Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency

Autores
Ramírez, Leonor; Bartoli, Carlos Guillermo; Lamattina, Lorenzo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Iron is an essential micronutrient required for a wide variety of cellular functions in plant growth and development. Chlorosis is the first visible symptom in iron-deficient plants. Glutathione (GSH) and ascorbic acid (ASC) are multifunctional metabolites playing important roles in redox balancing. In this work, it was shown that GSH and ASC treatment prevented chlorosis and the accumulation of reactive oxygen species induced by iron deficiency in Arabidopsis leaves. In iron deficiency, GSH and ASC increased the activity of the heme protein ascorbate peroxidase at a similar level to that found in iron-sufficient seedlings. GSH was also able to preserve the levels of the iron–sulfur protein ferredoxin 2. GSH content decreased 25% in iron-deficient Arabidopsis seedlings, whereas the ASC levels were not affected. Taken together, these results showed that GSH and ASC supplementation protects Arabidopsis seedlings from iron deficiency, preserving cell redox homeostasis and improving internal iron availability.
Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bartoli, Carlos Guillermo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Arabidopsis Thaliana
Ascorbic Acid
Chlorosis
Glutathione
Iron Deficiency
Reactive Oxygen Species
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13128
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiencyRamírez, LeonorBartoli, Carlos GuillermoLamattina, LorenzoArabidopsis ThalianaAscorbic AcidChlorosisGlutathioneIron DeficiencyReactive Oxygen Specieshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Iron is an essential micronutrient required for a wide variety of cellular functions in plant growth and development. Chlorosis is the first visible symptom in iron-deficient plants. Glutathione (GSH) and ascorbic acid (ASC) are multifunctional metabolites playing important roles in redox balancing. In this work, it was shown that GSH and ASC treatment prevented chlorosis and the accumulation of reactive oxygen species induced by iron deficiency in Arabidopsis leaves. In iron deficiency, GSH and ASC increased the activity of the heme protein ascorbate peroxidase at a similar level to that found in iron-sufficient seedlings. GSH was also able to preserve the levels of the iron–sulfur protein ferredoxin 2. GSH content decreased 25% in iron-deficient Arabidopsis seedlings, whereas the ASC levels were not affected. Taken together, these results showed that GSH and ASC supplementation protects Arabidopsis seedlings from iron deficiency, preserving cell redox homeostasis and improving internal iron availability.Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bartoli, Carlos Guillermo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata; ArgentinaFil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; ArgentinaOxford University Press2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13128Ramírez, Leonor; Bartoli, Carlos Guillermo; Lamattina, Lorenzo; Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency; Oxford University Press; Journal of Experimental Botany; 64; 11; 6-2013; 3339-33490022-09571460-2431enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert172info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ert172info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:47:40Zoai:ri.conicet.gov.ar:11336/13128instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:47:40.949CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
title Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
spellingShingle Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
Ramírez, Leonor
Arabidopsis Thaliana
Ascorbic Acid
Chlorosis
Glutathione
Iron Deficiency
Reactive Oxygen Species
title_short Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
title_full Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
title_fullStr Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
title_full_unstemmed Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
title_sort Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency
dc.creator.none.fl_str_mv Ramírez, Leonor
Bartoli, Carlos Guillermo
Lamattina, Lorenzo
author Ramírez, Leonor
author_facet Ramírez, Leonor
Bartoli, Carlos Guillermo
Lamattina, Lorenzo
author_role author
author2 Bartoli, Carlos Guillermo
Lamattina, Lorenzo
author2_role author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Ascorbic Acid
Chlorosis
Glutathione
Iron Deficiency
Reactive Oxygen Species
topic Arabidopsis Thaliana
Ascorbic Acid
Chlorosis
Glutathione
Iron Deficiency
Reactive Oxygen Species
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Iron is an essential micronutrient required for a wide variety of cellular functions in plant growth and development. Chlorosis is the first visible symptom in iron-deficient plants. Glutathione (GSH) and ascorbic acid (ASC) are multifunctional metabolites playing important roles in redox balancing. In this work, it was shown that GSH and ASC treatment prevented chlorosis and the accumulation of reactive oxygen species induced by iron deficiency in Arabidopsis leaves. In iron deficiency, GSH and ASC increased the activity of the heme protein ascorbate peroxidase at a similar level to that found in iron-sufficient seedlings. GSH was also able to preserve the levels of the iron–sulfur protein ferredoxin 2. GSH content decreased 25% in iron-deficient Arabidopsis seedlings, whereas the ASC levels were not affected. Taken together, these results showed that GSH and ASC supplementation protects Arabidopsis seedlings from iron deficiency, preserving cell redox homeostasis and improving internal iron availability.
Fil: Ramírez, Leonor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bartoli, Carlos Guillermo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Mar del Plata; Argentina
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigaciones Biológicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
description Iron is an essential micronutrient required for a wide variety of cellular functions in plant growth and development. Chlorosis is the first visible symptom in iron-deficient plants. Glutathione (GSH) and ascorbic acid (ASC) are multifunctional metabolites playing important roles in redox balancing. In this work, it was shown that GSH and ASC treatment prevented chlorosis and the accumulation of reactive oxygen species induced by iron deficiency in Arabidopsis leaves. In iron deficiency, GSH and ASC increased the activity of the heme protein ascorbate peroxidase at a similar level to that found in iron-sufficient seedlings. GSH was also able to preserve the levels of the iron–sulfur protein ferredoxin 2. GSH content decreased 25% in iron-deficient Arabidopsis seedlings, whereas the ASC levels were not affected. Taken together, these results showed that GSH and ASC supplementation protects Arabidopsis seedlings from iron deficiency, preserving cell redox homeostasis and improving internal iron availability.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13128
Ramírez, Leonor; Bartoli, Carlos Guillermo; Lamattina, Lorenzo; Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency; Oxford University Press; Journal of Experimental Botany; 64; 11; 6-2013; 3339-3349
0022-0957
1460-2431
url http://hdl.handle.net/11336/13128
identifier_str_mv Ramírez, Leonor; Bartoli, Carlos Guillermo; Lamattina, Lorenzo; Glutathione and ascorbic acid protect Arabidopsis plants against detrimental effects of iron deficiency; Oxford University Press; Journal of Experimental Botany; 64; 11; 6-2013; 3339-3349
0022-0957
1460-2431
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/ert172
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ert172
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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