Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)

Autores
Podazza, Griselda; Rosa, Mariana Daniela; González, Juan Antonio; Hilal, Mirna Beatriz; Prado, Fernando Eduardo
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cadmium (Cd) uptake effects on sucrose content, invertase activities, and plasma membrane functionality were investigated in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium accumulation was significant in roots but not in shoots and leaves. Cadmium produced significant reduction in roots DW and increment in WC. Leaves and shoots did not show significant differences on both parameters. Sucrose content was higher in control roots than in Cd-exposed ones. Apoplastic sucrose content was much higher in Cd-exposed roots than in control ones. Cd-exposed roots showed a significant decrease in both cell wall-bound and cytoplasmic (neutral) invertase activities; while the vacuolar isoform did not show any change. Alterations in lipid composition and membrane fluidity of Cd-exposed roots were also observed. In Cd-exposed roots phospholipid and glycolipid contents decreased about 50%, while sterols content was reduced about 22%. Proton extrusion was inhibited by Cd. Lipid peroxidation and proton extrusion inhibition were also detected by histochemical analysis. This work findings demonstrate that Cd affects sucrose partitioning and invertase activities in apoplastic and symplastic regions in Rangpur lime roots as well as the plasma membrane functionality and H+-ATPase activity.
Fil: Podazza, Griselda. Fundación Miguel Lillo. Dirección de Botánica. Instituto de Ecología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Rosa, Mariana Daniela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: González, Juan Antonio. Fundación Miguel Lillo; Argentina
Fil: Hilal, Mirna Beatriz. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina
Fil: Prado, Fernando Eduardo. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Materia
Cd
INVERTASE
MEMBRANE
SUCROSE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/87118
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/87118
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)Podazza, GriseldaRosa, Mariana DanielaGonzález, Juan AntonioHilal, Mirna BeatrizPrado, Fernando EduardoCdINVERTASEMEMBRANESUCROSEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cadmium (Cd) uptake effects on sucrose content, invertase activities, and plasma membrane functionality were investigated in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium accumulation was significant in roots but not in shoots and leaves. Cadmium produced significant reduction in roots DW and increment in WC. Leaves and shoots did not show significant differences on both parameters. Sucrose content was higher in control roots than in Cd-exposed ones. Apoplastic sucrose content was much higher in Cd-exposed roots than in control ones. Cd-exposed roots showed a significant decrease in both cell wall-bound and cytoplasmic (neutral) invertase activities; while the vacuolar isoform did not show any change. Alterations in lipid composition and membrane fluidity of Cd-exposed roots were also observed. In Cd-exposed roots phospholipid and glycolipid contents decreased about 50%, while sterols content was reduced about 22%. Proton extrusion was inhibited by Cd. Lipid peroxidation and proton extrusion inhibition were also detected by histochemical analysis. This work findings demonstrate that Cd affects sucrose partitioning and invertase activities in apoplastic and symplastic regions in Rangpur lime roots as well as the plasma membrane functionality and H+-ATPase activity.Fil: Podazza, Griselda. Fundación Miguel Lillo. Dirección de Botánica. Instituto de Ecología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Rosa, Mariana Daniela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: González, Juan Antonio. Fundación Miguel Lillo; ArgentinaFil: Hilal, Mirna Beatriz. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; ArgentinaFil: Prado, Fernando Eduardo. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaWiley Blackwell Publishing, Inc2006-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/87118Podazza, Griselda; Rosa, Mariana Daniela; González, Juan Antonio; Hilal, Mirna Beatriz; Prado, Fernando Eduardo; Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck) ; Wiley Blackwell Publishing, Inc; Plant Biology; 8; 5; 10-2006; 706-7141435-8603CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1055/s-2006-924171info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1055/s-2006-924171info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:26Zoai:ri.conicet.gov.ar:11336/87118instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:26.591CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
title Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
spellingShingle Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
Podazza, Griselda
Cd
INVERTASE
MEMBRANE
