CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization

Autores
Ernesto, Juan Ignacio; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Vasen, Gustavo; Martínez López, Pablo; Orta, Gerardo; Figueiras Fierro, Dulce; de la Vega Beltrán, José L.; Moreno, Ignacio A.; Guidobaldi, Héctor Alejandro; Giojalas, Laura Cecilia; Darszon, Alberto; Cohen, Debora Juana; Cuasnicu, Patricia Sara
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Calcium-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca2+ channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1-knockout females is impaired due to a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch-clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca2+ channel involved in hyperactivation and essential for fertility. Given the critical role of Ca2+ forsperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulatesperm hyperactivation and orientation for successful penetration of the cumulus during fertilization.
Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Martínez López, Pablo. Universidad Nacional Autónoma de México; México
Fil: Orta, Gerardo . Universidad Nacional Autónoma de México; México
Fil: Figueiras Fierro, Dulce. Universidad Nacional Autónoma de México; México
Fil: de la Vega Beltrán, José L. . Universidad Nacional Autónoma de México; México
Fil: Moreno, Ignacio A. . Fertility Patagonia; Argentina
Fil: Guidobaldi, Héctor Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Giojalas, Laura Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Darszon, Alberto . Universidad Nacional Autónoma de México; México
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
CRISP
QUIMIOTAXIS
HIPERACTIVACION
CATSPER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10347
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10347
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilizationErnesto, Juan IgnacioWeigel Muñoz, MarianaBattistone, Maria AgustinaVasen, GustavoMartínez López, PabloOrta, Gerardo Figueiras Fierro, Dulcede la Vega Beltrán, José L. Moreno, Ignacio A. Guidobaldi, Héctor AlejandroGiojalas, Laura CeciliaDarszon, Alberto Cohen, Debora JuanaCuasnicu, Patricia SaraCRISPQUIMIOTAXISHIPERACTIVACIONCATSPERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Calcium-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca2+ channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1-knockout females is impaired due to a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch-clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca2+ channel involved in hyperactivation and essential for fertility. Given the critical role of Ca2+ forsperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulatesperm hyperactivation and orientation for successful penetration of the cumulus during fertilization.Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Martínez López, Pablo. Universidad Nacional Autónoma de México; MéxicoFil: Orta, Gerardo . Universidad Nacional Autónoma de México; MéxicoFil: Figueiras Fierro, Dulce. Universidad Nacional Autónoma de México; MéxicoFil: de la Vega Beltrán, José L. . Universidad Nacional Autónoma de México; MéxicoFil: Moreno, Ignacio A. . Fertility Patagonia; ArgentinaFil: Guidobaldi, Héctor Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Giojalas, Laura Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Darszon, Alberto . Universidad Nacional Autónoma de México; MéxicoFil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaRockefeller Univ Press2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10347Ernesto, Juan Ignacio; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Vasen, Gustavo; Martínez López, Pablo; et al.; CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization; Rockefeller Univ Press; Journal Of Cell Biology; 210; 7; 7-2015; 1213-12240021-95251540-8140enginfo:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/210/7/1213.shortinfo:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201412041info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26416967/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:45Zoai:ri.conicet.gov.ar:11336/10347instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:46.068CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
spellingShingle CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
Ernesto, Juan Ignacio
CRISP
QUIMIOTAXIS
HIPERACTIVACION
CATSPER
title_short CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_full CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_fullStr CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_full_unstemmed CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
title_sort CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization
dc.creator.none.fl_str_mv Ernesto, Juan Ignacio
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Vasen, Gustavo
Martínez López, Pablo
Orta, Gerardo
Figueiras Fierro, Dulce
de la Vega Beltrán, José L.
Moreno, Ignacio A.
