Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats

Autores
White, Verónica; Jawerbaum, Alicia Sandra; Mazzucco, María Belén; Gauster, Martin; Desoye, Gernot; Hiden, Ursula
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Diabetes in pregnancy affects fetal growth and development. The insulin/insulin-like growth factors (IGF) system comprising insulin, IGF, their receptors, and binding proteins, has been implicated in fetal growth regulation. This study tested the hypothesis that maternal diabetes alters the fetal insulin/IGF system in a tissue-specific manner. Methods: Wistar rats were rendered diabetic by neonatal administration of streptozotocin and mated with control rats. At day 21 of gestation, the weights of fetuses, placentas, and fetal organs (heart, lung, liver, stomach, intestine, and pancreas) were determined. Maternal and fetal plasma concentrations of insulin, IGF1, and IGF2 were measured by ELISA, and expression of IGF1, IGF2, IGF1R, IGF2R, IR, IGFBP1, BP2, and BP3 in placenta and fetal organs by qPCR. Results: The well-known increase in fetal growth in this model of mild diabetes is accompanied by elevated insulin and IGF1 levels and alterations of the insulin/IGF system in the fetus and the placenta. These alterations were organ and gene specific. The insulin/IGF system was generally upregulated, especially in the fetal heart, while it was downregulated in fetal lung. Conclusion: In our model of mild diabetes, the effect of maternal diabetes on fetal weight and fetal insulin/IGF system expression is organ specific with highly sensitive organs such as lung and heart, and organs that were less affected, such as stomach.
Fil: White, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Mazzucco, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Gauster, Martin. Medical University of Graz. Institute of Cell Biology, Histology and Embryology; Austria
Fil: Desoye, Gernot. Medical University of Graz. Department of Obstetrics and Gynaecology; Austria
Fil: Hiden, Ursula. Medical University of Graz. Department of Obstetrics and Gynaecology; Austria
Materia
Maternal Diabetes
Fetus
Placenta
Insulin-Igf
Pregnancy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13857
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13857
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in ratsWhite, VerónicaJawerbaum, Alicia SandraMazzucco, María BelénGauster, MartinDesoye, GernotHiden, UrsulaMaternal DiabetesFetusPlacentaInsulin-IgfPregnancyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Diabetes in pregnancy affects fetal growth and development. The insulin/insulin-like growth factors (IGF) system comprising insulin, IGF, their receptors, and binding proteins, has been implicated in fetal growth regulation. This study tested the hypothesis that maternal diabetes alters the fetal insulin/IGF system in a tissue-specific manner. Methods: Wistar rats were rendered diabetic by neonatal administration of streptozotocin and mated with control rats. At day 21 of gestation, the weights of fetuses, placentas, and fetal organs (heart, lung, liver, stomach, intestine, and pancreas) were determined. Maternal and fetal plasma concentrations of insulin, IGF1, and IGF2 were measured by ELISA, and expression of IGF1, IGF2, IGF1R, IGF2R, IR, IGFBP1, BP2, and BP3 in placenta and fetal organs by qPCR. Results: The well-known increase in fetal growth in this model of mild diabetes is accompanied by elevated insulin and IGF1 levels and alterations of the insulin/IGF system in the fetus and the placenta. These alterations were organ and gene specific. The insulin/IGF system was generally upregulated, especially in the fetal heart, while it was downregulated in fetal lung. Conclusion: In our model of mild diabetes, the effect of maternal diabetes on fetal weight and fetal insulin/IGF system expression is organ specific with highly sensitive organs such as lung and heart, and organs that were less affected, such as stomach.Fil: White, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Mazzucco, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Gauster, Martin. Medical University of Graz. Institute of Cell Biology, Histology and Embryology; AustriaFil: Desoye, Gernot. Medical University of Graz. Department of Obstetrics and Gynaecology; AustriaFil: Hiden, Ursula. Medical University of Graz. Department of Obstetrics and Gynaecology; AustriaNature Publishing Group2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13857White, Verónica; Jawerbaum, Alicia Sandra; Mazzucco, María Belén; Gauster, Martin; Desoye, Gernot; et al.; Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats; Nature Publishing Group; Pediatric Research; 77; 1-1; 1-2015; 48-550031-39981530-0447enginfo:eu-repo/semantics/altIdentifier/url/http://www.nature.com/pr/journal/v77/n1-1/full/pr2014139a.html#affil-authinfo:eu-repo/semantics/altIdentifier/doi/10.1038/pr.2014.139info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:53Zoai:ri.conicet.gov.ar:11336/13857instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:53.74CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
title Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
spellingShingle Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
White, Verónica
Maternal Diabetes
Fetus
Placenta
Insulin-Igf
