Mitochondrial function in antarctic nototheniids with ND6 translocation

Autores
Mark, Felix C.; Lucassen, Magnus; Strobel, Anneli; Barrera Oro, Esteban; Koschnick, Nils; Zane, Lorenzo; Patarnello, Tomaso; Pörtner, Hans O.; Papetti, Chiara
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fish of the suborder Notothenioidei have successfully radiated into the Southern Ocean and today comprise the dominant fish sub-order in Antarctic waters in terms of biomass and species abundance. During evolution in the cold and stable Antarctic climate, the Antarctic lineage of notothenioids developed several unique physiological adaptations, which make them extremely vulnerable to the rapid warming of Antarctic waters currently observed. Only recently, a further phenomenon exclusive to notothenioid fish was reported: the translocation of the mitochondrial gene encoding the NADH Dehydrogenase subunit 6 (ND6), an indispensable part of complex I in the mitochondrial electron transport system. This study investigated the potential physiological consequences of ND6 translocation for the function and thermal sensitivity of the electron transport system in isolated liver mitochondria of the two nototheniid species Notothenia coriiceps and Notothenia rossii, with special attention to the contributions of complex I (NADH DH) and complex II (Succinate DH) to oxidative phosphorylation. Furthermore, enzymatic activities of NADH:Cytochrome c Oxidoreductase and Cytochrome C Oxidase were measured in membrane-enriched tissue extracts. During acute thermal challenge (0-15°C), capacities of mitochondrial respiration and enzymatic function in the liver could only be increased until 9°C. Mitochondrial complex I (NADH Dehydrogenase) was fully functional but displayed a higher thermal sensitivity than the other complexes of the electron transport system, which may specifically result from its unique amino acid composition, revealing a lower degree of stability in notothenioids in general. We interpret the translocation of ND6 as functionally neutral but the change in amino acid sequence as adaptive and supportive of cold stenothermy in Antarctic nototheniids. From these findings, an enhanced sensitivity to ocean warming can be deduced for Antarctic notothenioid fish.
Fil: Mark, Felix C.. Alfred Wegener Institut; Alemania
Fil: Lucassen, Magnus. Alfred Wegener Institut; Alemania
Fil: Strobel, Anneli. Alfred Wegener Institut; Alemania
Fil: Barrera Oro, Esteban. Ministerio de Relaciones Exteriores, Comercio Internacional y Culto. Direccion Nacional del Antártico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Koschnick, Nils. No especifíca;
Fil: Zane, Lorenzo. Università di Padova; Italia
Fil: Patarnello, Tomaso. Università di Padova; Italia
Fil: Pörtner, Hans O.. No especifíca;
Fil: Papetti, Chiara. Università di Padova; Italia
Materia
Aerobic energy metabolism
Notothenia rossii
Notothenia coriiceps
mitochondria
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/194850
Acceder
id CONICETDig_8ea4982261685e32371da56dc51f8bcf
oai_identifier_str oai:ri.conicet.gov.ar:11336/194850
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mitochondrial function in antarctic nototheniids with ND6 translocationMark, Felix C.Lucassen, MagnusStrobel, AnneliBarrera Oro, EstebanKoschnick, NilsZane, LorenzoPatarnello, TomasoPörtner, Hans O.Papetti, ChiaraAerobic energy metabolismNotothenia rossiiNotothenia coriicepsmitochondriahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fish of the suborder Notothenioidei have successfully radiated into the Southern Ocean and today comprise the dominant fish sub-order in Antarctic waters in terms of biomass and species abundance. During evolution in the cold and stable Antarctic climate, the Antarctic lineage of notothenioids developed several unique physiological adaptations, which make them extremely vulnerable to the rapid warming of Antarctic waters currently observed. Only recently, a further phenomenon