In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis

Autores
Falcón, Cristian Roberto; Martinez, Fernando Fabian; Carranza, Franco Alfredo; Cervi, Laura Alejandra; Motran, Claudia Cristina
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Problem The contribution of Pregnancy-specific glycoproteins (PSG), the major variant of PSG released into the circulation during pregnancy, to the pregnancy-dependent improvement of rheumatoid arthritis (RA) has still not been elucidated. Method of study Collagen-induced arthritis (CIA) was used to test the hypothesis that PSG1a when released into circulation has a modulatory role on the Th1-pathogenic response, thus improving the CIA symptoms. In vivo expression of PSG1a was induced by injection of the vaccinia (Vac)-based expression vector harboring the complete open-reading frame of PSG1a cDNA. Results In vivo PSG1a expression during the induction of CIA ameliorated the clinical symptoms, thereby reducing the arthritis score and incidence. Significantly lower levels of IL-17, IL-6, and IFN-γ, but higher levels of TGF-β and IL-10 were secreted by collagen type II-stimulated spleen mononuclear cells from Vac-PSG1a-treated mice compared with control mice. Moreover, Vac-PSG1a treatment promoted the increase in splenic CD4+CD25+Foxp3+ Treg cells. Conclusion Pre-clinical Vac-PSG1a treatment suppressed the Th1- and Th17-type-specific responses, leading to an increase in splenic Treg cells as well as IL-10- and TGF-β-secreting cells, with the CIA symptoms being ameliorated.
Fil: Falcón, Cristian Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Martinez, Fernando Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Carranza, Franco Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Motran, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
Collagen Induced Arthritis
Immunoregulation
Pregnancy Specific Glycoproteins
Regulatory T Cells
Rheumatoid Arthritis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31958
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/31958
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced ArthritisFalcón, Cristian RobertoMartinez, Fernando FabianCarranza, Franco AlfredoCervi, Laura AlejandraMotran, Claudia CristinaCollagen Induced ArthritisImmunoregulationPregnancy Specific GlycoproteinsRegulatory T CellsRheumatoid Arthritishttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Problem The contribution of Pregnancy-specific glycoproteins (PSG), the major variant of PSG released into the circulation during pregnancy, to the pregnancy-dependent improvement of rheumatoid arthritis (RA) has still not been elucidated. Method of study Collagen-induced arthritis (CIA) was used to test the hypothesis that PSG1a when released into circulation has a modulatory role on the Th1-pathogenic response, thus improving the CIA symptoms. In vivo expression of PSG1a was induced by injection of the vaccinia (Vac)-based expression vector harboring the complete open-reading frame of PSG1a cDNA. Results In vivo PSG1a expression during the induction of CIA ameliorated the clinical symptoms, thereby reducing the arthritis score and incidence. Significantly lower levels of IL-17, IL-6, and IFN-γ, but higher levels of TGF-β and IL-10 were secreted by collagen type II-stimulated spleen mononuclear cells from Vac-PSG1a-treated mice compared with control mice. Moreover, Vac-PSG1a treatment promoted the increase in splenic CD4+CD25+Foxp3+ Treg cells. Conclusion Pre-clinical Vac-PSG1a treatment suppressed the Th1- and Th17-type-specific responses, leading to an increase in splenic Treg cells as well as IL-10- and TGF-β-secreting cells, with the CIA symptoms being ameliorated.Fil: Falcón, Cristian Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Martinez, Fernando Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Carranza, Franco Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Motran, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaWiley Blackwell Publishing, Inc2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/31958Motran, Claudia Cristina; Cervi, Laura Alejandra; Carranza, Franco Alfredo; Martinez, Fernando Fabian; Falcón, Cristian Roberto; In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 72; 6; 8-2014; 527-5331046-74081600-0897CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1111/aji.12307info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/aji.12307/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:12:42Zoai:ri.conicet.gov.ar:11336/31958instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:12:42.409CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
title In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
spellingShingle In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
Falcón, Cristian Roberto
Collagen Induced Arthritis
Immunoregulation
Pregnancy Specific Glycoproteins
Regulatory T Cells
Rheumatoid Arthritis
title_short In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
