardo
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this paper, the photocatalytic inactivation of dry spores of Bacillus subtilis dispersed on TiO2 films and irradiated with UV-A radiation (2.44–0.29mWcm-2) was studied. Experimental results indicate a minor reduction in the number of viable spores (69% of inactivation after 48 h of irradiation) when they were irradiated with UV-A radiation on borosilicate glass plates (without TiO2), confirming the resistance of spores to UV-A radiation. However, the number of viable spores significantly decreased when they were irradiated with UV-A under similar conditions but on plates coated with TiO2 (99.88% of inactivation after 24 h of irradiation), showing that dry spores of B. subtilis are vulnerable to photocatalytic inactivation. A simplified scheme was proposed to model the photocatalytic inactivation of B. subtilis, from which a kinetic expression was derived. Since the rate of photocatalytic inactivation is strongly dependent on the flux of the UV radiation on the TiO2 films, the radiation field was modeled by means of CFD software. Experimental results of spore inactivation were fitted with the derived kinetic expression, showing the inactivation rate has a square root dependence on the radiation flux reaching the photocatalyst film.
Fil: Zacarias, Silvia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Vaccari, María. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Alfano, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Fil: Irazoqui, Horacio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Imoberdorf, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Materia
BACILLUS SUBTILIS
INACTIVATION
PHOTOCATALYSIS
SPORES
UV-A
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13562
Acceder
id CONICETDig_917b56092ed67e3b3e01ffd5d3fb21ad
oai_identifier_str oai:ri.conicet.gov.ar:11336/13562
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus SubtilisZacarias, Silvia MercedesVaccari, MaríaAlfano, Orlando MarioIrazoqui, Horacio AntonioImoberdorf, Gustavo EduardoBACILLUS SUBTILISINACTIVATIONPHOTOCATALYSISSPORESUV-Ahttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2In this paper, the photocatalytic inactivation of dry spores of Bacillus subtilis dispersed on TiO2 films and irradiated with UV-A radiation (2.44–0.29mWcm-2) was studied. Experimental results indicate a minor reduction in the number of viable spores (69% of inactivation after 48 h of irradiation) when they were irradiated with UV-A radiation on borosilicate glass plates (without TiO2), confirming the resistance of spores to UV-A radiation. However, the number of viable spores significantly decreased when they were irradiated with UV-A under similar conditions but on plates coated with TiO2 (99.88% of inactivation after 24 h of irradiation), showing that dry spores of B. subtilis are vulnerable to photocatalytic inactivation. A simplified scheme was proposed to model the photocatalytic inactivation of B. subtilis, from which a kinetic expression was derived. Since the rate of photocatalytic inactivation is strongly dependent on the flux of the UV radiation on the TiO2 films, the radiation field was modeled by means of CFD software. Experimental results of spore inactivation were fitted with the derived kinetic expression, showing the inactivation rate has a square root dependence on the radiation flux reaching the photocatalyst film.Fil: Zacarias, Silvia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Vaccari, María. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Alfano, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); ArgentinaFil: Irazoqui, Horacio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Imoberdorf, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); ArgentinaElsevier Science SA2010-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13562Zacarias, Silvia Mercedes; Vaccari, María; Alfano, Orlando Mario; Irazoqui, Horacio Antonio; Imoberdorf, Gustavo Eduardo; Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 214; 2-3; 8-2010; 171-1801010-6030enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1010603010002285info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotochem.2010.06.021info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:38Zoai:ri.conicet.gov.ar:11336/13562instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:38.711CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
title Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
spellingShingle Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
Zacarias, Silvia Mercedes
BACILLUS SUBTILIS
INACTIVATION
PHOTOCATALYSIS
SPORES
UV-A
title_short Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
title_full Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