exclusive to notothenioid fish was reported: the translocation of the mitochondrial gene encoding the NADH Dehydrogenase subunit 6 (ND6), an indispensable part of complex I in the mitochondrial electron transport system. This study investigated the potential physiological consequences of ND6 translocation for the function and thermal sensitivity of the electron transport system in isolated liver mitochondria of the two nototheniid species Notothenia coriiceps and Notothenia rossii, with special attention to the contributions of complex I (NADH DH) and complex II (Succinate DH) to oxidative phosphorylation. Furthermore, enzymatic activities of NADH:Cytochrome c Oxidoreductase and Cytochrome C Oxidase were measured in membrane-enriched tissue extracts. During acute thermal challenge (0-15°C), capacities of mitochondrial respiration and enzymatic function in the liver could only be increased until 9°C. Mitochondrial complex I (NADH Dehydrogenase) was fully functional but displayed a higher thermal sensitivity than the other complexes of the electron transport system, which may specifically result from its unique amino acid composition, revealing a lower degree of stability in notothenioids in general. We interpret the translocation of ND6 as functionally neutral but the change in amino acid sequence as adaptive and supportive of cold stenothermy in Antarctic nototheniids. From these findings, an enhanced sensitivity to ocean warming can be deduced for Antarctic notothenioid fish.Fil: Mark, Felix C.. Alfred Wegener Institut; AlemaniaFil: Lucassen, Magnus. Alfred Wegener Institut; AlemaniaFil: Strobel, Anneli. Alfred Wegener Institut; AlemaniaFil: Barrera Oro, Esteban. Ministerio de Relaciones Exteriores, Comercio Internacional y Culto. Direccion Nacional del Antártico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Koschnick, Nils. No especifíca;Fil: Zane, Lorenzo. Università di Padova; ItaliaFil: Patarnello, Tomaso. Università di Padova; ItaliaFil: Pörtner, Hans O.. No especifíca;Fil: Papetti, Chiara. Università di Padova; ItaliaPublic Library of Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/194850Mark, Felix C.; Lucassen, Magnus; Strobel, Anneli; Barrera Oro, Esteban; Koschnick, Nils; et al.; Mitochondrial function in antarctic nototheniids with ND6 translocation; Public Library of Science; Plos One; 7; 2; 2-2012; 1-121932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0031860info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0031860info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:36:00Zoai:ri.conicet.gov.ar:11336/194850instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:36:01.076CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mitochondrial function in antarctic nototheniids with ND6 translocation
title Mitochondrial function in antarctic nototheniids with ND6 translocation
spellingShingle Mitochondrial function in antarctic nototheniids with ND6 translocation
Mark, Felix C.
Aerobic energy metabolism
Notothenia rossii
Notothenia coriiceps
mitochondria
title_short Mitochondrial function in antarctic nototheniids with ND6 translocation
title_full Mitochondrial function in antarctic nototheniids with ND6 translocation
title_fullStr Mitochondrial function in antarctic nototheniids with ND6 translocation
title_full_unstemmed Mitochondrial function in antarctic nototheniids with ND6 translocation
title_sort Mitochondrial function in antarctic nototheniids with ND6 translocation
dc.creator.none.fl_str_mv Mark, Felix C.
Lucassen, Magnus
Strobel, Anneli
Barrera Oro, Esteban
Koschnick, Nils
Zane, Lorenzo
Patarnello, Tomaso
Pörtner, Hans O.
Papetti, Chiara
author Mark, Felix C.
author_facet Mark, Felix C.
Lucassen, Magnus
Strobel, Anneli
Barrera Oro, Esteban
Koschnick, Nils
Zane, Lorenzo
Patarnello, Tomaso
Pörtner, Hans O.
Papetti, Chiara
author_role author
author2 Lucassen, Magnus
Strobel, Anneli
Barrera Oro, Esteban
Koschnick, Nils
Zane, Lorenzo
Patarnello, Tomaso
Pörtner, Hans O.
