The challenge of photoreceptor renewal

Autores
Politi, Luis Enrique; Volonté, Yanel Andrea; Simon, Maria Victoria; German, Olga Lorena; Rotstein, Nora Patricia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Light is critical to support life and most living animals have developed photoreceptors to detect it. This relevance is underscored by the presence of stem cells in most multi-cellular animals to replace these neurons in case of injury or disease. However, this capacity is very limited in higher vertebrates, so that neurodegenerative diseases of the retina, like retinitis pigmentosa in humans, or in the retinal degeneration (“rd”) mice, end up in blindness. We have recently shown that rat Muller glial cells (MGC) express the stem cell markers nestin and Pax6, and promote trans-differentiation of photoreceptor progenitors into multipotent stem cells, which in turn, acquire morphological and functional properties of photoreceptors. However, it is not known why MGC are unable to replace photoreceptor loss in the “rd” mice. We investigated this problem by comparing mixed neuron-glia cultures from wild type (wt) and “rd” mice. Nuclear morphology was substantially modified in “rd” MGC: in wt cultures nearly all of their nuclei showed a regular shape and less than 10% of them evidenced indentations. By contrast, in the “rd” cultures the percentage of MGC nuclei having deep indentations doubled. Moreover, nestin expression was reduced from about 80% in cultured wt MGC, to almost 40% in the “rd” cultures. Noteworthy, while in wt cultures each MGC supported about 2 photoreceptor progenitors, this number was 3 times higher in “rd” cultures, thus suggesting that this “overload” in “rd” mice might affect the availability of trophic support for photoreceptors, thus favoring their degeneration. In summary, our results suggest that, in addition to the already known defects in "rd" photoreceptors, the alterations in the structure of MGC and in their crosstalk with photoreceptors might contribute to the loss of photoreceptors and impair their possible renewal.
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Simon, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
16th International Congress on Photobiology
Córdoba
Argentina
International Congress on Photobiology.
Universidad Nacional de Córodba
Materia
PHOTORECEPTORS
REGENERATION
STEM CELLS
MULLER CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246559
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/246559
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling The challenge of photoreceptor renewalPoliti, Luis EnriqueVolonté, Yanel AndreaSimon, Maria VictoriaGerman, Olga LorenaRotstein, Nora PatriciaPHOTORECEPTORSREGENERATIONSTEM CELLSMULLER CELLShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Light is critical to support life and most living animals have developed photoreceptors to detect it. This relevance is underscored by the presence of stem cells in most multi-cellular animals to replace these neurons in case of injury or disease. However, this capacity is very limited in higher vertebrates, so that neurodegenerative diseases of the retina, like retinitis pigmentosa in humans, or in the retinal degeneration (“rd”) mice, end up in blindness. We have recently shown that rat Muller glial cells (MGC) express the stem cell markers nestin and Pax6, and promote trans-differentiation of photoreceptor progenitors into multipotent stem cells, which in turn, acquire morphological and functional properties of photoreceptors. However, it is not known why MGC are unable to replace photoreceptor loss in the “rd” mice. We investigated this problem by comparing mixed neuron-glia cultures from wild type (wt) and “rd” mice. Nuclear morphology was substantially modified in “rd” MGC: in wt cultures nearly all of their nuclei showed a regular shape and less than 10% of them evidenced indentations. By contrast, in the “rd” cultures the percentage of MGC nuclei having deep indentations doubled. Moreover, nestin expression was reduced from about 80% in cultured wt MGC, to almost 40% in the “rd” cultures. Noteworthy, while in wt cultures each MGC supported about 2 photoreceptor progenitors, this number was 3 times higher in “rd” cultures, thus suggesting that this “overload” in “rd” mice might affect the availability of trophic support for photoreceptors, thus favoring their degeneration. In summary, our results suggest that, in addition to the already known defects in "rd" photoreceptors, the alterations in the structure of MGC and in their crosstalk with photoreceptors might contribute to the loss of photoreceptors and impair their possible renewal.Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Simon, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina16th International Congress on PhotobiologyCórdobaArgentinaInternational Congress on Photobiology.Universidad Nacional de CórodbaInternational Union of Photobiology2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/246559The challenge of photoreceptor renewal; 16th International Congress on Photobiology; Córdoba; Argentina; 2014; 249-249CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://grupoargentinodefotobiologia.info/site/site/grupar/pluginfile.php/86/block_html/content/16icp-libro.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:13:09Zoai:ri.conicet.gov.ar:11336/246559instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:13:09.885CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The challenge of photoreceptor renewal
title The challenge of photoreceptor renewal
spellingShingle The challenge of photoreceptor renewal
Politi, Luis Enrique
PHOTORECEPTORS
REGENERATION
STEM CELLS
MULLER CELLS
title_short The challenge of photoreceptor renewal
title_full The challenge of photoreceptor renewal
