Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity

Autores
Ale, Cesar Emmanuel; Torres Luque, Andrea; Gonzalez Moreno, Candelaria; Otero, María Claudia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Probiotics containing autochthonous Lactic Acid Bacteria (LAB) as local treatment in the Bovine Reproductive Tract (BRT)was proposed as a sustainable alternative to prevent outcome of pathogens colonization in the postpartum uterus in cows.Microencapsulation of these LABs could improve their survival during stressing conditions and promote the intimate contact betweenthe veterinarian form and the vaginal mucosa. In this work, emulsion-ionic gelation technique was applied to encapsulate bovine LABstrains in an alginate (3%) matrix. Optical and scanning electron microscopic evaluations showed spheroidal particles (12-48 µm)with a fully charge of LAB; the average load ranged 8.98 ± 0.15 to 8.06 ± 0.21 log CFU/g. The microencapsulated lactic acid bacteria(LAB-MCs) stability was evaluated during lyophilization [in Skim Milk (SM), or Neutral Distilled Water (NDW)] and storage (at 4°Cup to 90 days). SM represented a significant high protection to the lyophilization. Also, the alginate microencapsulation improvedthe LAB strain resistance when freeze-dried in water, comparing to known sensibility of LAB free cells. L. gasseri CRL 1412 showedsimilar resistance in both, NDW (0.70 ± 0.05) and SM (0.72 ± 0.05); and their microcapsules (MCs) exhibited antagonistic activityagainst E. coli 99/14 (pathogen from Bovine metritis) when cultured together; contrary, in co-culture with empty-MCs no inhibitionwas observed. To evaluate the microencapsulation process, different parameters were estimated: Encapsulation Factor (EF) (rangedbetween 0.76 ± 0.03 and 0.85 ± 0.08) and Encapsulating Efficiency (EE) (average EE%=75%) none significant differences (LSDFishertest, P<0.05) were observed between LAB strains. Taking account the weight of the materials, the calculated average yieldwas 50.5%. The standardized encapsulation conditions allowed selected L. gasseri CRL 1412-MCs as potential systems to beincluded in formulations to restore vaginal microbiota to prevent metritis in cows
Fil: Ale, Cesar Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Torres Luque, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Materia
BOVINE LACTOBACILLUS
EMULSION-GELATION
MICROENCAPSULATION
E. COLI
METRITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/50477
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/50477
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activityAle, Cesar EmmanuelTorres Luque, AndreaGonzalez Moreno, CandelariaOtero, María ClaudiaBOVINE LACTOBACILLUSEMULSION-GELATIONMICROENCAPSULATIONE. COLIMETRITIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Probiotics containing autochthonous Lactic Acid Bacteria (LAB) as local treatment in the Bovine Reproductive Tract (BRT)was proposed as a sustainable alternative to prevent outcome of pathogens colonization in the postpartum uterus in cows.Microencapsulation of these LABs could improve their survival during stressing conditions and promote the intimate contact betweenthe veterinarian form and the vaginal mucosa. In this work, emulsion-ionic gelation technique was applied to encapsulate bovine LABstrains in an alginate (3%) matrix. Optical and scanning electron microscopic evaluations showed spheroidal particles (12-48 µm)with a fully charge of LAB; the average load ranged 8.98 ± 0.15 to 8.06 ± 0.21 log CFU/g. The microencapsulated lactic acid bacteria(LAB-MCs) stability was evaluated during lyophilization [in Skim Milk (SM), or Neutral Distilled Water (NDW)] and storage (at 4°Cup to 90 days). SM represented a significant high protection to the lyophilization. Also, the alginate microencapsulation improvedthe LAB strain resistance when freeze-dried in water, comparing to known sensibility of LAB free cells. L. gasseri CRL 1412 showedsimilar resistance in both, NDW (0.70 ± 0.05) and SM (0.72 ± 0.05); and their microcapsules (MCs) exhibited antagonistic activityagainst E. coli 99/14 (pathogen from Bovine metritis) when cultured together; contrary, in co-culture with empty-MCs no inhibitionwas observed. To evaluate the microencapsulation process, different parameters were estimated: Encapsulation Factor (EF) (rangedbetween 0.76 ± 0.03 and 0.85 ± 0.08) and Encapsulating Efficiency (EE) (average EE%=75%) none significant differences (LSDFishertest, P<0.05) were observed between LAB strains. Taking account the weight of the materials, the calculated average yieldwas 50.5%. The standardized encapsulation conditions allowed selected L. gasseri CRL 1412-MCs as potential systems to beincluded in formulations to restore vaginal microbiota to prevent metritis in cowsFil: Ale, Cesar Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Torres Luque, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaOMICS international2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/50477Ale, Cesar Emmanuel; Torres Luque, Andrea; Gonzalez Moreno, Candelaria; Otero, María Claudia; Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity; OMICS international; Journal of Bioprocessing & Biotechniques; 5; 11; 11-20152155-9821CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.omicsonline.org/open-access/microencapsulation-of-bovine-vaginal-lactobacilli-in-alginate-usingemulsiongelation-freezedrying-storage-and-antimicrobial-activity-2155-9821-1000262.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9821.1000262info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:38:01Zoai:ri.conicet.gov.ar:11336/50477instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:38:02.089CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
title Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
spellingShingle Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
Ale, Cesar Emmanuel
BOVINE LACTOBACILLUS
EMULSION-GELATION
MICROENCAPSULATION
E. COLI
METRITIS
title_short Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
title_full Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