SUCROSE
title_short Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
title_full Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
title_fullStr Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
title_full_unstemmed Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
title_sort Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck)
dc.creator.none.fl_str_mv Podazza, Griselda
Rosa, Mariana Daniela
González, Juan Antonio
Hilal, Mirna Beatriz
Prado, Fernando Eduardo
author Podazza, Griselda
author_facet Podazza, Griselda
Rosa, Mariana Daniela
González, Juan Antonio
Hilal, Mirna Beatriz
Prado, Fernando Eduardo
author_role author
author2 Rosa, Mariana Daniela
González, Juan Antonio
Hilal, Mirna Beatriz
Prado, Fernando Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cd
INVERTASE
MEMBRANE
SUCROSE
topic Cd
INVERTASE
MEMBRANE
SUCROSE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cadmium (Cd) uptake effects on sucrose content, invertase activities, and plasma membrane functionality were investigated in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium accumulation was significant in roots but not in shoots and leaves. Cadmium produced significant reduction in roots DW and increment in WC. Leaves and shoots did not show significant differences on both parameters. Sucrose content was higher in control roots than in Cd-exposed ones. Apoplastic sucrose content was much higher in Cd-exposed roots than in control ones. Cd-exposed roots showed a significant decrease in both cell wall-bound and cytoplasmic (neutral) invertase activities; while the vacuolar isoform did not show any change. Alterations in lipid composition and membrane fluidity of Cd-exposed roots were also observed. In Cd-exposed roots phospholipid and glycolipid contents decreased about 50%, while sterols content was reduced about 22%. Proton extrusion was inhibited by Cd. Lipid peroxidation and proton extrusion inhibition were also detected by histochemical analysis. This work findings demonstrate that Cd affects sucrose partitioning and invertase activities in apoplastic and symplastic regions in Rangpur lime roots as well as the plasma membrane functionality and H+-ATPase activity.
Fil: Podazza, Griselda. Fundación Miguel Lillo. Dirección de Botánica. Instituto de Ecología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Rosa, Mariana Daniela. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: González, Juan Antonio. Fundación Miguel Lillo; Argentina
Fil: Hilal, Mirna Beatriz. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina
Fil: Prado, Fernando Eduardo. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
description Cadmium (Cd) uptake effects on sucrose content, invertase activities, and plasma membrane functionality were investigated in Rangpur lime roots (Citrus limonia L. Osbeck). Cadmium accumulation was significant in roots but not in shoots and leaves. Cadmium produced significant reduction in roots DW and increment in WC. Leaves and shoots did not show significant differences on both parameters. Sucrose content was higher in control roots than in Cd-exposed ones. Apoplastic sucrose content was much higher in Cd-exposed roots than in control ones. Cd-exposed roots showed a significant decrease in both cell wall-bound and cytoplasmic (neutral) invertase activities; while the vacuolar isoform did not show any change. Alterations in lipid composition and membrane fluidity of Cd-exposed roots were also observed. In Cd-exposed roots phospholipid and glycolipid contents decreased about 50%, while sterols content was reduced about 22%. Proton extrusion was inhibited by Cd. Lipid peroxidation and proton extrusion inhibition were also detected by histochemical analysis. This work findings demonstrate that Cd affects sucrose partitioning and invertase activities in apoplastic and symplastic regions in Rangpur lime roots as well as the plasma membrane functionality and H+-ATPase activity.
publishDate 2006
dc.date.none.fl_str_mv 2006-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/87118
Podazza, Griselda; Rosa, Mariana Daniela; González, Juan Antonio; Hilal, Mirna Beatriz; Prado, Fernando Eduardo; Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck) ; Wiley Blackwell Publishing, Inc; Plant Biology; 8; 5; 10-2006; 706-714
1435-8603
CONICET Digital
CONICET
url http://hdl.handle.net/11336/87118
identifier_str_mv Podazza, Griselda; Rosa, Mariana Daniela; González, Juan Antonio; Hilal, Mirna Beatriz; Prado, Fernando Eduardo; Cadmium induces changes in sucrose partitioning, invertase activities, and membrane functionality in roots of Rangpur Lime (Citrus limonia L. Osbeck) ; Wiley Blackwell Publishing, Inc; Plant Biology; 8; 5; 10-2006; 706-714
1435-8603
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1055/s-2006-924171
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1055/s-2006-924171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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