Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America

Autores
Tempone, Antonio J.; de Souza Zezza Ramalho, Monique; Borely, Daniel; Pitaluga, André N.; Peçanha Brazil, Reginaldo; Brandão Filho, Sinval P.; Pessoa, Felipe A. C.; Bruno, Rafaela V.; Carvalho Costa, Filipe A.; Salomón, Oscar Daniel; Volf, Petr; Burleigh, Barbara A.; Aguiar, Eric R. G. R.; Traub Cseko, Yara M.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sandflies are known vectors of leishmaniasis. In the Old World, sandflies are also vectors of viruses while little is known about the capacity of New World insects to transmit viruses to humans. Here, we relate the identification of RNA sequences with homology to rhabdovirus nucleocapsids (NcPs) genes, initially in the Lutzomyia longipalpis LL5 cell lineage, named NcP1.1 and NcP2. The Rhabdoviridae family never retrotranscribes its RNA genome to DNA. The sequences here described were identified in cDNA and DNA from LL-5 cells and in adult insects indicating that they are transcribed endogenous viral elements (EVEs). The presence of NcP1.1 and NcP2 in the L. longipalpis genome was confirmed in silico. In addition to showing the genomic location of NcP1.1 and NcP2, we identified another rhabdoviral insertion named NcP1.2. Analysis of small RNA molecules derived from these sequences showed that NcP1.1 and NcP1.2 present a profile consistent with elements targeted by primary piRNAs, while NcP2 was restricted to the degradation profile. The presence of NcP1.1 and NcP2 was investigated in sandfly populations from South America and the Old World. These EVEs are shared by different sandfly populations in South America while none of the Old World species studied presented the insertions.
Fil: Tempone, Antonio J.. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;
Fil: de Souza Zezza Ramalho, Monique. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Borely, Daniel. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Pitaluga, André N.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Peçanha Brazil, Reginaldo. Instituto Oswaldo Cruz; Brasil
Fil: Brandão Filho, Sinval P.. Instituto Oswaldo Cruz; Brasil
Fil: Pessoa, Felipe A. C.. Instituto Oswaldo Cruz; Brasil
Fil: Bruno, Rafaela V.. Instituto Oswaldo Cruz; Brasil
Fil: Carvalho Costa, Filipe A.. Instituto Oswaldo Cruz; Brasil
Fil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Instituto Nacional de Medicina Tropical; Argentina
Fil: Volf, Petr. Charles University; República Checa
Fil: Burleigh, Barbara A.. Harvard Medical School; Estados Unidos
Fil: Aguiar, Eric R. G. R.. Universidade Federal de Minas Gerais; Brasil
Fil: Traub Cseko, Yara M.. Instituto Oswaldo Cruz; Brasil
Materia
Lutzomyia longipalpis;
endogenous viral element;
PIWI-RNA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/240296
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/240296
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South AmericaTempone, Antonio J.de Souza Zezza Ramalho, MoniqueBorely, DanielPitaluga, André N.Peçanha Brazil, ReginaldoBrandão Filho, Sinval P.Pessoa, Felipe A. C.Bruno, Rafaela V.Carvalho Costa, Filipe A.Salomón, Oscar DanielVolf, PetrBurleigh, Barbara A.Aguiar, Eric R. G. R.Traub Cseko, Yara M.Lutzomyia longipalpis;endogenous viral element;PIWI-RNAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sandflies are known vectors of leishmaniasis. In the Old World, sandflies are also vectors of viruses while little is known about the capacity of New World insects to transmit viruses to humans. Here, we relate the identification of RNA sequences with homology to rhabdovirus nucleocapsids (NcPs) genes, initially in the Lutzomyia longipalpis LL5 cell lineage, named NcP1.1 and NcP2. The Rhabdoviridae family never retrotranscribes its RNA genome to DNA. The sequences here described were identified in cDNA and DNA from LL-5 