title_fullStr Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
title_full_unstemmed Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
title_sort Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity
dc.creator.none.fl_str_mv Ale, Cesar Emmanuel
Torres Luque, Andrea
Gonzalez Moreno, Candelaria
Otero, María Claudia
author Ale, Cesar Emmanuel
author_facet Ale, Cesar Emmanuel
Torres Luque, Andrea
Gonzalez Moreno, Candelaria
Otero, María Claudia
author_role author
author2 Torres Luque, Andrea
Gonzalez Moreno, Candelaria
Otero, María Claudia
author2_role author
author
author
dc.subject.none.fl_str_mv BOVINE LACTOBACILLUS
EMULSION-GELATION
MICROENCAPSULATION
E. COLI
METRITIS
topic BOVINE LACTOBACILLUS
EMULSION-GELATION
MICROENCAPSULATION
E. COLI
METRITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Probiotics containing autochthonous Lactic Acid Bacteria (LAB) as local treatment in the Bovine Reproductive Tract (BRT)was proposed as a sustainable alternative to prevent outcome of pathogens colonization in the postpartum uterus in cows.Microencapsulation of these LABs could improve their survival during stressing conditions and promote the intimate contact betweenthe veterinarian form and the vaginal mucosa. In this work, emulsion-ionic gelation technique was applied to encapsulate bovine LABstrains in an alginate (3%) matrix. Optical and scanning electron microscopic evaluations showed spheroidal particles (12-48 µm)with a fully charge of LAB; the average load ranged 8.98 ± 0.15 to 8.06 ± 0.21 log CFU/g. The microencapsulated lactic acid bacteria(LAB-MCs) stability was evaluated during lyophilization [in Skim Milk (SM), or Neutral Distilled Water (NDW)] and storage (at 4°Cup to 90 days). SM represented a significant high protection to the lyophilization. Also, the alginate microencapsulation improvedthe LAB strain resistance when freeze-dried in water, comparing to known sensibility of LAB free cells. L. gasseri CRL 1412 showedsimilar resistance in both, NDW (0.70 ± 0.05) and SM (0.72 ± 0.05); and their microcapsules (MCs) exhibited antagonistic activityagainst E. coli 99/14 (pathogen from Bovine metritis) when cultured together; contrary, in co-culture with empty-MCs no inhibitionwas observed. To evaluate the microencapsulation process, different parameters were estimated: Encapsulation Factor (EF) (rangedbetween 0.76 ± 0.03 and 0.85 ± 0.08) and Encapsulating Efficiency (EE) (average EE%=75%) none significant differences (LSDFishertest, P<0.05) were observed between LAB strains. Taking account the weight of the materials, the calculated average yieldwas 50.5%. The standardized encapsulation conditions allowed selected L. gasseri CRL 1412-MCs as potential systems to beincluded in formulations to restore vaginal microbiota to prevent metritis in cows
Fil: Ale, Cesar Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Torres Luque, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description Probiotics containing autochthonous Lactic Acid Bacteria (LAB) as local treatment in the Bovine Reproductive Tract (BRT)was proposed as a sustainable alternative to prevent outcome of pathogens colonization in the postpartum uterus in cows.Microencapsulation of these LABs could improve their survival during stressing conditions and promote the intimate contact betweenthe veterinarian form and the vaginal mucosa. In this work, emulsion-ionic gelation technique was applied to encapsulate bovine LABstrains in an alginate (3%) matrix. Optical and scanning electron microscopic evaluations showed spheroidal particles (12-48 µm)with a fully charge of LAB; the average load ranged 8.98 ± 0.15 to 8.06 ± 0.21 log CFU/g. The microencapsulated lactic acid bacteria(LAB-MCs) stability was evaluated during lyophilization [in Skim Milk (SM), or Neutral Distilled Water (NDW)] and storage (at 4°Cup to 90 days). SM represented a significant high protection to the lyophilization. Also, the alginate microencapsulation improvedthe LAB strain resistance when freeze-dried in water, comparing to known sensibility of LAB free cells. L. gasseri CRL 1412 showedsimilar resistance in both, NDW (0.70 ± 0.05) and SM (0.72 ± 0.05); and their microcapsules (MCs) exhibited antagonistic activityagainst E. coli 99/14 (pathogen from Bovine metritis) when cultured together; contrary, in co-culture with empty-MCs no inhibitionwas observed. To evaluate the microencapsulation process, different parameters were estimated: Encapsulation Factor (EF) (rangedbetween 0.76 ± 0.03 and 0.85 ± 0.08) and Encapsulating Efficiency (EE) (average EE%=75%) none significant differences (LSDFishertest, P<0.05) were observed between LAB strains. Taking account the weight of the materials, the calculated average yieldwas 50.5%. The standardized encapsulation conditions allowed selected L. gasseri CRL 1412-MCs as potential systems to beincluded in formulations to restore vaginal microbiota to prevent metritis in cows
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/50477
Ale, Cesar Emmanuel; Torres Luque, Andrea; Gonzalez Moreno, Candelaria; Otero, María Claudia; Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity; OMICS international; Journal of Bioprocessing & Biotechniques; 5; 11; 11-2015
2155-9821
CONICET Digital
CONICET
url http://hdl.handle.net/11336/50477
identifier_str_mv Ale, Cesar Emmanuel; Torres Luque, Andrea; Gonzalez Moreno, Candelaria; Otero, María Claudia; Microencapsulation of bovine vaginal lactobacilli in alginate using emulsion-gelation: freeze-drying, storage and antimicrobial activity; OMICS international; Journal of Bioprocessing & Biotechniques; 5; 11; 11-2015
2155-9821
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.omicsonline.org/open-access/microencapsulation-of-bovine-vaginal-lactobacilli-in-alginate-usingemulsiongelation-freezedrying-storage-and-antimicrobial-activity-2155-9821-1000262.pdf
info:eu-repo/semantics/altIdentifier/doi/10.4172/2155-9821.1000262
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS international
publisher.none.fl_str_mv OMICS international
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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