TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina

Autores
Badano, Ines; Schurr, T. G.; Stietz, S. M.; Dulik, M. C.; Mampaey, M.; Quintero, I. M.; Zinovich, J. B.; Campos, Rodolfo Hector; Liotta, Domingo Javier
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study is to describe genetic variation in the TNF promoter in the ethnically diverse population of Misiones, north-eastern Argentina. We analysed 210 women including 66 Amerindians of the Mbya-Guarani ethnic group and 144 white-admixed individuals from urban and rural areas of Misiones. Their DNA samples were surveyed for TNF polymorphisms -376 A/G, -308 A/G -244 A/G and -238 A/G by PCR amplification and direct sequencing and for the Amerindian marker -857 C/T by real-time PCR. Our main findings are as follows:(i) a distinctive pattern of Single Nucleotide Polymorphism (SNP) distribution among these groups, (ii) genetic differentiation between the Mbya-Guarani and the white-admixed populations (P < 0.05), (iii) lower gene diversity (~0.05) in Mbya-Guarani compared with the whiteadmixed group (~0.21); and (iv) linkage disequilibrium between the -376A and -238A SNPs in white-admixed populations. These data highlight the principal role of population history in establishing present-day genetic variation at the TNF locus and provide a framework for undertaking ethnographic and disease association studies in Misiones.
Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: Schurr, T. G.. University of Pennsylvania; Estados Unidos de América;
Fil: Stietz, S. M.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina;
Fil: Dulik, M. C.. University of Pennsylvania; Estados Unidos de América;
Fil: Mampaey, M.. Provincia de Misiones. Ministerio de Salud Pública; Argentina;
Fil: Quintero, I. M.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina;
Fil: Zinovich, J. B.. Provincia de Misiones. Ministerio de Salud Pública; Argentina;
Fil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Materia
Tnf Promoter
Snp Variation
Amerindians And White-Admixed Women
Misiones Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1994
Acceder
id CONICETDig_911d2903ada5d7e020ffb34f5c514803
oai_identifier_str oai:ri.conicet.gov.ar:11336/1994
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, ArgentinaBadano, InesSchurr, T. G.Stietz, S. M.Dulik, M. C.Mampaey, M.Quintero, I. M.Zinovich, J. B.Campos, Rodolfo HectorLiotta, Domingo JavierTnf PromoterSnp VariationAmerindians And White-Admixed WomenMisiones Argentinahttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The aim of this study is to describe genetic variation in the TNF promoter in the ethnically diverse population of Misiones, north-eastern Argentina. We analysed 210 women including 66 Amerindians of the Mbya-Guarani ethnic group and 144 white-admixed individuals from urban and rural areas of Misiones. Their DNA samples were surveyed for TNF polymorphisms -376 A/G, -308 A/G -244 A/G and -238 A/G by PCR amplification and direct sequencing and for the Amerindian marker -857 C/T by real-time PCR. Our main findings are as follows:(i) a distinctive pattern of Single Nucleotide Polymorphism (SNP) distribution among these groups, (ii) genetic differentiation between the Mbya-Guarani and the white-admixed populations (P < 0.05), (iii) lower gene diversity (~0.05) in Mbya-Guarani compared with the whiteadmixed group (~0.21); and (iv) linkage disequilibrium between the -376A and -238A SNPs in white-admixed populations. These data highlight the principal role of population history in establishing present-day genetic variation at the TNF locus and provide a framework for undertaking ethnographic and disease association studies in Misiones.Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Fil: Schurr, T. G.. University of Pennsylvania; Estados Unidos de América;Fil: Stietz, S. M.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina;Fil: Dulik, M. C.. University of Pennsylvania; Estados Unidos de América;Fil: Mampaey, M.. Provincia de Misiones. Ministerio de Salud Pública; Argentina;Fil: Quintero, I. M.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina;Fil: Zinovich, J. B.. Provincia de Misiones. Ministerio de Salud Pública; Argentina;Fil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;Wiley2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1994Badano, Ines; Schurr, T. G.; Stietz, S. M.; Dulik, M. C.; Mampaey, M.; et al.; TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina; Wiley; International Journal of Immunogenetics; 40; 3; 6-2013; 216-2211744-313Xenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/iji.12002/abstractinfo:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/iji.12002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:09Zoai:ri.conicet.gov.ar:11336/1994instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:10.217CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
title TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
spellingShingle TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
Badano, Ines
Tnf Promoter
Snp Variation
Amerindians And White-Admixed Women
Misiones Argentina
title_short TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
title_full TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
title_fullStr TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
title_full_unstemmed TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
title_sort TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina
dc.creator.none.fl_str_mv Badano, Ines
Schurr, T. G.