cells and in adult insects indicating that they are transcribed endogenous viral elements (EVEs). The presence of NcP1.1 and NcP2 in the L. longipalpis genome was confirmed in silico. In addition to showing the genomic location of NcP1.1 and NcP2, we identified another rhabdoviral insertion named NcP1.2. Analysis of small RNA molecules derived from these sequences showed that NcP1.1 and NcP1.2 present a profile consistent with elements targeted by primary piRNAs, while NcP2 was restricted to the degradation profile. The presence of NcP1.1 and NcP2 was investigated in sandfly populations from South America and the Old World. These EVEs are shared by different sandfly populations in South America while none of the Old World species studied presented the insertions.Fil: Tempone, Antonio J.. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;Fil: de Souza Zezza Ramalho, Monique. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;Fil: Borely, Daniel. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;Fil: Pitaluga, André N.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;Fil: Peçanha Brazil, Reginaldo. Instituto Oswaldo Cruz; BrasilFil: Brandão Filho, Sinval P.. Instituto Oswaldo Cruz; BrasilFil: Pessoa, Felipe A. C.. Instituto Oswaldo Cruz; BrasilFil: Bruno, Rafaela V.. Instituto Oswaldo Cruz; BrasilFil: Carvalho Costa, Filipe A.. Instituto Oswaldo Cruz; BrasilFil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Instituto Nacional de Medicina Tropical; ArgentinaFil: Volf, Petr. Charles University; República ChecaFil: Burleigh, Barbara A.. Harvard Medical School; Estados UnidosFil: Aguiar, Eric R. G. R.. Universidade Federal de Minas Gerais; BrasilFil: Traub Cseko, Yara M.. Instituto Oswaldo Cruz; BrasilMDPI2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/240296Tempone, Antonio J.; de Souza Zezza Ramalho, Monique; Borely, Daniel; Pitaluga, André N.; Peçanha Brazil, Reginaldo; et al.; Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America; MDPI; Viruses; 16; 3; 3-2024; 1-141999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/16/3/395info:eu-repo/semantics/altIdentifier/doi/10.3390/v16030395info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:37:01Zoai:ri.conicet.gov.ar:11336/240296instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:37:01.623CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
title Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
spellingShingle Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
Tempone, Antonio J.
Lutzomyia longipalpis;
endogenous viral element;
PIWI-RNA
title_short Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
title_full Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
title_fullStr Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
title_full_unstemmed Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
title_sort Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America
dc.creator.none.fl_str_mv Tempone, Antonio J.
de Souza Zezza Ramalho, Monique
Borely, Daniel
Pitaluga, André N.
Peçanha Brazil, Reginaldo
Brandão Filho, Sinval P.
Pessoa, Felipe A. C.
Bruno, Rafaela V.
Carvalho Costa, Filipe A.
Salomón, Oscar Daniel
Volf, Petr
Burleigh, Barbara A.
Aguiar, Eric R. G. R.
Traub Cseko, Yara M.
author Tempone, Antonio J.
author_facet Tempone, Antonio J.
de Souza Zezza Ramalho, Monique
Borely, Daniel
Pitaluga, André N.
Peçanha Brazil, Reginaldo
Brandão Filho, Sinval P.
Pessoa, Felipe A. C.
Bruno, Rafaela V.
Carvalho Costa, Filipe A.
Salomón, Oscar Daniel
Volf, Petr
Burleigh, Barbara A.
Aguiar, Eric R. G. R.
Traub Cseko, Yara M.
author_role author
author2 de Souza Zezza Ramalho, Monique
Borely, Daniel
Pitaluga, André N.
Peçanha Brazil, Reginaldo
Brandão Filho, Sinval P.
Pessoa, Felipe A. C.
Bruno, Rafaela V.
Carvalho Costa, Filipe A.
Salomón, Oscar Daniel
Volf, Petr
Burleigh, Barbara A.
Aguiar, Eric R. G. R.
