Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy

Autores
Wittstein, Kathrin; Cordsmeier, Arne; Lambert, Christopher; Wendt, L.; Sir, Esteban Benjamin; Weber, J.; Wurzler, N.; Petrini, Liliane E.; Stadler, Marc
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.
Fil: Wittstein, Kathrin. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Cordsmeier, Arne. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. Universitat Erlangen Nuremberg; Alemania
Fil: Lambert, Christopher. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Wendt, L.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Sir, Esteban Benjamin. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina
Fil: Weber, J.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Wurzler, N.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Petrini, Liliane E.. Via al Perato 15c; Suiza
Fil: Stadler, Marc. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Materia
DEMATOPHORA
GENUS RESURRECTION
ISOPIMARANE DITERPENOIDS
PF1022A
POLYTHETIC TAXONOMY
ROSELLINIA
XYLARIACEAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181889
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181889
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomyWittstein, KathrinCordsmeier, ArneLambert, ChristopherWendt, L.Sir, Esteban BenjaminWeber, J.Wurzler, N.Petrini, Liliane E.Stadler, MarcDEMATOPHORAGENUS RESURRECTIONISOPIMARANE DITERPENOIDSPF1022APOLYTHETIC TAXONOMYROSELLINIAXYLARIACEAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.Fil: Wittstein, Kathrin. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Cordsmeier, Arne. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. Universitat Erlangen Nuremberg; AlemaniaFil: Lambert, Christopher. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Wendt, L.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Sir, Esteban Benjamin. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Weber, J.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Wurzler, N.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaFil: Petrini, Liliane E.. Via al Perato 15c; SuizaFil: Stadler, Marc. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; AlemaniaCentraalbureau Schimmelculture2020-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181889Wittstein, Kathrin; Cordsmeier, Arne; Lambert, Christopher; Wendt, L.; Sir, Esteban Benjamin; et al.; Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy; Centraalbureau Schimmelculture; Studies In Mycology; 96; 6-2020; 1-160166-06161872-9797CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166061620300014info:eu-repo/semantics/altIdentifier/doi/10.1016/j.simyco.2020.01.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:04:28Zoai:ri.conicet.gov.ar:11336/181889instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:04:29.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
title Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
spellingShingle Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
Wittstein, Kathrin
DEMATOPHORA
GENUS RESURRECTION
ISOPIMARANE DITERPENOIDS
PF1022A
POLYTHETIC TAXONOMY
ROSELLINIA
XYLARIACEAE
title_short Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
title_full Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
title_fullStr Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
title_full_unstemmed Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
title_sort Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy
dc.creator.none.fl_str_mv Wittstein, Kathrin
Cordsmeier, Arne
Lambert, Christopher
Wendt, L.
Sir, Esteban Benjamin
Weber, J.
Wurzler, N.
Petrini, Liliane E.
Stadler, Marc
author Wittstein, Kathrin
author_facet Wittstein, Kathrin
Cordsmeier, Arne
Lambert, Christopher
Wendt, L.
Sir, Esteban Benjamin
Weber, J.
Wurzler, N.
Petrini, Liliane E.
Stadler, Marc
author_role author
author2 Cordsmeier, Arne
Lambert, Christopher
Wendt, L.
Sir, Esteban Benjamin
Weber, J.
Wurzler, N.
Petrini, Liliane E.
Stadler, Marc
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DEMATOPHORA
GENUS RESURRECTION
ISOPIMARANE DITERPENOIDS
PF1022A
POLYTHETIC TAXONOMY
ROSELLINIA
XYLARIACEAE
topic DEMATOPHORA
GENUS RESURRECTION
ISOPIMARANE DITERPENOIDS
PF1022A
POLYTHETIC TAXONOMY
ROSELLINIA
XYLARIACEAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.
Fil: Wittstein, Kathrin. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Cordsmeier, Arne. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. Universitat Erlangen Nuremberg; Alemania
Fil: Lambert, Christopher. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Wendt, L.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Sir, Esteban Benjamin. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina
Fil: Weber, J.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Wurzler, N.. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
Fil: Petrini, Liliane E.. Via al Perato 15c; Suiza
Fil: Stadler, Marc. Helmholtz-Zentrum für Infektionsforschung GmbH; Alemania. German Centre for Infection Research; Alemania
description Rosellinia (Xylariaceae) is a large, cosmopolitan genus comprising over 130 species that have been defined based mainly on the morphology of their sexual morphs. The genus comprises both lignicolous and saprotrophic species that are frequently isolated as endophytes from healthy host plants, and important plant pathogens. In order to evaluate the utility of molecular phylogeny and secondary metabolite profiling to achieve a better basis for their classification, a set of strains was selected for a multi-locus phylogeny inferred from a combination of the sequences of the internal transcribed spacer region (ITS), the large subunit (LSU) of the nuclear rDNA, beta-tubulin (TUB2) and the second largest subunit of the RNA polymerase II (RPB2). Concurrently, various strains were surveyed for production of secondary metabolites. Metabolite profiling relied on methods with high performance liquid chromatography with diode array and mass spectrometric detection (HPLC-DAD/MS) as well as preparative isolation of the major components after re-fermentation followed by structure elucidation using nuclear magnetic resonance (NMR) spectroscopy and high resolution mass spectrometry (HR-MS). Two new and nine known isopimarane diterpenoids were identified during our mycochemical studies of two selected Dematophora strains and the metabolites were tested for biological activity. In addition, the nematicidal cyclodepsipeptide PF1022 A was purified and identified from a culture of Rosellinia corticium, which is the first time that this endophyte-derived drug precursor has been identified unambiguously from an ascospore-derived isolate of a Rosellinia species. While the results of this first HPLC profiling were largely inconclusive regarding the utility of secondary metabolites as genus-specific chemotaxonomic markers, the phylogeny clearly showed that species featuring a dematophora-like asexual morph were included in a well-defined clade, for which the genus Dematophora is resurrected. Dematophora now comprises all previously known important plant pathogens in the genus such as D. arcuata, D. bunodes, D. necatrix and D. pepo, while Rosellinia s. str. comprises those species that are known to have a geniculosporium-like or nodulisporium-like asexual morph, or where the asexual morph remains unknown. The extensive morphological studies of L.E. Petrini served as a basis to transfer several further species from Rosellinia to Dematophora, based on the morphology of their asexual morphs. However, most species of Rosellinia and allies still need to be recollected in fresh state, cultured, and studied for their morphology and their phylogenetic affinities before the infrageneric relationships can be clarified.
publishDate 2020
dc.date.none.fl_str_mv 2020-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181889
Wittstein, Kathrin; Cordsmeier, Arne; Lambert, Christopher; Wendt, L.; Sir, Esteban Benjamin; et al.; Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy; Centraalbureau Schimmelculture; Studies In Mycology; 96; 6-2020; 1-16
0166-0616
1872-9797
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181889
identifier_str_mv Wittstein, Kathrin; Cordsmeier, Arne; Lambert, Christopher; Wendt, L.; Sir, Esteban Benjamin; et al.; Identification of Rosellinia species as producers of cyclodepsipeptide PF1022 A and resurrection of the genus Dematophora as inferred from polythetic taxonomy; Centraalbureau Schimmelculture; Studies In Mycology; 96; 6-2020; 1-16
0166-0616
1872-9797
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166061620300014
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.simyco.2020.01.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Centraalbureau Schimmelculture
publisher.none.fl_str_mv Centraalbureau Schimmelculture
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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