Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C

Autores
Garcia Castillo, Valeria; Komatsu, Ryoya; Clua, Maria Patricia; Indo, Yuhki; Takagi, Michihiro; Salva, Maria Susana; Islam, M. Aminul; Alvarez, Gladis Susana; Takahashi, Hideki; Garcia Cancino, Apolinaria; Kitazawa, Haruki; Villena, Julio Cesar
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacillus fermentum UCO-979C, a strain isolated from a human stomach, was previously characterized by its potential probiotic properties. The UCO-979C strain displayed the ability to beneficially regulate the innate immune response triggered by Helicobacter pylori infection in human gastric epithelial cells. In this work, we conducted further in vitro studies in intestinal epithelial cells (IECs) and in vivo experiments in mice in order to characterize the potential immunomodulatory effects of L. fermentum UCO-979C on the intestinal mucosa. Results demonstrated that the UCO-979C strain is capable to differentially modulate the immune response of IECs triggered by Toll-like receptor 4 (TLR4) activation through the modulation of TLR negative regulators' expression. In addition, we demonstrated for the first time that L. fermentum UCO-979C is able to exert its immunomodulatory effect in the intestinal mucosa in vivo. The feeding of mice with L. fermentum UCO-979C significantly increased the production of intestinal IFN-γ, stimulated intestinal and peritoneal macrophages and increased the number of Peyer's patches CD4+ T cells. In addition, L. fermentum UCO-979C augmented intestinal IL-6, reduced the number of immature B220+CD24high B cells from Peyer's patches, enhanced the number of mature B B220+CD24low cells, and significantly increased intestinal IgA content. The results of this work revealed that L. fermentum UCO-979C has several characteristics making it an excellent candidate for the development of immunobiotic functional foods aimed to differentially regulate immune responses against gastric and intestinal pathogens.
Fil: Garcia Castillo, Valeria. Tohoku University; Japón. Universidad de Concepción; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Komatsu, Ryoya. Tohoku University; Japón
Fil: Clua, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Indo, Yuhki. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Islam, M. Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón
Fil: Alvarez, Gladis Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Garcia Cancino, Apolinaria. Universidad de Concepción; Chile
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Materia
IMMUNOBIOTICS
INTESTINAL IMMUNITY
LACTOBACILLUS FERMENTUM UCO-979C
MACROPHAGES
PIE CELLS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96624
Acceder
id CONICETDig_8d312db71560c2e873d01339326af160
oai_identifier_str oai:ri.conicet.gov.ar:11336/96624
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979CGarcia Castillo, ValeriaKomatsu, RyoyaClua, Maria PatriciaIndo, YuhkiTakagi, MichihiroSalva, Maria SusanaIslam, M. AminulAlvarez, Gladis SusanaTakahashi, HidekiGarcia Cancino, ApolinariaKitazawa, HarukiVillena, Julio CesarIMMUNOBIOTICSINTESTINAL IMMUNITYLACTOBACILLUS FERMENTUM UCO-979CMACROPHAGESPIE CELLShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactobacillus fermentum UCO-979C, a strain isolated from a human stomach, was previously characterized by its potential probiotic properties. The UCO-979C strain displayed the ability to beneficially regulate the innate immune response triggered by Helicobacter pylori infection in human gastric epithelial cells. In this work, we conducted further in vitro studies in intestinal epithelial cells (IECs) and in vivo experiments in mice in order to characterize the potential immunomodulatory effects of L. fermentum UCO-979C on the intestinal mucosa. Results demonstrated that the UCO-979C strain is capable to differentially modulate the immune response of IECs triggered by Toll-like receptor 4 (TLR4) activation through the modulation of TLR negative regulators' expression. In addition, we demonstrated for the first time that L. fermentum UCO-979C is able to exert its immunomodulatory effect in the intestinal mucosa in vivo. The feeding of mice with L. fermentum UCO-979C significantly increased the production of intestinal IFN-γ, stimulated intestinal and peritoneal macrophages and increased the number of Peyer's patches CD4+ T cells. In addition, L. fermentum UCO-979C augmented intestinal IL-6, reduced the number of immature B220+CD24high B cells from Peyer's patches, enhanced the number of mature B B220+CD24low cells, and significantly increased intestinal IgA content. The results of this work revealed that L. fermentum UCO-979C has several characteristics making it an excellent candidate for the development of immunobiotic functional foods aimed to differentially regulate immune responses against gastric and intestinal pathogens.Fil: Garcia Castillo, Valeria. Tohoku University; Japón. Universidad de Concepción; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Komatsu, Ryoya. Tohoku University; JapónFil: Clua, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Indo, Yuhki. Tohoku University; JapónFil: Takagi, Michihiro. Tohoku University; JapónFil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Islam, M. Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; JapónFil: Alvarez, Gladis Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Takahashi, Hideki. Tohoku University; JapónFil: Garcia Cancino, Apolinaria. Universidad de Concepción; ChileFil: Kitazawa, Haruki. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFrontiers Media S.A.2019-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96624Garcia Castillo, Valeria; Komatsu, Ryoya; Clua, Maria Patricia; Indo, Yuhki; Takagi, Michihiro; et al.; Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C; Frontiers Media S.A.; Frontiers in Immunology; 10; 13-6-2019; 1-141664-32241664-3224CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2019.01376info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2019.01376/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:13:46Zoai:ri.conicet.gov.ar:11336/96624instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:13:46.92CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
title Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
spellingShingle Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
Garcia Castillo, Valeria
IMMUNOBIOTICS
INTESTINAL IMMUNITY
LACTOBACILLUS FERMENTUM UCO-979C
MACROPHAGES
PIE CELLS
title_short Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
title_full Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
title_fullStr Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
title_full_unstemmed Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
title_sort Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C
dc.creator.none.fl_str_mv Garcia Castillo, Valeria
Komatsu, Ryoya
Clua, Maria Patricia
Indo, Yuhki
Takagi, Michihiro
Salva, Maria Susana
Islam, M. Aminul
Alvarez, Gladis Susana
Takahashi, Hideki
Garcia Cancino, Apolinaria
Kitazawa, Haruki
Villena, Julio Cesar
author Garcia Castillo, Valeria
author_facet Garcia Castillo, Valeria
Komatsu, Ryoya
Clua, Maria Patricia
Indo, Yuhki
Takagi, Michihiro
Salva, Maria Susana
Islam, M. Aminul
Alvarez, Gladis Susana
Takahashi, Hideki
Garcia Cancino, Apolinaria
Kitazawa, Haruki
Villena, Julio Cesar
author_role author
author2 Komatsu, Ryoya
Clua, Maria Patricia
Indo, Yuhki
Takagi, Michihiro
Salva, Maria Susana
Islam, M. Aminul
Alvarez, Gladis Susana
Takahashi, Hideki
Garcia Cancino, Apolinaria
Kitazawa, Haruki
Villena, Julio Cesar
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv IMMUNOBIOTICS
INTESTINAL IMMUNITY
LACTOBACILLUS FERMENTUM UCO-979C
MACROPHAGES
PIE CELLS
topic IMMUNOBIOTICS
INTESTINAL IMMUNITY
LACTOBACILLUS FERMENTUM UCO-979C
MACROPHAGES
PIE CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactobacillus fermentum UCO-979C, a strain isolated from a human stomach, was previously characterized by its potential probiotic properties. The UCO-979C strain displayed the ability to beneficially regulate the innate immune response triggered by Helicobacter pylori infection in human gastric epithelial cells. In this work, we conducted further in vitro studies in intestinal epithelial cells (IECs) and in vivo experiments in mice in order to characterize the potential immunomodulatory effects of L. fermentum UCO-979C on the intestinal mucosa. Results demonstrated that the UCO-979C strain is capable to differentially modulate the immune response of IECs triggered by Toll-like receptor 4 (TLR4) activation through the modulation of TLR negative regulators' expression. In addition, we demonstrated for the first time that L. fermentum UCO-979C is able to exert its immunomodulatory effect in the intestinal mucosa in vivo. The feeding of mice with L. fermentum UCO-979C significantly increased the production of intestinal IFN-γ, stimulated intestinal and peritoneal macrophages and increased the number of Peyer's patches CD4+ T cells. In addition, L. fermentum UCO-979C augmented intestinal IL-6, reduced the number of immature B220+CD24high B cells from Peyer's patches, enhanced the number of mature B B220+CD24low cells, and significantly increased intestinal IgA content. The results of this work revealed that L. fermentum UCO-979C has several characteristics making it an excellent candidate for the development of immunobiotic functional foods aimed to differentially regulate immune responses against gastric and intestinal pathogens.
