Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry

Autores
Errasti, María Eugenia; Caffini, Nestor Oscar; Lopez, Laura Maria Isabel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Most tanneries use high proportions of Na2S and CaO during the dehairing step, resulting in effluents of high alkalinity and large amounts of suspended solid, besides the risk of liberating the toxic H2S. Solid waste rich in protein is another environmental problem of tanneries. Enzymes are an interesting technological tool for industry due to their biodegradability, nontoxic nature, and nonpolluting effluent generation. In the leather industry, proteases have been chosen as a promising eco-friendly alternative to Na2S/CaO dehairing. Extracts with high proteolytic activity have been obtained from fruits of Bromeliaceae species: Bromelia balansae Mez (Bb), Bromelia hieronymi Mez (Bh), and Pseudananas macrodontes (Morr.) Harms (Pm). In this work, Bb, Bh, and Pm have been studied for application in the leather industry, focusing in their dehairing properties. Enzymatic activities were measured against collagen, keratin, elastin, and epidermis while a dehairing assay was performed by employing cowhide. All extracts showed similar activity on collagen and epidermis, while Bh and Pm were the most active against keratin at the same caseinolytic unit (CU) values; Bh was the only extract active against elastin. Bb (1 CU/ml), Bh (1.5 CU/ml), and Pm (0.5 CU/ml) were able to depilate cowhide. Desirable characteristics of dehairing were observed for all extracts since hair pores did not show residual hair, grain surface was clean and intact, and collagen fiber bundles of dermis were not damaged. In conclusion, results here presented show that proteolytic extracts of Bromeliaceae species are promising eco-compatible tools for leather industry.
Fil: Errasti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
Fil: Caffini, Nestor Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
Materia
BROMELIACEAE
ENZYMATIC DEHAIRING
LEATHER
LIME AND SULFIDE-FREE DEHAIRING
PLANT PROTEASES
PROTEIN WASTE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85358
Acceder
id CONICETDig_8b3a556db130656e40f282df5949d767
oai_identifier_str oai:ri.conicet.gov.ar:11336/85358
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industryErrasti, María EugeniaCaffini, Nestor OscarLopez, Laura Maria IsabelBROMELIACEAEENZYMATIC DEHAIRINGLEATHERLIME AND SULFIDE-FREE DEHAIRINGPLANT PROTEASESPROTEIN WASTEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Most tanneries use high proportions of Na2S and CaO during the dehairing step, resulting in effluents of high alkalinity and large amounts of suspended solid, besides the risk of liberating the toxic H2S. Solid waste rich in protein is another environmental problem of tanneries. Enzymes are an interesting technological tool for industry due to their biodegradability, nontoxic nature, and nonpolluting effluent generation. In the leather industry, proteases have been chosen as a promising eco-friendly alternative to Na2S/CaO dehairing. Extracts with high proteolytic activity have been obtained from fruits of Bromeliaceae species: Bromelia balansae Mez (Bb), Bromelia hieronymi Mez (Bh), and Pseudananas macrodontes (Morr.) Harms (Pm). In this work, Bb, Bh, and Pm have been studied for application in the leather industry, focusing in their dehairing properties. Enzymatic activities were measured against collagen, keratin, elastin, and epidermis while a dehairing assay was performed by employing cowhide. All extracts showed similar activity on collagen and epidermis, while Bh and Pm were the most active against keratin at the same caseinolytic unit (CU) values; Bh was the only extract active against elastin. Bb (1 CU/ml), Bh (1.5 CU/ml), and Pm (0.5 CU/ml) were able to depilate cowhide. Desirable characteristics of dehairing were observed for all extracts since hair pores did not show residual hair, grain surface was clean and intact, and collagen fiber bundles of dermis were not damaged. In conclusion, results here presented show that proteolytic extracts of Bromeliaceae species are promising eco-compatible tools for leather industry.Fil: Errasti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; ArgentinaFil: Caffini, Nestor Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; ArgentinaFil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; ArgentinaSpringer Heidelberg2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85358Errasti, María Eugenia; Caffini, Nestor Oscar; Lopez, Laura Maria Isabel; Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry; Springer Heidelberg; Environmental Science and Pollution Research; 25; 22; 8-2018; 21459-214660944-13441614-7499CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11356-017-1096-6info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-017-1096-6info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:35:59Zoai:ri.conicet.gov.ar:11336/85358instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:35:59.637CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
title Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
spellingShingle Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
Errasti, María Eugenia
BROMELIACEAE
ENZYMATIC DEHAIRING
LEATHER
LIME AND SULFIDE-FREE DEHAIRING
PLANT PROTEASES
PROTEIN WASTE
