Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation

Autores
Stagnoli, Antonela Soledad; Luna, Maria Alejandra; Villa, Cristian C.; Alustiza, Fabrisio Eduardo; Niebylski, Ana Maria; Moyano, Fernando; Correa, Nestor Mariano; Falcone, Ruben Dario
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We evaluate in vitro and in vivo toxicity and stability in an acidic environment of new vesicles formed by the catanionic surfactant bis(2-ethylhexyl) sulfosuccinate benzyl-n-hexadecyldimethylammonium (AOT-BHD) in order to investigate their potential application as an oral drug delivery system. Unilamellar vesicles were spontaneously formed by dissolving AOT-BHD in water and their toxicity was evaluated through in vitro and in vivo assays. Cell membrane permeability assays (hemolytic activity, Trypan blue assay) and cellular survival or proliferation (MTT assay) were performed. The results showed that only the highest concentration of vesicles tested (2 mg mL-1) diminished the red blood cells' resistance. In vivo toxicity evaluation was carried out on mice through lethal dose 50 (LD50) experiments. The safety for living organisms in doses lower than 0.05 mg mL-1 and the acid pH stability makes our AOT-BHD vesicles a very promising candidate for oral drug delivery.
Fil: Stagnoli, Antonela Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Luna, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Villa, Cristian C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Niebylski, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Moyano, Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Correa, Nestor Mariano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
CATANIONIC
AOT-BHD
VESICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61519
Acceder
id CONICETDig_8d8bfeddef4d63ff6695827ca766eda3
oai_identifier_str oai:ri.conicet.gov.ar:11336/61519
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluationStagnoli, Antonela SoledadLuna, Maria AlejandraVilla, Cristian C.Alustiza, Fabrisio EduardoNiebylski, Ana MariaMoyano, FernandoCorrea, Nestor MarianoFalcone, Ruben DarioCATANIONICAOT-BHDVESICLEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1We evaluate in vitro and in vivo toxicity and stability in an acidic environment of new vesicles formed by the catanionic surfactant bis(2-ethylhexyl) sulfosuccinate benzyl-n-hexadecyldimethylammonium (AOT-BHD) in order to investigate their potential application as an oral drug delivery system. Unilamellar vesicles were spontaneously formed by dissolving AOT-BHD in water and their toxicity was evaluated through in vitro and in vivo assays. Cell membrane permeability assays (hemolytic activity, Trypan blue assay) and cellular survival or proliferation (MTT assay) were performed. The results showed that only the highest concentration of vesicles tested (2 mg mL-1) diminished the red blood cells' resistance. In vivo toxicity evaluation was carried out on mice through lethal dose 50 (LD50) experiments. The safety for living organisms in doses lower than 0.05 mg mL-1 and the acid pH stability makes our AOT-BHD vesicles a very promising candidate for oral drug delivery.Fil: Stagnoli, Antonela Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Luna, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Villa, Cristian C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Niebylski, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; ArgentinaFil: Moyano, Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Correa, Nestor Mariano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaFil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaRoyal Society of Chemistry2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61519Stagnoli, Antonela Soledad; Luna, Maria Alejandra; Villa, Cristian C.; Alustiza, Fabrisio Eduardo; Niebylski, Ana Maria; et al.; Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation; Royal Society of Chemistry; RSC Advances; 7; 9; 12-2016; 5372-53802046-2069CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C6RA27020D#!divAbstractinfo:eu-repo/semantics/altIdentifier/doi/10.1039/c6ra27020dinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:40Zoai:ri.conicet.gov.ar:11336/61519instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:40.973CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
title Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
spellingShingle Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
Stagnoli, Antonela Soledad
CATANIONIC
AOT-BHD
VESICLES
title_short Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
title_full Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
title_fullStr Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
title_full_unstemmed Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
title_sort Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation
dc.creator.none.fl_str_mv Stagnoli, Antonela Soledad
Luna, Maria Alejandra
Villa, Cristian C.