Fil: Garcia Castillo, Valeria. Tohoku University; Japón. Universidad de Concepción; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Komatsu, Ryoya. Tohoku University; Japón
Fil: Clua, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Indo, Yuhki. Tohoku University; Japón
Fil: Takagi, Michihiro. Tohoku University; Japón
Fil: Salva, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Islam, M. Aminul. Bangladesh Agricultural University; Bangladesh. Tohoku University; Japón
Fil: Alvarez, Gladis Susana. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Takahashi, Hideki. Tohoku University; Japón
Fil: Garcia Cancino, Apolinaria. Universidad de Concepción; Chile
Fil: Kitazawa, Haruki. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
description Lactobacillus fermentum UCO-979C, a strain isolated from a human stomach, was previously characterized by its potential probiotic properties. The UCO-979C strain displayed the ability to beneficially regulate the innate immune response triggered by Helicobacter pylori infection in human gastric epithelial cells. In this work, we conducted further in vitro studies in intestinal epithelial cells (IECs) and in vivo experiments in mice in order to characterize the potential immunomodulatory effects of L. fermentum UCO-979C on the intestinal mucosa. Results demonstrated that the UCO-979C strain is capable to differentially modulate the immune response of IECs triggered by Toll-like receptor 4 (TLR4) activation through the modulation of TLR negative regulators' expression. In addition, we demonstrated for the first time that L. fermentum UCO-979C is able to exert its immunomodulatory effect in the intestinal mucosa in vivo. The feeding of mice with L. fermentum UCO-979C significantly increased the production of intestinal IFN-γ, stimulated intestinal and peritoneal macrophages and increased the number of Peyer's patches CD4+ T cells. In addition, L. fermentum UCO-979C augmented intestinal IL-6, reduced the number of immature B220+CD24high B cells from Peyer's patches, enhanced the number of mature B B220+CD24low cells, and significantly increased intestinal IgA content. The results of this work revealed that L. fermentum UCO-979C has several characteristics making it an excellent candidate for the development of immunobiotic functional foods aimed to differentially regulate immune responses against gastric and intestinal pathogens.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96624
Garcia Castillo, Valeria; Komatsu, Ryoya; Clua, Maria Patricia; Indo, Yuhki; Takagi, Michihiro; et al.; Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C; Frontiers Media S.A.; Frontiers in Immunology; 10; 13-6-2019; 1-14
1664-3224
1664-3224
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96624
identifier_str_mv Garcia Castillo, Valeria; Komatsu, Ryoya; Clua, Maria Patricia; Indo, Yuhki; Takagi, Michihiro; et al.; Evaluation of the immunomodulatory activities of the probiotic strain lactobacillus fermentum UCO-979C; Frontiers Media S.A.; Frontiers in Immunology; 10; 13-6-2019; 1-14
1664-3224
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fimmu.2019.01376
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fimmu.2019.01376/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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