Pregnancy
title_short Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
title_full Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
title_fullStr Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
title_full_unstemmed Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
title_sort Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats
dc.creator.none.fl_str_mv White, Verónica
Jawerbaum, Alicia Sandra
Mazzucco, María Belén
Gauster, Martin
Desoye, Gernot
Hiden, Ursula
author White, Verónica
author_facet White, Verónica
Jawerbaum, Alicia Sandra
Mazzucco, María Belén
Gauster, Martin
Desoye, Gernot
Hiden, Ursula
author_role author
author2 Jawerbaum, Alicia Sandra
Mazzucco, María Belén
Gauster, Martin
Desoye, Gernot
Hiden, Ursula
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Maternal Diabetes
Fetus
Placenta
Insulin-Igf
Pregnancy
topic Maternal Diabetes
Fetus
Placenta
Insulin-Igf
Pregnancy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Diabetes in pregnancy affects fetal growth and development. The insulin/insulin-like growth factors (IGF) system comprising insulin, IGF, their receptors, and binding proteins, has been implicated in fetal growth regulation. This study tested the hypothesis that maternal diabetes alters the fetal insulin/IGF system in a tissue-specific manner. Methods: Wistar rats were rendered diabetic by neonatal administration of streptozotocin and mated with control rats. At day 21 of gestation, the weights of fetuses, placentas, and fetal organs (heart, lung, liver, stomach, intestine, and pancreas) were determined. Maternal and fetal plasma concentrations of insulin, IGF1, and IGF2 were measured by ELISA, and expression of IGF1, IGF2, IGF1R, IGF2R, IR, IGFBP1, BP2, and BP3 in placenta and fetal organs by qPCR. Results: The well-known increase in fetal growth in this model of mild diabetes is accompanied by elevated insulin and IGF1 levels and alterations of the insulin/IGF system in the fetus and the placenta. These alterations were organ and gene specific. The insulin/IGF system was generally upregulated, especially in the fetal heart, while it was downregulated in fetal lung. Conclusion: In our model of mild diabetes, the effect of maternal diabetes on fetal weight and fetal insulin/IGF system expression is organ specific with highly sensitive organs such as lung and heart, and organs that were less affected, such as stomach.
Fil: White, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Jawerbaum, Alicia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Mazzucco, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Gauster, Martin. Medical University of Graz. Institute of Cell Biology, Histology and Embryology; Austria
Fil: Desoye, Gernot. Medical University of Graz. Department of Obstetrics and Gynaecology; Austria
Fil: Hiden, Ursula. Medical University of Graz. Department of Obstetrics and Gynaecology; Austria
description Background: Diabetes in pregnancy affects fetal growth and development. The insulin/insulin-like growth factors (IGF) system comprising insulin, IGF, their receptors, and binding proteins, has been implicated in fetal growth regulation. This study tested the hypothesis that maternal diabetes alters the fetal insulin/IGF system in a tissue-specific manner. Methods: Wistar rats were rendered diabetic by neonatal administration of streptozotocin and mated with control rats. At day 21 of gestation, the weights of fetuses, placentas, and fetal organs (heart, lung, liver, stomach, intestine, and pancreas) were determined. Maternal and fetal plasma concentrations of insulin, IGF1, and IGF2 were measured by ELISA, and expression of IGF1, IGF2, IGF1R, IGF2R, IR, IGFBP1, BP2, and BP3 in placenta and fetal organs by qPCR. Results: The well-known increase in fetal growth in this model of mild diabetes is accompanied by elevated insulin and IGF1 levels and alterations of the insulin/IGF system in the fetus and the placenta. These alterations were organ and gene specific. The insulin/IGF system was generally upregulated, especially in the fetal heart, while it was downregulated in fetal lung. Conclusion: In our model of mild diabetes, the effect of maternal diabetes on fetal weight and fetal insulin/IGF system expression is organ specific with highly sensitive organs such as lung and heart, and organs that were less affected, such as stomach.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13857
White, Verónica; Jawerbaum, Alicia Sandra; Mazzucco, María Belén; Gauster, Martin; Desoye, Gernot; et al.; Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats; Nature Publishing Group; Pediatric Research; 77; 1-1; 1-2015; 48-55
0031-3998
1530-0447
url http://hdl.handle.net/11336/13857
identifier_str_mv White, Verónica; Jawerbaum, Alicia Sandra; Mazzucco, María Belén; Gauster, Martin; Desoye, Gernot; et al.; Diabetes-associated changes in the fetal insulin/insulin-like growth factor system are organ specific in rats; Nature Publishing Group; Pediatric Research; 77; 1-1; 1-2015; 48-55
0031-3998
1530-0447
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/pr/journal/v77/n1-1/full/pr2014139a.html#affil-auth
info:eu-repo/semantics/altIdentifier/doi/10.1038/pr.2014.139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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