Novel red fluorophores with superior performance in STED microscopy

Autores
Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; Schill, Heiko; Berning, Sebastian; Jakobs, Stefan; Donnert, Gerald; Belov, Vladimir N.; Hell, Stefan W.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
Fil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
Fil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
Materia
Bioconjugation
Confocal
Fluorescence
Microscopy
Nanoscopy
Rhodamines
Sted
Stimulated Emission Depletion
Super-Resolution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/67724
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel red fluorophores with superior performance in STED microscopyWurm, Christian A.Kolmakov, KirillGöttfert, FabianTa, HaisenBossi, Mariano LuisSchill, HeikoBerning, SebastianJakobs, StefanDonnert, GeraldBelov, Vladimir N.Hell, Stefan W.BioconjugationConfocalFluorescenceMicroscopyNanoscopyRhodaminesStedStimulated Emission DepletionSuper-Resolutionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; AlemaniaFil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; AlemaniaFil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; AlemaniaFil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; AlemaniaSpringer Open2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/67724Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-72192-2853CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1186/2192-2853-1-7info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1186/2192-2853-1-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:54Zoai:ri.conicet.gov.ar:11336/67724instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:54.583CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel red fluorophores with superior performance in STED microscopy
title Novel red fluorophores with superior performance in STED microscopy
spellingShingle Novel red fluorophores with superior performance in STED microscopy
Wurm, Christian A.
Bioconjugation
Confocal
Fluorescence
Microscopy
Nanoscopy
Rhodamines
Sted
Stimulated Emission Depletion
Super-Resolution
title_short Novel red fluorophores with superior performance in STED microscopy
title_full Novel red fluorophores with superior performance in STED microscopy
title_fullStr Novel red fluorophores with superior performance in STED microscopy
title_full_unstemmed Novel red fluorophores with superior performance in STED microscopy
title_sort Novel red fluorophores with superior performance in STED microscopy
dc.creator.none.fl_str_mv Wurm, Christian A.
Kolmakov, Kirill
Göttfert, Fabian
Ta, Haisen
Bossi, Mariano Luis
Schill, Heiko
Berning, Sebastian
Jakobs, Stefan
Donnert, Gerald
Belov, Vladimir N.
Hell, Stefan W.
author Wurm, Christian A.
author_facet Wurm, Christian A.
Kolmakov, Kirill
Göttfert, Fabian
Ta, Haisen
Bossi, Mariano Luis
Schill, Heiko
Berning, Sebastian
Jakobs, Stefan
Donnert, Gerald
Belov, Vladimir N.
Hell, Stefan W.
author_role author
author2 Kolmakov, Kirill
Göttfert, Fabian
Ta, Haisen
Bossi, Mariano Luis
Schill, Heiko
Berning, Sebastian
Jakobs, Stefan
Donnert, Gerald
Belov, Vladimir N.
Hell, Stefan W.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bioconjugation
Confocal
Fluorescence
Microscopy
Nanoscopy
Rhodamines
Sted
Stimulated Emission Depletion
Super-Resolution
topic Bioconjugation
Confocal
Fluorescence
Microscopy
Nanoscopy
Rhodamines
Sted
Stimulated Emission Depletion
Super-Resolution
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
Fil: Wurm, Christian A.. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
Fil: Kolmakov, Kirill. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Göttfert, Fabian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Ta, Haisen. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Bossi, Mariano Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Schill, Heiko. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Berning, Sebastian. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Jakobs, Stefan. Max Planck Institute for Biophysical Chemistry; Alemania. Georg-August University of Göttingen Medical School; Alemania
Fil: Donnert, Gerald. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Belov, Vladimir N.. Max Planck Institute for Biophysical Chemistry; Alemania
Fil: Hell, Stefan W.. Max Planck Institute for Biophysical Chemistry; Alemania
description In optical microscopy, most red-emitting dyes provide only moderate performance due to unspecific binding, poor labeling efficiency, and insufficient brightness. Here we report on four novel red fluororescent dyes, including the first phosphorylated dye, created by combining a rigidized rhodamine backbone with various polar groups. They exhibit large fluorescence quantum yields and improved NHS ester stability. While these fluorophores are highly suitable for fluorescence microscopy in general, they excel in stimulated emission depletion (STED) microscopy, providing < 25 nm spatial resolution in raw images of cells. © 2012 Wurm et al.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/67724
Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-7
2192-2853
CONICET Digital
CONICET
url http://hdl.handle.net/11336/67724
identifier_str_mv Wurm, Christian A.; Kolmakov, Kirill; Göttfert, Fabian; Ta, Haisen; Bossi, Mariano Luis; et al.; Novel red fluorophores with superior performance in STED microscopy; Springer Open; Optical Nanoscopy; 1; 1; 9-2012; 1-7
2192-2853
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/2192-2853-1-7
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1186/2192-2853-1-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Open
publisher.none.fl_str_mv Springer Open
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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