title_fullStr Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
title_full_unstemmed Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
title_sort Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis
dc.creator.none.fl_str_mv Zacarias, Silvia Mercedes
Vaccari, María
Alfano, Orlando Mario
Irazoqui, Horacio Antonio
Imoberdorf, Gustavo Eduardo
author Zacarias, Silvia Mercedes
author_facet Zacarias, Silvia Mercedes
Vaccari, María
Alfano, Orlando Mario
Irazoqui, Horacio Antonio
Imoberdorf, Gustavo Eduardo
author_role author
author2 Vaccari, María
Alfano, Orlando Mario
Irazoqui, Horacio Antonio
Imoberdorf, Gustavo Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv BACILLUS SUBTILIS
INACTIVATION
PHOTOCATALYSIS
SPORES
UV-A
topic BACILLUS SUBTILIS
INACTIVATION
PHOTOCATALYSIS
SPORES
UV-A
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv In this paper, the photocatalytic inactivation of dry spores of Bacillus subtilis dispersed on TiO2 films and irradiated with UV-A radiation (2.44–0.29mWcm-2) was studied. Experimental results indicate a minor reduction in the number of viable spores (69% of inactivation after 48 h of irradiation) when they were irradiated with UV-A radiation on borosilicate glass plates (without TiO2), confirming the resistance of spores to UV-A radiation. However, the number of viable spores significantly decreased when they were irradiated with UV-A under similar conditions but on plates coated with TiO2 (99.88% of inactivation after 24 h of irradiation), showing that dry spores of B. subtilis are vulnerable to photocatalytic inactivation. A simplified scheme was proposed to model the photocatalytic inactivation of B. subtilis, from which a kinetic expression was derived. Since the rate of photocatalytic inactivation is strongly dependent on the flux of the UV radiation on the TiO2 films, the radiation field was modeled by means of CFD software. Experimental results of spore inactivation were fitted with the derived kinetic expression, showing the inactivation rate has a square root dependence on the radiation flux reaching the photocatalyst film.
Fil: Zacarias, Silvia Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Vaccari, María. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Alfano, Orlando Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
Fil: Irazoqui, Horacio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Imoberdorf, Gustavo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico Para la Industria Química (i); Argentina
description In this paper, the photocatalytic inactivation of dry spores of Bacillus subtilis dispersed on TiO2 films and irradiated with UV-A radiation (2.44–0.29mWcm-2) was studied. Experimental results indicate a minor reduction in the number of viable spores (69% of inactivation after 48 h of irradiation) when they were irradiated with UV-A radiation on borosilicate glass plates (without TiO2), confirming the resistance of spores to UV-A radiation. However, the number of viable spores significantly decreased when they were irradiated with UV-A under similar conditions but on plates coated with TiO2 (99.88% of inactivation after 24 h of irradiation), showing that dry spores of B. subtilis are vulnerable to photocatalytic inactivation. A simplified scheme was proposed to model the photocatalytic inactivation of B. subtilis, from which a kinetic expression was derived. Since the rate of photocatalytic inactivation is strongly dependent on the flux of the UV radiation on the TiO2 films, the radiation field was modeled by means of CFD software. Experimental results of spore inactivation were fitted with the derived kinetic expression, showing the inactivation rate has a square root dependence on the radiation flux reaching the photocatalyst film.
publishDate 2010
dc.date.none.fl_str_mv 2010-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13562
Zacarias, Silvia Mercedes; Vaccari, María; Alfano, Orlando Mario; Irazoqui, Horacio Antonio; Imoberdorf, Gustavo Eduardo; Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 214; 2-3; 8-2010; 171-180
1010-6030
url http://hdl.handle.net/11336/13562
identifier_str_mv Zacarias, Silvia Mercedes; Vaccari, María; Alfano, Orlando Mario; Irazoqui, Horacio Antonio; Imoberdorf, Gustavo Eduardo; Effect of the Radiation Flux on the Photocatalytic Inactivation of Spores of Bacillus Subtilis; Elsevier Science SA; Journal of Photochemistry and Photobiology A: Chemistry; 214; 2-3; 8-2010; 171-180
1010-6030
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1010603010002285
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jphotochem.2010.06.021
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science SA
publisher.none.fl_str_mv Elsevier Science SA
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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