FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells

Autores
Correa Garcia, Susana Raquel; Bermudez Moretti, Mariana; Travo, Adrian; Deleris, Gerard; Forfar, Isabelle
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability.
Fil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Travo, Adrian. Universite de Bordeaux; Francia
Fil: Deleris, Gerard. Universite de Bordeaux; Francia
Fil: Forfar, Isabelle. Universite de Bordeaux; Francia
Materia
Yeast
Ftir
Freeze Drying
Pls-Da
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31287
Acceder
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network_name_str CONICET Digital (CONICET)
spelling FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cellsCorrea Garcia, Susana RaquelBermudez Moretti, MarianaTravo, AdrianDeleris, GerardForfar, IsabelleYeastFtirFreeze DryingPls-Dahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability.Fil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Travo, Adrian. Universite de Bordeaux; FranciaFil: Deleris, Gerard. Universite de Bordeaux; FranciaFil: Forfar, Isabelle. Universite de Bordeaux; FranciaRoyal Society of Chemistry2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31287Correa Garcia, Susana Raquel; Bermudez Moretti, Mariana; Travo, Adrian; Deleris, Gerard; Forfar, Isabelle; FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells; Royal Society of Chemistry; Analytical Methods; 6; 6; 2-2014; 1855-18611759-9660CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1039/c3ay42322kinfo:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/c3ay42322k#!divAbstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:27Zoai:ri.conicet.gov.ar:11336/31287instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:27.662CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
spellingShingle FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
Correa Garcia, Susana Raquel
Yeast
Ftir
Freeze Drying
Pls-Da
title_short FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_full FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_fullStr FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_full_unstemmed FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
title_sort FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells
dc.creator.none.fl_str_mv Correa Garcia, Susana Raquel
Bermudez Moretti, Mariana
Travo, Adrian
Deleris, Gerard
Forfar, Isabelle
author Correa Garcia, Susana Raquel
author_facet Correa Garcia, Susana Raquel
Bermudez Moretti, Mariana
Travo, Adrian
Deleris, Gerard
Forfar, Isabelle
author_role author
author2 Bermudez Moretti, Mariana
Travo, Adrian
Deleris, Gerard
Forfar, Isabelle
author2_role author
author
author
author
dc.subject.none.fl_str_mv Yeast
Ftir
Freeze Drying
Pls-Da
topic Yeast
Ftir
Freeze Drying
Pls-Da
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability.
Fil: Correa Garcia, Susana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bermudez Moretti, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Travo, Adrian. Universite de Bordeaux; Francia
Fil: Deleris, Gerard. Universite de Bordeaux; Francia
Fil: Forfar, Isabelle. Universite de Bordeaux; Francia
description The yeast Saccharomyces cerevisiae is widely used as a biological eukaryotic model and also serves as a production organism in biotechnology. One of the methods used to avoid degradation of the yeast cell content is lyophilization. The use of lyophilized yeast cells has several advantages over fresh ones: samples can be easily transported and/or stored and variations of their metabolomic profiles do not occur during transport or storage. Fourier transform infrared (FTIR) spectroscopy is one of the most emerging approaches in modern biology that permits operation on very small quantities of whole cells without the need for extractions or purifications. This technique is very sensitive and not only allows the discrimination between different cell genotypes but also between different growth conditions. FTIR spectra provide interesting data on the metabolic status of the whole cell. Modern multivariate data processing was applied to analyse live fresh or lyophilized S. cerevisiae cells from different growth media. This study clearly demonstrates that yeast cells coming from an identical biological medium can be used indiscriminately for FTIR analysis whether they are analysed directly as live fresh cells or after lyophilization which is a freeze-drying process. Moreover, FTIR data obtained using lyophilized cells showed less variability.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31287
Correa Garcia, Susana Raquel; Bermudez Moretti, Mariana; Travo, Adrian; Deleris, Gerard; Forfar, Isabelle; FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells; Royal Society of Chemistry; Analytical Methods; 6; 6; 2-2014; 1855-1861
1759-9660
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31287
identifier_str_mv Correa Garcia, Susana Raquel; Bermudez Moretti, Mariana; Travo, Adrian; Deleris, Gerard; Forfar, Isabelle; FTIR spectroscopic metabolome analysis of lyophilized and fresh Saccharomyces cerevisiae yeast cells; Royal Society of Chemistry; Analytical Methods; 6; 6; 2-2014; 1855-1861
1759-9660
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1039/c3ay42322k
info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/c3ay42322k#!divAbstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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