Stietz, S. M.
Dulik, M. C.
Mampaey, M.
Quintero, I. M.
Zinovich, J. B.
Campos, Rodolfo Hector
Liotta, Domingo Javier
author Badano, Ines
author_facet Badano, Ines
Schurr, T. G.
Stietz, S. M.
Dulik, M. C.
Mampaey, M.
Quintero, I. M.
Zinovich, J. B.
Campos, Rodolfo Hector
Liotta, Domingo Javier
author_role author
author2 Schurr, T. G.
Stietz, S. M.
Dulik, M. C.
Mampaey, M.
Quintero, I. M.
Zinovich, J. B.
Campos, Rodolfo Hector
Liotta, Domingo Javier
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tnf Promoter
Snp Variation
Amerindians And White-Admixed Women
Misiones Argentina
topic Tnf Promoter
Snp Variation
Amerindians And White-Admixed Women
Misiones Argentina
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of this study is to describe genetic variation in the TNF promoter in the ethnically diverse population of Misiones, north-eastern Argentina. We analysed 210 women including 66 Amerindians of the Mbya-Guarani ethnic group and 144 white-admixed individuals from urban and rural areas of Misiones. Their DNA samples were surveyed for TNF polymorphisms -376 A/G, -308 A/G -244 A/G and -238 A/G by PCR amplification and direct sequencing and for the Amerindian marker -857 C/T by real-time PCR. Our main findings are as follows:(i) a distinctive pattern of Single Nucleotide Polymorphism (SNP) distribution among these groups, (ii) genetic differentiation between the Mbya-Guarani and the white-admixed populations (P < 0.05), (iii) lower gene diversity (~0.05) in Mbya-Guarani compared with the whiteadmixed group (~0.21); and (iv) linkage disequilibrium between the -376A and -238A SNPs in white-admixed populations. These data highlight the principal role of population history in establishing present-day genetic variation at the TNF locus and provide a framework for undertaking ethnographic and disease association studies in Misiones.
Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: Schurr, T. G.. University of Pennsylvania; Estados Unidos de América;
Fil: Stietz, S. M.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina;
Fil: Dulik, M. C.. University of Pennsylvania; Estados Unidos de América;
Fil: Mampaey, M.. Provincia de Misiones. Ministerio de Salud Pública; Argentina;
Fil: Quintero, I. M.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina;
Fil: Zinovich, J. B.. Provincia de Misiones. Ministerio de Salud Pública; Argentina;
Fil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
Fil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
description The aim of this study is to describe genetic variation in the TNF promoter in the ethnically diverse population of Misiones, north-eastern Argentina. We analysed 210 women including 66 Amerindians of the Mbya-Guarani ethnic group and 144 white-admixed individuals from urban and rural areas of Misiones. Their DNA samples were surveyed for TNF polymorphisms -376 A/G, -308 A/G -244 A/G and -238 A/G by PCR amplification and direct sequencing and for the Amerindian marker -857 C/T by real-time PCR. Our main findings are as follows:(i) a distinctive pattern of Single Nucleotide Polymorphism (SNP) distribution among these groups, (ii) genetic differentiation between the Mbya-Guarani and the white-admixed populations (P < 0.05), (iii) lower gene diversity (~0.05) in Mbya-Guarani compared with the whiteadmixed group (~0.21); and (iv) linkage disequilibrium between the -376A and -238A SNPs in white-admixed populations. These data highlight the principal role of population history in establishing present-day genetic variation at the TNF locus and provide a framework for undertaking ethnographic and disease association studies in Misiones.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1994
Badano, Ines; Schurr, T. G.; Stietz, S. M.; Dulik, M. C.; Mampaey, M.; et al.; TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina; Wiley; International Journal of Immunogenetics; 40; 3; 6-2013; 216-221
1744-313X
url http://hdl.handle.net/11336/1994
identifier_str_mv Badano, Ines; Schurr, T. G.; Stietz, S. M.; Dulik, M. C.; Mampaey, M.; et al.; TNF promoter SNP variation in Amerindians and white-admixed women from Misiones, Argentina; Wiley; International Journal of Immunogenetics; 40; 3; 6-2013; 216-221
1744-313X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/iji.12002/abstract
info:eu-repo/semantics/altIdentifier/doi/DOI:10.1111/iji.12002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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