title_short Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
title_full Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
title_fullStr Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
title_full_unstemmed Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
title_sort Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry
dc.creator.none.fl_str_mv Errasti, María Eugenia
Caffini, Nestor Oscar
Lopez, Laura Maria Isabel
author Errasti, María Eugenia
author_facet Errasti, María Eugenia
Caffini, Nestor Oscar
Lopez, Laura Maria Isabel
author_role author
author2 Caffini, Nestor Oscar
Lopez, Laura Maria Isabel
author2_role author
author
dc.subject.none.fl_str_mv BROMELIACEAE
ENZYMATIC DEHAIRING
LEATHER
LIME AND SULFIDE-FREE DEHAIRING
PLANT PROTEASES
PROTEIN WASTE
topic BROMELIACEAE
ENZYMATIC DEHAIRING
LEATHER
LIME AND SULFIDE-FREE DEHAIRING
PLANT PROTEASES
PROTEIN WASTE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Most tanneries use high proportions of Na2S and CaO during the dehairing step, resulting in effluents of high alkalinity and large amounts of suspended solid, besides the risk of liberating the toxic H2S. Solid waste rich in protein is another environmental problem of tanneries. Enzymes are an interesting technological tool for industry due to their biodegradability, nontoxic nature, and nonpolluting effluent generation. In the leather industry, proteases have been chosen as a promising eco-friendly alternative to Na2S/CaO dehairing. Extracts with high proteolytic activity have been obtained from fruits of Bromeliaceae species: Bromelia balansae Mez (Bb), Bromelia hieronymi Mez (Bh), and Pseudananas macrodontes (Morr.) Harms (Pm). In this work, Bb, Bh, and Pm have been studied for application in the leather industry, focusing in their dehairing properties. Enzymatic activities were measured against collagen, keratin, elastin, and epidermis while a dehairing assay was performed by employing cowhide. All extracts showed similar activity on collagen and epidermis, while Bh and Pm were the most active against keratin at the same caseinolytic unit (CU) values; Bh was the only extract active against elastin. Bb (1 CU/ml), Bh (1.5 CU/ml), and Pm (0.5 CU/ml) were able to depilate cowhide. Desirable characteristics of dehairing were observed for all extracts since hair pores did not show residual hair, grain surface was clean and intact, and collagen fiber bundles of dermis were not damaged. In conclusion, results here presented show that proteolytic extracts of Bromeliaceae species are promising eco-compatible tools for leather industry.
Fil: Errasti, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
Fil: Caffini, Nestor Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina
Fil: Lopez, Laura Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina
description Most tanneries use high proportions of Na2S and CaO during the dehairing step, resulting in effluents of high alkalinity and large amounts of suspended solid, besides the risk of liberating the toxic H2S. Solid waste rich in protein is another environmental problem of tanneries. Enzymes are an interesting technological tool for industry due to their biodegradability, nontoxic nature, and nonpolluting effluent generation. In the leather industry, proteases have been chosen as a promising eco-friendly alternative to Na2S/CaO dehairing. Extracts with high proteolytic activity have been obtained from fruits of Bromeliaceae species: Bromelia balansae Mez (Bb), Bromelia hieronymi Mez (Bh), and Pseudananas macrodontes (Morr.) Harms (Pm). In this work, Bb, Bh, and Pm have been studied for application in the leather industry, focusing in their dehairing properties. Enzymatic activities were measured against collagen, keratin, elastin, and epidermis while a dehairing assay was performed by employing cowhide. All extracts showed similar activity on collagen and epidermis, while Bh and Pm were the most active against keratin at the same caseinolytic unit (CU) values; Bh was the only extract active against elastin. Bb (1 CU/ml), Bh (1.5 CU/ml), and Pm (0.5 CU/ml) were able to depilate cowhide. Desirable characteristics of dehairing were observed for all extracts since hair pores did not show residual hair, grain surface was clean and intact, and collagen fiber bundles of dermis were not damaged. In conclusion, results here presented show that proteolytic extracts of Bromeliaceae species are promising eco-compatible tools for leather industry.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85358
Errasti, María Eugenia; Caffini, Nestor Oscar; Lopez, Laura Maria Isabel; Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry; Springer Heidelberg; Environmental Science and Pollution Research; 25; 22; 8-2018; 21459-21466
0944-1344
1614-7499
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85358
identifier_str_mv Errasti, María Eugenia; Caffini, Nestor Oscar; Lopez, Laura Maria Isabel; Proteolytic extracts of three Bromeliaceae species as eco-compatible tools for leather industry; Springer Heidelberg; Environmental Science and Pollution Research; 25; 22; 8-2018; 21459-21466
0944-1344
1614-7499
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11356-017-1096-6
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11356-017-1096-6
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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