Novel red fluorophores with superior performance in STED microscopy
- Autores
- Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; Schill, Heiko; Berning, Sebastian; Jakobs, Stefan; Donnert, Gerald; Belov, Vladimir N.; Hell, Stefan W.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
Fil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
Fil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania - Materia
-
Bioconjugation
Confocal
Fluorescence
Microscopy
Nanoscopy
Rhodamines
Sted
Stimulated Emission Depletion
Super-Resolution - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/67724
Ver los metadatos del registro completo
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Novel red fluorophores with superior performance in STED microscopyWurm, Christian A.Kolmakov, KirillGöttfert, FabianTa, HaisenBossi, Mariano LuisSchill, HeikoBerning, SebastianJakobs, StefanDonnert, GeraldBelov, Vladimir N.Hell, Stefan W.BioconjugationConfocalFluorescenceMicroscopyNanoscopyRhodaminesStedStimulated Emission DepletionSuper-Resolutionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; AlemaniaFil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; AlemaniaFil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; AlemaniaSpringer Open2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67724Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-72192-2853CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/2192-2853-1-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1186/2192-2853-1-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:08Zoai:ri.conicet.gov.ar:11336/67724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:08.565CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Novel red fluorophores with superior performance in STED microscopy |
title |
Novel red fluorophores with superior performance in STED microscopy |
spellingShingle |
Novel red fluorophores with superior performance in STED microscopy Wurm, Christian A. Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines Sted Stimulated Emission Depletion Super-Resolution |
title_short |
Novel red fluorophores with superior performance in STED microscopy |
title_full |
Novel red fluorophores with superior performance in STED microscopy |
title_fullStr |
Novel red fluorophores with superior performance in STED microscopy |
title_full_unstemmed |
Novel red fluorophores with superior performance in STED microscopy |
title_sort |
Novel red fluorophores with superior performance in STED microscopy |
dc.creator.none.fl_str_mv |
Wurm, Christian A. Kolmakov, Kirill Göttfert, Fabian Ta, Haisen Bossi, Mariano Luis Schill, Heiko Berning, Sebastian Jakobs, Stefan Donnert, Gerald Belov, Vladimir N. Hell, Stefan W. |
author |
Wurm, Christian A. |
author_facet |
Wurm, Christian A. Kolmakov, Kirill Göttfert, Fabian Ta, Haisen Bossi, Mariano Luis Schill, Heiko Berning, Sebastian Jakobs, Stefan Donnert, Gerald Belov, Vladimir N. Hell, Stefan W. |
author_role |
author |
author2 |
Kolmakov, Kirill Göttfert, Fabian Ta, Haisen Bossi, Mariano Luis Schill, Heiko Berning, Sebastian Jakobs, Stefan Donnert, Gerald Belov, Vladimir N. Hell, Stefan W. |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines Sted Stimulated Emission Depletion Super-Resolution |
topic |
Bioconjugation Confocal Fluorescence Microscopy Nanoscopy Rhodamines Sted Stimulated Emission Depletion Super-Resolution |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al. Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania Fil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina Fil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania Fil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania |
description |
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/67724 Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-7 2192-2853 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/67724 |
identifier_str_mv |
Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-7 2192-2853 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1186/2192-2853-1-7 info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1186/2192-2853-1-7 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer Open |
publisher.none.fl_str_mv |
Springer Open |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842270032814407680 |
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13.13397 |