title_full In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
title_fullStr In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
title_full_unstemmed In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
title_sort In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis
dc.creator.none.fl_str_mv Falcón, Cristian Roberto
Martinez, Fernando Fabian
Carranza, Franco Alfredo
Cervi, Laura Alejandra
Motran, Claudia Cristina
author Falcón, Cristian Roberto
author_facet Falcón, Cristian Roberto
Martinez, Fernando Fabian
Carranza, Franco Alfredo
Cervi, Laura Alejandra
Motran, Claudia Cristina
author_role author
author2 Martinez, Fernando Fabian
Carranza, Franco Alfredo
Cervi, Laura Alejandra
Motran, Claudia Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Collagen Induced Arthritis
Immunoregulation
Pregnancy Specific Glycoproteins
Regulatory T Cells
Rheumatoid Arthritis
topic Collagen Induced Arthritis
Immunoregulation
Pregnancy Specific Glycoproteins
Regulatory T Cells
Rheumatoid Arthritis
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Problem The contribution of Pregnancy-specific glycoproteins (PSG), the major variant of PSG released into the circulation during pregnancy, to the pregnancy-dependent improvement of rheumatoid arthritis (RA) has still not been elucidated. Method of study Collagen-induced arthritis (CIA) was used to test the hypothesis that PSG1a when released into circulation has a modulatory role on the Th1-pathogenic response, thus improving the CIA symptoms. In vivo expression of PSG1a was induced by injection of the vaccinia (Vac)-based expression vector harboring the complete open-reading frame of PSG1a cDNA. Results In vivo PSG1a expression during the induction of CIA ameliorated the clinical symptoms, thereby reducing the arthritis score and incidence. Significantly lower levels of IL-17, IL-6, and IFN-γ, but higher levels of TGF-β and IL-10 were secreted by collagen type II-stimulated spleen mononuclear cells from Vac-PSG1a-treated mice compared with control mice. Moreover, Vac-PSG1a treatment promoted the increase in splenic CD4+CD25+Foxp3+ Treg cells. Conclusion Pre-clinical Vac-PSG1a treatment suppressed the Th1- and Th17-type-specific responses, leading to an increase in splenic Treg cells as well as IL-10- and TGF-β-secreting cells, with the CIA symptoms being ameliorated.
Fil: Falcón, Cristian Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Martinez, Fernando Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Carranza, Franco Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Cervi, Laura Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Motran, Claudia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description Problem The contribution of Pregnancy-specific glycoproteins (PSG), the major variant of PSG released into the circulation during pregnancy, to the pregnancy-dependent improvement of rheumatoid arthritis (RA) has still not been elucidated. Method of study Collagen-induced arthritis (CIA) was used to test the hypothesis that PSG1a when released into circulation has a modulatory role on the Th1-pathogenic response, thus improving the CIA symptoms. In vivo expression of PSG1a was induced by injection of the vaccinia (Vac)-based expression vector harboring the complete open-reading frame of PSG1a cDNA. Results In vivo PSG1a expression during the induction of CIA ameliorated the clinical symptoms, thereby reducing the arthritis score and incidence. Significantly lower levels of IL-17, IL-6, and IFN-γ, but higher levels of TGF-β and IL-10 were secreted by collagen type II-stimulated spleen mononuclear cells from Vac-PSG1a-treated mice compared with control mice. Moreover, Vac-PSG1a treatment promoted the increase in splenic CD4+CD25+Foxp3+ Treg cells. Conclusion Pre-clinical Vac-PSG1a treatment suppressed the Th1- and Th17-type-specific responses, leading to an increase in splenic Treg cells as well as IL-10- and TGF-β-secreting cells, with the CIA symptoms being ameliorated.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31958
Motran, Claudia Cristina; Cervi, Laura Alejandra; Carranza, Franco Alfredo; Martinez, Fernando Fabian; Falcón, Cristian Roberto; In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 72; 6; 8-2014; 527-533
1046-7408
1600-0897
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31958
identifier_str_mv Motran, Claudia Cristina; Cervi, Laura Alejandra; Carranza, Franco Alfredo; Martinez, Fernando Fabian; Falcón, Cristian Roberto; In Vivo Expression of Recombinant Pregnancy-Specific Glycoprotein 1a Inhibits the Symptoms of Collagen-Induced Arthritis; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 72; 6; 8-2014; 527-533
1046-7408
1600-0897
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.1111/aji.12307
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/aji.12307/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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