Traub Cseko, Yara M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Lutzomyia longipalpis;
endogenous viral element;
PIWI-RNA
topic Lutzomyia longipalpis;
endogenous viral element;
PIWI-RNA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sandflies are known vectors of leishmaniasis. In the Old World, sandflies are also vectors of viruses while little is known about the capacity of New World insects to transmit viruses to humans. Here, we relate the identification of RNA sequences with homology to rhabdovirus nucleocapsids (NcPs) genes, initially in the Lutzomyia longipalpis LL5 cell lineage, named NcP1.1 and NcP2. The Rhabdoviridae family never retrotranscribes its RNA genome to DNA. The sequences here described were identified in cDNA and DNA from LL-5 cells and in adult insects indicating that they are transcribed endogenous viral elements (EVEs). The presence of NcP1.1 and NcP2 in the L. longipalpis genome was confirmed in silico. In addition to showing the genomic location of NcP1.1 and NcP2, we identified another rhabdoviral insertion named NcP1.2. Analysis of small RNA molecules derived from these sequences showed that NcP1.1 and NcP1.2 present a profile consistent with elements targeted by primary piRNAs, while NcP2 was restricted to the degradation profile. The presence of NcP1.1 and NcP2 was investigated in sandfly populations from South America and the Old World. These EVEs are shared by different sandfly populations in South America while none of the Old World species studied presented the insertions.
Fil: Tempone, Antonio J.. Ministerio de Salud de Brasil. Fundación Oswaldo Cruz. Instituto Oswaldo Cruz;
Fil: de Souza Zezza Ramalho, Monique. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Borely, Daniel. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Pitaluga, André N.. Instituto Oswaldo Cruz ; Fundacion Oswaldo Cruz ; Ministerio de Salud de Brasil;
Fil: Peçanha Brazil, Reginaldo. Instituto Oswaldo Cruz; Brasil
Fil: Brandão Filho, Sinval P.. Instituto Oswaldo Cruz; Brasil
Fil: Pessoa, Felipe A. C.. Instituto Oswaldo Cruz; Brasil
Fil: Bruno, Rafaela V.. Instituto Oswaldo Cruz; Brasil
Fil: Carvalho Costa, Filipe A.. Instituto Oswaldo Cruz; Brasil
Fil: Salomón, Oscar Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán". Instituto Nacional de Medicina Tropical; Argentina
Fil: Volf, Petr. Charles University; República Checa
Fil: Burleigh, Barbara A.. Harvard Medical School; Estados Unidos
Fil: Aguiar, Eric R. G. R.. Universidade Federal de Minas Gerais; Brasil
Fil: Traub Cseko, Yara M.. Instituto Oswaldo Cruz; Brasil
description Sandflies are known vectors of leishmaniasis. In the Old World, sandflies are also vectors of viruses while little is known about the capacity of New World insects to transmit viruses to humans. Here, we relate the identification of RNA sequences with homology to rhabdovirus nucleocapsids (NcPs) genes, initially in the Lutzomyia longipalpis LL5 cell lineage, named NcP1.1 and NcP2. The Rhabdoviridae family never retrotranscribes its RNA genome to DNA. The sequences here described were identified in cDNA and DNA from LL-5 cells and in adult insects indicating that they are transcribed endogenous viral elements (EVEs). The presence of NcP1.1 and NcP2 in the L. longipalpis genome was confirmed in silico. In addition to showing the genomic location of NcP1.1 and NcP2, we identified another rhabdoviral insertion named NcP1.2. Analysis of small RNA molecules derived from these sequences showed that NcP1.1 and NcP1.2 present a profile consistent with elements targeted by primary piRNAs, while NcP2 was restricted to the degradation profile. The presence of NcP1.1 and NcP2 was investigated in sandfly populations from South America and the Old World. These EVEs are shared by different sandfly populations in South America while none of the Old World species studied presented the insertions.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/240296
Tempone, Antonio J.; de Souza Zezza Ramalho, Monique; Borely, Daniel; Pitaluga, André N.; Peçanha Brazil, Reginaldo; et al.; Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America; MDPI; Viruses; 16; 3; 3-2024; 1-14
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/240296
identifier_str_mv Tempone, Antonio J.; de Souza Zezza Ramalho, Monique; Borely, Daniel; Pitaluga, André N.; Peçanha Brazil, Reginaldo; et al.; Rhabdoviral Endogenous Sequences Identified in the Leishmaniasis Vector Lutzomyia longipalpis Are Widespread in Sandflies from South America; MDPI; Viruses; 16; 3; 3-2024; 1-14
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/16/3/395
info:eu-repo/semantics/altIdentifier/doi/10.3390/v16030395
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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