Alustiza, Fabrisio Eduardo
Niebylski, Ana Maria
Moyano, Fernando
Correa, Nestor Mariano
Falcone, Ruben Dario
author Stagnoli, Antonela Soledad
author_facet Stagnoli, Antonela Soledad
Luna, Maria Alejandra
Villa, Cristian C.
Alustiza, Fabrisio Eduardo
Niebylski, Ana Maria
Moyano, Fernando
Correa, Nestor Mariano
Falcone, Ruben Dario
author_role author
author2 Luna, Maria Alejandra
Villa, Cristian C.
Alustiza, Fabrisio Eduardo
Niebylski, Ana Maria
Moyano, Fernando
Correa, Nestor Mariano
Falcone, Ruben Dario
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CATANIONIC
AOT-BHD
VESICLES
topic CATANIONIC
AOT-BHD
VESICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We evaluate in vitro and in vivo toxicity and stability in an acidic environment of new vesicles formed by the catanionic surfactant bis(2-ethylhexyl) sulfosuccinate benzyl-n-hexadecyldimethylammonium (AOT-BHD) in order to investigate their potential application as an oral drug delivery system. Unilamellar vesicles were spontaneously formed by dissolving AOT-BHD in water and their toxicity was evaluated through in vitro and in vivo assays. Cell membrane permeability assays (hemolytic activity, Trypan blue assay) and cellular survival or proliferation (MTT assay) were performed. The results showed that only the highest concentration of vesicles tested (2 mg mL-1) diminished the red blood cells' resistance. In vivo toxicity evaluation was carried out on mice through lethal dose 50 (LD50) experiments. The safety for living organisms in doses lower than 0.05 mg mL-1 and the acid pH stability makes our AOT-BHD vesicles a very promising candidate for oral drug delivery.
Fil: Stagnoli, Antonela Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Luna, Maria Alejandra. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Villa, Cristian C.. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Alustiza, Fabrisio Eduardo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Niebylski, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Biología Molecular; Argentina
Fil: Moyano, Fernando. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Correa, Nestor Mariano. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Fil: Falcone, Ruben Dario. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description We evaluate in vitro and in vivo toxicity and stability in an acidic environment of new vesicles formed by the catanionic surfactant bis(2-ethylhexyl) sulfosuccinate benzyl-n-hexadecyldimethylammonium (AOT-BHD) in order to investigate their potential application as an oral drug delivery system. Unilamellar vesicles were spontaneously formed by dissolving AOT-BHD in water and their toxicity was evaluated through in vitro and in vivo assays. Cell membrane permeability assays (hemolytic activity, Trypan blue assay) and cellular survival or proliferation (MTT assay) were performed. The results showed that only the highest concentration of vesicles tested (2 mg mL-1) diminished the red blood cells' resistance. In vivo toxicity evaluation was carried out on mice through lethal dose 50 (LD50) experiments. The safety for living organisms in doses lower than 0.05 mg mL-1 and the acid pH stability makes our AOT-BHD vesicles a very promising candidate for oral drug delivery.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61519
Stagnoli, Antonela Soledad; Luna, Maria Alejandra; Villa, Cristian C.; Alustiza, Fabrisio Eduardo; Niebylski, Ana Maria; et al.; Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation; Royal Society of Chemistry; RSC Advances; 7; 9; 12-2016; 5372-5380
2046-2069
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61519
identifier_str_mv Stagnoli, Antonela Soledad; Luna, Maria Alejandra; Villa, Cristian C.; Alustiza, Fabrisio Eduardo; Niebylski, Ana Maria; et al.; Unique catanionic vesicles as a potential "nano-Taxi" for drug delivery systems. In vitro and in vivo biocompatibility evaluation; Royal Society of Chemistry; RSC Advances; 7; 9; 12-2016; 5372-5380
2046-2069
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C6RA27020D#!divAbstract
info:eu-repo/semantics/altIdentifier/doi/10.1039/c6ra27020d
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269476670668800
score 13.13397

Publicaciones similares