Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)

Autores
Godoy Pieri, Naira Caroline; Ramos da Silva Santos, Paulo; Santos Roballo, Kelly Cristine; Flamini, Mirta Alicia; Barbeito, Claudio Gustavo; Ambrosio, Carlos Eduardo; Miglino, Maria Angélica; do Santos Martins, Daniele
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study complements the previous investigations of the reproductive biology of maleviscachas, a rodent of a seasonal Hystricognathi that exhibits photoperiod-induced morpho-logical variations in the reproductive system. In the present study, a quantitative analysis ofspermatogenesis was performed during the summer and the spring. Spermatogonial cellswere analyzed to determine by immunolabelling for STRA8 and DAZL, which are essentialfor spermatogenesis. Six free-living male viscachas were captured, three animals in thesummer during the period of reproductive activity and three animals in the spring duringthe period of testicular regression. The testes of the viscachas were collected and processedfor light microscopy, macroscopic and immunochemical analyses. The germ and Sertolicells present in the seminiferous tubules were quantitatively analyzed in each animal. Theefficiency coefficient for spermatogonial mitosis, meiotic yield, overall spermatogenesisyield and Sertoli cell index, revealed that the Sertoli cells in male viscachas captured duringthe summer had a reduced capacity to structurally and nutritionally support the develop-ing round spermatids compared with the male viscachas captured during the spring. Theanimals produced less sperm during the spring than the summer, suggesting a seasonalimpact on spermatogenesis. Immunolabelling for STRA8 and DAZL was detected duringsummer and spring seasons. These results suggest that in seasonal rodents, such as themale viscachas, the photoperiod promotes significant changes in the testis and in the germcell yield.
Fil: Godoy Pieri, Naira Caroline. Universidade de Sao Paulo; Brasil
Fil: Ramos da Silva Santos, Paulo. Universidade de Sao Paulo; Brasil
Fil: Santos Roballo, Kelly Cristine. Universidade de Sao Paulo; Brasil
Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ambrosio, Carlos Eduardo. Universidade de Sao Paulo; Brasil
Fil: Miglino, Maria Angélica. Universidade de Sao Paulo; Brasil
Fil: do Santos Martins, Daniele. Universidade de Sao Paulo; Brasil
Materia
Rodent
Hystricomorpha
Reproduction Cycle
Photoperiod
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/79174
Acceder
id CONICETDig_9007f78e0f4c8d04bdd1a5a2b9ed4841
oai_identifier_str oai:ri.conicet.gov.ar:11336/79174
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)Godoy Pieri, Naira CarolineRamos da Silva Santos, PauloSantos Roballo, Kelly CristineFlamini, Mirta AliciaBarbeito, Claudio GustavoAmbrosio, Carlos EduardoMiglino, Maria Angélicado Santos Martins, DanieleRodentHystricomorphaReproduction CyclePhotoperiodhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4This study complements the previous investigations of the reproductive biology of maleviscachas, a rodent of a seasonal Hystricognathi that exhibits photoperiod-induced morpho-logical variations in the reproductive system. In the present study, a quantitative analysis ofspermatogenesis was performed during the summer and the spring. Spermatogonial cellswere analyzed to determine by immunolabelling for STRA8 and DAZL, which are essentialfor spermatogenesis. Six free-living male viscachas were captured, three animals in thesummer during the period of reproductive activity and three animals in the spring duringthe period of testicular regression. The testes of the viscachas were collected and processedfor light microscopy, macroscopic and immunochemical analyses. The germ and Sertolicells present in the seminiferous tubules were quantitatively analyzed in each animal. Theefficiency coefficient for spermatogonial mitosis, meiotic yield, overall spermatogenesisyield and Sertoli cell index, revealed that the Sertoli cells in male viscachas captured duringthe summer had a reduced capacity to structurally and nutritionally support the develop-ing round spermatids compared with the male viscachas captured during the spring. Theanimals produced less sperm during the spring than the summer, suggesting a seasonalimpact on spermatogenesis. Immunolabelling for STRA8 and DAZL was detected duringsummer and spring seasons. These results suggest that in seasonal rodents, such as themale viscachas, the photoperiod promotes significant changes in the testis and in the germcell yield.Fil: Godoy Pieri, Naira Caroline. Universidade de Sao Paulo; BrasilFil: Ramos da Silva Santos, Paulo. Universidade de Sao Paulo; BrasilFil: Santos Roballo, Kelly Cristine. Universidade de Sao Paulo; BrasilFil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Ambrosio, Carlos Eduardo. Universidade de Sao Paulo; BrasilFil: Miglino, Maria Angélica. Universidade de Sao Paulo; BrasilFil: do Santos Martins, Daniele. Universidade de Sao Paulo; BrasilElsevier Science2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/79174Godoy Pieri, Naira Caroline; Ramos da Silva Santos, Paulo; Santos Roballo, Kelly Cristine; Flamini, Mirta Alicia; Barbeito, Claudio Gustavo; et al.; Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus); Elsevier Science; Animal Reproduction Science; 149; 6-2014; 316-3240378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2014.07.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432014002139info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:15:38Zoai:ri.conicet.gov.ar:11336/79174instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:15:39.051CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
title Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
spellingShingle Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
Godoy Pieri, Naira Caroline
Rodent
Hystricomorpha
Reproduction Cycle
Photoperiod
