Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization

Autores
Gonzalez, Claudio Fabricio; Fariña, Julia Ines; Castellanos, Lucia Ines
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Different nutritional and operative conditions for improving amylolytic enzyme production by the amylolytic yeast Saccharomycopsis fibuligera DSM-70554 were evaluated. Under selected conditions, cassava starch could be efficiently utilized. Culture conditions such as oxygen saturation (50% dissolved oxygen tension), temperature (34 °C) and pH (5.5) showed a significant influence on α-amylase and glucoamylase production. An optimized culture medium (OPT) formulated by the chemostat pulse-shift method, containing yeast extract as the most favorable organic nitrogen source, vitamins and certain salts (MgCl2, MnSO4, CaCl2) allowed to achieve the highest amylolytic enzyme production, thus leading to an optimal liquefaction and saccharification of cassava starch. Tween 80 (0.2–1% w/v) demonstrated to be a favorable amendment for amylolytic enzyme production. Under the conditions herein described, a 97% degradation of cassava starch could be finally attained under batch culture mode. Maximal values of 633.3 U L−1 h−1 of α-amylase and 72.1 U L−1 h−1 of glucoamylase under optimized conditions represented ∼9 and ∼3-fold increments, respectively, as compared to preliminary fermentation assays under non-optimal conditions.
Fil: Gonzalez, Claudio Fabricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Saccharomycopsis Fibuligera
Α-Amylase
Glucoamylase
Fermentation
Cassava Starch
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/43459
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/43459
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilizationGonzalez, Claudio FabricioFariña, Julia InesCastellanos, Lucia InesSaccharomycopsis FibuligeraΑ-AmylaseGlucoamylaseFermentationCassava Starchhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Different nutritional and operative conditions for improving amylolytic enzyme production by the amylolytic yeast Saccharomycopsis fibuligera DSM-70554 were evaluated. Under selected conditions, cassava starch could be efficiently utilized. Culture conditions such as oxygen saturation (50% dissolved oxygen tension), temperature (34 °C) and pH (5.5) showed a significant influence on α-amylase and glucoamylase production. An optimized culture medium (OPT) formulated by the chemostat pulse-shift method, containing yeast extract as the most favorable organic nitrogen source, vitamins and certain salts (MgCl2, MnSO4, CaCl2) allowed to achieve the highest amylolytic enzyme production, thus leading to an optimal liquefaction and saccharification of cassava starch. Tween 80 (0.2–1% w/v) demonstrated to be a favorable amendment for amylolytic enzyme production. Under the conditions herein described, a 97% degradation of cassava starch could be finally attained under batch culture mode. Maximal values of 633.3 U L−1 h−1 of α-amylase and 72.1 U L−1 h−1 of glucoamylase under optimized conditions represented ∼9 and ∼3-fold increments, respectively, as compared to preliminary fermentation assays under non-optimal conditions.Fil: Gonzalez, Claudio Fabricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier Science Inc2008-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/43459Gonzalez, Claudio Fabricio; Fariña, Julia Ines; Castellanos, Lucia Ines; Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization; Elsevier Science Inc; Enzyme and Microbial Technology; 42; 3; 2-2008; 272-2770141-0229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2007.10.005info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022907003341info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:11:31Zoai:ri.conicet.gov.ar:11336/43459instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:11:31.469CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
title Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
spellingShingle Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
Gonzalez, Claudio Fabricio
Saccharomycopsis Fibuligera
Α-Amylase
Glucoamylase
Fermentation
Cassava Starch
title_short Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
title_full Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
title_fullStr Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
title_full_unstemmed Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
title_sort Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization
dc.creator.none.fl_str_mv Gonzalez, Claudio Fabricio
Fariña, Julia Ines
Castellanos, Lucia Ines
author Gonzalez, Claudio Fabricio
author_facet Gonzalez, Claudio Fabricio
Fariña, Julia Ines
Castellanos, Lucia Ines
author_role author
author2 Fariña, Julia Ines
Castellanos, Lucia Ines
author2_role author
author
dc.subject.none.fl_str_mv Saccharomycopsis Fibuligera
Α-Amylase
Glucoamylase
Fermentation
Cassava Starch
topic Saccharomycopsis Fibuligera
Α-Amylase
Glucoamylase
Fermentation
Cassava Starch
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Different nutritional and operative conditions for improving amylolytic enzyme production by the amylolytic yeast Saccharomycopsis fibuligera DSM-70554 were evaluated. Under selected conditions, cassava starch could be efficiently utilized. Culture conditions such as oxygen saturation (50% dissolved oxygen tension), temperature (34 °C) and pH (5.5) showed a significant influence on α-amylase and glucoamylase production. An optimized culture medium (OPT) formulated by the chemostat pulse-shift method, containing yeast extract as the most favorable organic nitrogen source, vitamins and certain salts (MgCl2, MnSO4, CaCl2) allowed to achieve the highest amylolytic enzyme production, thus leading to an optimal liquefaction and saccharification of cassava starch. Tween 80 (0.2–1% w/v) demonstrated to be a favorable amendment for amylolytic enzyme production. Under the conditions herein described, a 97% degradation of cassava starch could be finally attained under batch culture mode. Maximal values of 633.3 U L−1 h−1 of α-amylase and 72.1 U L−1 h−1 of glucoamylase under optimized conditions represented ∼9 and ∼3-fold increments, respectively, as compared to preliminary fermentation assays under non-optimal conditions.
Fil: Gonzalez, Claudio Fabricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Different nutritional and operative conditions for improving amylolytic enzyme production by the amylolytic yeast Saccharomycopsis fibuligera DSM-70554 were evaluated. Under selected conditions, cassava starch could be efficiently utilized. Culture conditions such as oxygen saturation (50% dissolved oxygen tension), temperature (34 °C) and pH (5.5) showed a significant influence on α-amylase and glucoamylase production. An optimized culture medium (OPT) formulated by the chemostat pulse-shift method, containing yeast extract as the most favorable organic nitrogen source, vitamins and certain salts (MgCl2, MnSO4, CaCl2) allowed to achieve the highest amylolytic enzyme production, thus leading to an optimal liquefaction and saccharification of cassava starch. Tween 80 (0.2–1% w/v) demonstrated to be a favorable amendment for amylolytic enzyme production. Under the conditions herein described, a 97% degradation of cassava starch could be finally attained under batch culture mode. Maximal values of 633.3 U L−1 h−1 of α-amylase and 72.1 U L−1 h−1 of glucoamylase under optimized conditions represented ∼9 and ∼3-fold increments, respectively, as compared to preliminary fermentation assays under non-optimal conditions.
publishDate 2008
dc.date.none.fl_str_mv 2008-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/43459
Gonzalez, Claudio Fabricio; Fariña, Julia Ines; Castellanos, Lucia Ines; Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization; Elsevier Science Inc; Enzyme and Microbial Technology; 42; 3; 2-2008; 272-277
0141-0229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/43459
identifier_str_mv Gonzalez, Claudio Fabricio; Fariña, Julia Ines; Castellanos, Lucia Ines; Optimized amylolytic enzymes production in Saccharomycopsis fibuligera DSM-70554: An approach to efficient cassava starch utilization; Elsevier Science Inc; Enzyme and Microbial Technology; 42; 3; 2-2008; 272-277
0141-0229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.enzmictec.2007.10.005
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0141022907003341
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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