title_fullStr The challenge of photoreceptor renewal
title_full_unstemmed The challenge of photoreceptor renewal
title_sort The challenge of photoreceptor renewal
dc.creator.none.fl_str_mv Politi, Luis Enrique
Volonté, Yanel Andrea
Simon, Maria Victoria
German, Olga Lorena
Rotstein, Nora Patricia
author Politi, Luis Enrique
author_facet Politi, Luis Enrique
Volonté, Yanel Andrea
Simon, Maria Victoria
German, Olga Lorena
Rotstein, Nora Patricia
author_role author
author2 Volonté, Yanel Andrea
Simon, Maria Victoria
German, Olga Lorena
Rotstein, Nora Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv PHOTORECEPTORS
REGENERATION
STEM CELLS
MULLER CELLS
topic PHOTORECEPTORS
REGENERATION
STEM CELLS
MULLER CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Light is critical to support life and most living animals have developed photoreceptors to detect it. This relevance is underscored by the presence of stem cells in most multi-cellular animals to replace these neurons in case of injury or disease. However, this capacity is very limited in higher vertebrates, so that neurodegenerative diseases of the retina, like retinitis pigmentosa in humans, or in the retinal degeneration (“rd”) mice, end up in blindness. We have recently shown that rat Muller glial cells (MGC) express the stem cell markers nestin and Pax6, and promote trans-differentiation of photoreceptor progenitors into multipotent stem cells, which in turn, acquire morphological and functional properties of photoreceptors. However, it is not known why MGC are unable to replace photoreceptor loss in the “rd” mice. We investigated this problem by comparing mixed neuron-glia cultures from wild type (wt) and “rd” mice. Nuclear morphology was substantially modified in “rd” MGC: in wt cultures nearly all of their nuclei showed a regular shape and less than 10% of them evidenced indentations. By contrast, in the “rd” cultures the percentage of MGC nuclei having deep indentations doubled. Moreover, nestin expression was reduced from about 80% in cultured wt MGC, to almost 40% in the “rd” cultures. Noteworthy, while in wt cultures each MGC supported about 2 photoreceptor progenitors, this number was 3 times higher in “rd” cultures, thus suggesting that this “overload” in “rd” mice might affect the availability of trophic support for photoreceptors, thus favoring their degeneration. In summary, our results suggest that, in addition to the already known defects in "rd" photoreceptors, the alterations in the structure of MGC and in their crosstalk with photoreceptors might contribute to the loss of photoreceptors and impair their possible renewal.
Fil: Politi, Luis Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Volonté, Yanel Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Simon, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: German, Olga Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Rotstein, Nora Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
16th International Congress on Photobiology
Córdoba
Argentina
International Congress on Photobiology.
Universidad Nacional de Córodba
description Light is critical to support life and most living animals have developed photoreceptors to detect it. This relevance is underscored by the presence of stem cells in most multi-cellular animals to replace these neurons in case of injury or disease. However, this capacity is very limited in higher vertebrates, so that neurodegenerative diseases of the retina, like retinitis pigmentosa in humans, or in the retinal degeneration (“rd”) mice, end up in blindness. We have recently shown that rat Muller glial cells (MGC) express the stem cell markers nestin and Pax6, and promote trans-differentiation of photoreceptor progenitors into multipotent stem cells, which in turn, acquire morphological and functional properties of photoreceptors. However, it is not known why MGC are unable to replace photoreceptor loss in the “rd” mice. We investigated this problem by comparing mixed neuron-glia cultures from wild type (wt) and “rd” mice. Nuclear morphology was substantially modified in “rd” MGC: in wt cultures nearly all of their nuclei showed a regular shape and less than 10% of them evidenced indentations. By contrast, in the “rd” cultures the percentage of MGC nuclei having deep indentations doubled. Moreover, nestin expression was reduced from about 80% in cultured wt MGC, to almost 40% in the “rd” cultures. Noteworthy, while in wt cultures each MGC supported about 2 photoreceptor progenitors, this number was 3 times higher in “rd” cultures, thus suggesting that this “overload” in “rd” mice might affect the availability of trophic support for photoreceptors, thus favoring their degeneration. In summary, our results suggest that, in addition to the already known defects in "rd" photoreceptors, the alterations in the structure of MGC and in their crosstalk with photoreceptors might contribute to the loss of photoreceptors and impair their possible renewal.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
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Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246559
The challenge of photoreceptor renewal; 16th International Congress on Photobiology; Córdoba; Argentina; 2014; 249-249
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246559
identifier_str_mv The challenge of photoreceptor renewal; 16th International Congress on Photobiology; Córdoba; Argentina; 2014; 249-249
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://grupoargentinodefotobiologia.info/site/site/grupar/pluginfile.php/86/block_html/content/16icp-libro.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv International Union of Photobiology
publisher.none.fl_str_mv International Union of Photobiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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