Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats

Autores
Sanz Ressel, Berenice Liyare; Massone, Adriana Raquel; Barbeito, Claudio Gustavo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway may represent a potential target for pharmacological intervention in human and canine CSCC. Hypothesis/Objectives: The present study aimed to explore the expression pattern and status of activation of relevant signalling proteins of the PI3K/Akt/mTOR signalling pathway in feline CSCC. Methods and materials: The expression of pEGFRTyr1068, pAktSer473, pS6Ser235/236 combined with Ki-67, and the tumour suppressor protein PTEN was evaluated by immunohistochemical analysis in 45 samples of feline CSCC, using a tissue microarray. Results: The immunodetection using phosphospecific antibodies to detect the activated forms of signalling proteins showed that the PI3K/Akt/mTOR signalling pathway is frequently activated in feline CSCCs, and may be independent of the activation of EGFR. The results also showed that PTEN expression is not significantly altered in feline CSCCs. Conclusions and clinical importance: Our study shows that the persistent activation of the PI3K/Akt/mTOR signalling pathway represents a key event in feline CSCC, pointing to this signalling pathway being a potential therapeutic target in feline patients with CSCC.
Fil: Sanz Ressel, Berenice Liyare. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Massone, Adriana Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Materia
CAT
CSCC
MOLECULAR TARGETS
MTOR
SIGNALING MOLECULES
SKIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159452
Acceder
id CONICETDig_8f2ecf94fba150204777677d573de46b
oai_identifier_str oai:ri.conicet.gov.ar:11336/159452
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in catsSanz Ressel, Berenice LiyareMassone, Adriana RaquelBarbeito, Claudio GustavoCATCSCCMOLECULAR TARGETSMTORSIGNALING MOLECULESSKINhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway may represent a potential target for pharmacological intervention in human and canine CSCC. Hypothesis/Objectives: The present study aimed to explore the expression pattern and status of activation of relevant signalling proteins of the PI3K/Akt/mTOR signalling pathway in feline CSCC. Methods and materials: The expression of pEGFRTyr1068, pAktSer473, pS6Ser235/236 combined with Ki-67, and the tumour suppressor protein PTEN was evaluated by immunohistochemical analysis in 45 samples of feline CSCC, using a tissue microarray. Results: The immunodetection using phosphospecific antibodies to detect the activated forms of signalling proteins showed that the PI3K/Akt/mTOR signalling pathway is frequently activated in feline CSCCs, and may be independent of the activation of EGFR. The results also showed that PTEN expression is not significantly altered in feline CSCCs. Conclusions and clinical importance: Our study shows that the persistent activation of the PI3K/Akt/mTOR signalling pathway represents a key event in feline CSCC, pointing to this signalling pathway being a potential therapeutic target in feline patients with CSCC.Fil: Sanz Ressel, Berenice Liyare. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Massone, Adriana Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; ArgentinaFil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaWiley Blackwell Publishing, Inc2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159452Sanz Ressel, Berenice Liyare; Massone, Adriana Raquel; Barbeito, Claudio Gustavo; Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats; Wiley Blackwell Publishing, Inc; Veterinary Dermatology; 32; 6; 7-2021; 1-80959-4493CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/vde.13001info:eu-repo/semantics/altIdentifier/doi/10.1111/vde.13001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:21Zoai:ri.conicet.gov.ar:11336/159452instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:21.919CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
title Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
spellingShingle Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
Sanz Ressel, Berenice Liyare
CAT
CSCC
MOLECULAR TARGETS
MTOR
SIGNALING MOLECULES
SKIN
title_short Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
title_full Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
title_fullStr Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
title_full_unstemmed Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
title_sort Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats
dc.creator.none.fl_str_mv Sanz Ressel, Berenice Liyare
Massone, Adriana Raquel
Barbeito, Claudio Gustavo
author Sanz Ressel, Berenice Liyare
author_facet Sanz Ressel, Berenice Liyare
Massone, Adriana Raquel
Barbeito, Claudio Gustavo
author_role author
author2 Massone, Adriana Raquel
Barbeito, Claudio Gustavo
author2_role author
author
dc.subject.none.fl_str_mv CAT
CSCC
MOLECULAR TARGETS
MTOR
SIGNALING MOLECULES
SKIN
topic CAT
CSCC
MOLECULAR TARGETS
MTOR
SIGNALING MOLECULES
SKIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway may represent a potential target for pharmacological intervention in human and canine CSCC. Hypothesis/Objectives: The present study aimed to explore the expression pattern and status of activation of relevant signalling proteins of the PI3K/Akt/mTOR signalling pathway in feline CSCC. Methods and materials: The expression of pEGFRTyr1068, pAktSer473, pS6Ser235/236 combined with Ki-67, and the tumour suppressor protein PTEN was evaluated by immunohistochemical analysis in 45 samples of feline CSCC, using a tissue microarray. Results: The immunodetection using phosphospecific antibodies to detect the activated forms of signalling proteins showed that the PI3K/Akt/mTOR signalling pathway is frequently activated in feline CSCCs, and may be independent of the activation of EGFR. The results also showed that PTEN expression is not significantly altered in feline CSCCs. Conclusions and clinical importance: Our study shows that the persistent activation of the PI3K/Akt/mTOR signalling pathway represents a key event in feline CSCC, pointing to this signalling pathway being a potential therapeutic target in feline patients with CSCC.
Fil: Sanz Ressel, Berenice Liyare. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Massone, Adriana Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Laboratorio de Patología Especial Veterinaria "Dr. Bernardo Epstein"; Argentina
Fil: Barbeito, Claudio Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
description Background: Cutaneous squamous cell carcinoma (CSCC) represents the most common malignant tumour of the feline skin. Emerging evidence suggests that the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) signalling pathway may represent a potential target for pharmacological intervention in human and canine CSCC. Hypothesis/Objectives: The present study aimed to explore the expression pattern and status of activation of relevant signalling proteins of the PI3K/Akt/mTOR signalling pathway in feline CSCC. Methods and materials: The expression of pEGFRTyr1068, pAktSer473, pS6Ser235/236 combined with Ki-67, and the tumour suppressor protein PTEN was evaluated by immunohistochemical analysis in 45 samples of feline CSCC, using a tissue microarray. Results: The immunodetection using phosphospecific antibodies to detect the activated forms of signalling proteins showed that the PI3K/Akt/mTOR signalling pathway is frequently activated in feline CSCCs, and may be independent of the activation of EGFR. The results also showed that PTEN expression is not significantly altered in feline CSCCs. Conclusions and clinical importance: Our study shows that the persistent activation of the PI3K/Akt/mTOR signalling pathway represents a key event in feline CSCC, pointing to this signalling pathway being a potential therapeutic target in feline patients with CSCC.
publishDate 2021
dc.date.none.fl_str_mv 2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159452
Sanz Ressel, Berenice Liyare; Massone, Adriana Raquel; Barbeito, Claudio Gustavo; Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats; Wiley Blackwell Publishing, Inc; Veterinary Dermatology; 32; 6; 7-2021; 1-8
0959-4493
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159452
identifier_str_mv Sanz Ressel, Berenice Liyare; Massone, Adriana Raquel; Barbeito, Claudio Gustavo; Persistent activation of the mammalian target of rapamycin signaling pathway in cutaneous squamous cell carcinomas in cats; Wiley Blackwell Publishing, Inc; Veterinary Dermatology; 32; 6; 7-2021; 1-8
0959-4493
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/vde.13001
info:eu-repo/semantics/altIdentifier/doi/10.1111/vde.13001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082862060666880
score 13.22299

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