title_short Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
title_full Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
title_fullStr Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
title_full_unstemmed Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
title_sort Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus)
dc.creator.none.fl_str_mv Godoy Pieri, Naira Caroline
Ramos da Silva Santos, Paulo
Santos Roballo, Kelly Cristine
Flamini, Mirta Alicia
Barbeito, Claudio Gustavo
Ambrosio, Carlos Eduardo
Miglino, Maria Angélica
do Santos Martins, Daniele
author Godoy Pieri, Naira Caroline
author_facet Godoy Pieri, Naira Caroline
Ramos da Silva Santos, Paulo
Santos Roballo, Kelly Cristine
Flamini, Mirta Alicia
Barbeito, Claudio Gustavo
Ambrosio, Carlos Eduardo
Miglino, Maria Angélica
do Santos Martins, Daniele
author_role author
author2 Ramos da Silva Santos, Paulo
Santos Roballo, Kelly Cristine
Flamini, Mirta Alicia
Barbeito, Claudio Gustavo
Ambrosio, Carlos Eduardo
Miglino, Maria Angélica
do Santos Martins, Daniele
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rodent
Hystricomorpha
Reproduction Cycle
Photoperiod
topic Rodent
Hystricomorpha
Reproduction Cycle
Photoperiod
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv This study complements the previous investigations of the reproductive biology of maleviscachas, a rodent of a seasonal Hystricognathi that exhibits photoperiod-induced morpho-logical variations in the reproductive system. In the present study, a quantitative analysis ofspermatogenesis was performed during the summer and the spring. Spermatogonial cellswere analyzed to determine by immunolabelling for STRA8 and DAZL, which are essentialfor spermatogenesis. Six free-living male viscachas were captured, three animals in thesummer during the period of reproductive activity and three animals in the spring duringthe period of testicular regression. The testes of the viscachas were collected and processedfor light microscopy, macroscopic and immunochemical analyses. The germ and Sertolicells present in the seminiferous tubules were quantitatively analyzed in each animal. Theefficiency coefficient for spermatogonial mitosis, meiotic yield, overall spermatogenesisyield and Sertoli cell index, revealed that the Sertoli cells in male viscachas captured duringthe summer had a reduced capacity to structurally and nutritionally support the develop-ing round spermatids compared with the male viscachas captured during the spring. Theanimals produced less sperm during the spring than the summer, suggesting a seasonalimpact on spermatogenesis. Immunolabelling for STRA8 and DAZL was detected duringsummer and spring seasons. These results suggest that in seasonal rodents, such as themale viscachas, the photoperiod promotes significant changes in the testis and in the germcell yield.
Fil: Godoy Pieri, Naira Caroline. Universidade de Sao Paulo; Brasil
Fil: Ramos da Silva Santos, Paulo. Universidade de Sao Paulo; Brasil
Fil: Santos Roballo, Kelly Cristine. Universidade de Sao Paulo; Brasil
Fil: Flamini, Mirta Alicia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Ciencias Básicas. Cátedra de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Ambrosio, Carlos Eduardo. Universidade de Sao Paulo; Brasil
Fil: Miglino, Maria Angélica. Universidade de Sao Paulo; Brasil
Fil: do Santos Martins, Daniele. Universidade de Sao Paulo; Brasil
description This study complements the previous investigations of the reproductive biology of maleviscachas, a rodent of a seasonal Hystricognathi that exhibits photoperiod-induced morpho-logical variations in the reproductive system. In the present study, a quantitative analysis ofspermatogenesis was performed during the summer and the spring. Spermatogonial cellswere analyzed to determine by immunolabelling for STRA8 and DAZL, which are essentialfor spermatogenesis. Six free-living male viscachas were captured, three animals in thesummer during the period of reproductive activity and three animals in the spring duringthe period of testicular regression. The testes of the viscachas were collected and processedfor light microscopy, macroscopic and immunochemical analyses. The germ and Sertolicells present in the seminiferous tubules were quantitatively analyzed in each animal. Theefficiency coefficient for spermatogonial mitosis, meiotic yield, overall spermatogenesisyield and Sertoli cell index, revealed that the Sertoli cells in male viscachas captured duringthe summer had a reduced capacity to structurally and nutritionally support the develop-ing round spermatids compared with the male viscachas captured during the spring. Theanimals produced less sperm during the spring than the summer, suggesting a seasonalimpact on spermatogenesis. Immunolabelling for STRA8 and DAZL was detected duringsummer and spring seasons. These results suggest that in seasonal rodents, such as themale viscachas, the photoperiod promotes significant changes in the testis and in the germcell yield.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/79174
Godoy Pieri, Naira Caroline; Ramos da Silva Santos, Paulo; Santos Roballo, Kelly Cristine; Flamini, Mirta Alicia; Barbeito, Claudio Gustavo; et al.; Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus); Elsevier Science; Animal Reproduction Science; 149; 6-2014; 316-324
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/79174
identifier_str_mv Godoy Pieri, Naira Caroline; Ramos da Silva Santos, Paulo; Santos Roballo, Kelly Cristine; Flamini, Mirta Alicia; Barbeito, Claudio Gustavo; et al.; Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus); Elsevier Science; Animal Reproduction Science; 149; 6-2014; 316-324
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2014.07.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432014002139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980845406650368
score 12.993085

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