Papetti, Chiara
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aerobic energy metabolism
Notothenia rossii
Notothenia coriiceps
mitochondria
topic Aerobic energy metabolism
Notothenia rossii
Notothenia coriiceps
mitochondria
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fish of the suborder Notothenioidei have successfully radiated into the Southern Ocean and today comprise the dominant fish sub-order in Antarctic waters in terms of biomass and species abundance. During evolution in the cold and stable Antarctic climate, the Antarctic lineage of notothenioids developed several unique physiological adaptations, which make them extremely vulnerable to the rapid warming of Antarctic waters currently observed. Only recently, a further phenomenon exclusive to notothenioid fish was reported: the translocation of the mitochondrial gene encoding the NADH Dehydrogenase subunit 6 (ND6), an indispensable part of complex I in the mitochondrial electron transport system. This study investigated the potential physiological consequences of ND6 translocation for the function and thermal sensitivity of the electron transport system in isolated liver mitochondria of the two nototheniid species Notothenia coriiceps and Notothenia rossii, with special attention to the contributions of complex I (NADH DH) and complex II (Succinate DH) to oxidative phosphorylation. Furthermore, enzymatic activities of NADH:Cytochrome c Oxidoreductase and Cytochrome C Oxidase were measured in membrane-enriched tissue extracts. During acute thermal challenge (0-15°C), capacities of mitochondrial respiration and enzymatic function in the liver could only be increased until 9°C. Mitochondrial complex I (NADH Dehydrogenase) was fully functional but displayed a higher thermal sensitivity than the other complexes of the electron transport system, which may specifically result from its unique amino acid composition, revealing a lower degree of stability in notothenioids in general. We interpret the translocation of ND6 as functionally neutral but the change in amino acid sequence as adaptive and supportive of cold stenothermy in Antarctic nototheniids. From these findings, an enhanced sensitivity to ocean warming can be deduced for Antarctic notothenioid fish.
Fil: Mark, Felix C.. Alfred Wegener Institut; Alemania
Fil: Lucassen, Magnus. Alfred Wegener Institut; Alemania
Fil: Strobel, Anneli. Alfred Wegener Institut; Alemania
Fil: Barrera Oro, Esteban. Ministerio de Relaciones Exteriores, Comercio Internacional y Culto. Direccion Nacional del Antártico; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Koschnick, Nils. No especifíca;
Fil: Zane, Lorenzo. Università di Padova; Italia
Fil: Patarnello, Tomaso. Università di Padova; Italia
Fil: Pörtner, Hans O.. No especifíca;
Fil: Papetti, Chiara. Università di Padova; Italia
description Fish of the suborder Notothenioidei have successfully radiated into the Southern Ocean and today comprise the dominant fish sub-order in Antarctic waters in terms of biomass and species abundance. During evolution in the cold and stable Antarctic climate, the Antarctic lineage of notothenioids developed several unique physiological adaptations, which make them extremely vulnerable to the rapid warming of Antarctic waters currently observed. Only recently, a further phenomenon exclusive to notothenioid fish was reported: the translocation of the mitochondrial gene encoding the NADH Dehydrogenase subunit 6 (ND6), an indispensable part of complex I in the mitochondrial electron transport system. This study investigated the potential physiological consequences of ND6 translocation for the function and thermal sensitivity of the electron transport system in isolated liver mitochondria of the two nototheniid species Notothenia coriiceps and Notothenia rossii, with special attention to the contributions of complex I (NADH DH) and complex II (Succinate DH) to oxidative phosphorylation. Furthermore, enzymatic activities of NADH:Cytochrome c Oxidoreductase and Cytochrome C Oxidase were measured in membrane-enriched tissue extracts. During acute thermal challenge (0-15°C), capacities of mitochondrial respiration and enzymatic function in the liver could only be increased until 9°C. Mitochondrial complex I (NADH Dehydrogenase) was fully functional but displayed a higher thermal sensitivity than the other complexes of the electron transport system, which may specifically result from its unique amino acid composition, revealing a lower degree of stability in notothenioids in general. We interpret the translocation of ND6 as functionally neutral but the change in amino acid sequence as adaptive and supportive of cold stenothermy in Antarctic nototheniids. From these findings, an enhanced sensitivity to ocean warming can be deduced for Antarctic notothenioid fish.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/194850
Mark, Felix C.; Lucassen, Magnus; Strobel, Anneli; Barrera Oro, Esteban; Koschnick, Nils; et al.; Mitochondrial function in antarctic nototheniids with ND6 translocation; Public Library of Science; Plos One; 7; 2; 2-2012; 1-12
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/194850
identifier_str_mv Mark, Felix C.; Lucassen, Magnus; Strobel, Anneli; Barrera Oro, Esteban; Koschnick, Nils; et al.; Mitochondrial function in antarctic nototheniids with ND6 translocation; Public Library of Science; Plos One; 7; 2; 2-2012; 1-12
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0031860
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0031860
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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