Guidobaldi, Héctor Alejandro
Giojalas, Laura Cecilia
Darszon, Alberto
Cohen, Debora Juana
Cuasnicu, Patricia Sara
author Ernesto, Juan Ignacio
author_facet Ernesto, Juan Ignacio
Weigel Muñoz, Mariana
Battistone, Maria Agustina
Vasen, Gustavo
Martínez López, Pablo
Orta, Gerardo
Figueiras Fierro, Dulce
de la Vega Beltrán, José L.
Moreno, Ignacio A.
Guidobaldi, Héctor Alejandro
Giojalas, Laura Cecilia
Darszon, Alberto
Cohen, Debora Juana
Cuasnicu, Patricia Sara
author_role author
author2 Weigel Muñoz, Mariana
Battistone, Maria Agustina
Vasen, Gustavo
Martínez López, Pablo
Orta, Gerardo
Figueiras Fierro, Dulce
de la Vega Beltrán, José L.
Moreno, Ignacio A.
Guidobaldi, Héctor Alejandro
Giojalas, Laura Cecilia
Darszon, Alberto
Cohen, Debora Juana
Cuasnicu, Patricia Sara
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CRISP
QUIMIOTAXIS
HIPERACTIVACION
CATSPER
topic CRISP
QUIMIOTAXIS
HIPERACTIVACION
CATSPER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Calcium-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca2+ channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1-knockout females is impaired due to a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch-clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca2+ channel involved in hyperactivation and essential for fertility. Given the critical role of Ca2+ forsperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulatesperm hyperactivation and orientation for successful penetration of the cumulus during fertilization.
Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Battistone, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Martínez López, Pablo. Universidad Nacional Autónoma de México; México
Fil: Orta, Gerardo . Universidad Nacional Autónoma de México; México
Fil: Figueiras Fierro, Dulce. Universidad Nacional Autónoma de México; México
Fil: de la Vega Beltrán, José L. . Universidad Nacional Autónoma de México; México
Fil: Moreno, Ignacio A. . Fertility Patagonia; Argentina
Fil: Guidobaldi, Héctor Alejandro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Giojalas, Laura Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Biología Celular y Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Darszon, Alberto . Universidad Nacional Autónoma de México; México
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Calcium-dependent mechanisms are critical for successful completion of fertilization. Here, we demonstrate that CRISP1, a sperm protein involved in mammalian fertilization, is also present in the female gamete and capable of modulating key sperm Ca2+ channels. Specifically, we show that CRISP1 is expressed by the cumulus cells that surround the egg and that fertilization of cumulus-oocyte complexes from CRISP1-knockout females is impaired due to a failure of sperm to penetrate the cumulus. We provide evidence that CRISP1 stimulates sperm orientation by modulating sperm hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, patch-clamping of sperm revealed that CRISP1 has the ability to regulate CatSper, the principal sperm Ca2+ channel involved in hyperactivation and essential for fertility. Given the critical role of Ca2+ forsperm motility, we propose a novel CRISP1-mediated fine-tuning mechanism to regulatesperm hyperactivation and orientation for successful penetration of the cumulus during fertilization.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10347
Ernesto, Juan Ignacio; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Vasen, Gustavo; Martínez López, Pablo; et al.; CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization; Rockefeller Univ Press; Journal Of Cell Biology; 210; 7; 7-2015; 1213-1224
0021-9525
1540-8140
url http://hdl.handle.net/11336/10347
identifier_str_mv Ernesto, Juan Ignacio; Weigel Muñoz, Mariana; Battistone, Maria Agustina; Vasen, Gustavo; Martínez López, Pablo; et al.; CRISP1 as a novel CatSper regulator that modulates sperm motility and orientation during fertilization; Rockefeller Univ Press; Journal Of Cell Biology; 210; 7; 7-2015; 1213-1224
0021-9525
1540-8140
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/210/7/1213.short
info:eu-repo/semantics/altIdentifier/doi/10.1083/jcb.201412041
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26416967/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Rockefeller Univ Press
publisher.none.fl_str_mv Rockefeller Univ Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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