Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA

Autores
Oyhenart, Jorge; Morero, Mariana Paula
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection.
Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Morero, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Materia
CULTURE
DIAGNOSTIC
DNA
INSTRUMENT
LAMP
PCR
TRICHOMONAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213259
Acceder
id CONICETDig_8b38a5136b8c4a59e8fc86ac40133fda
oai_identifier_str oai:ri.conicet.gov.ar:11336/213259
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDAOyhenart, JorgeMorero, Mariana PaulaCULTUREDIAGNOSTICDNAINSTRUMENTLAMPPCRTRICHOMONAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection.Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Morero, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaSpringer2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213259Oyhenart, Jorge; Morero, Mariana Paula; Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA; Springer; Archives of Microbiology; 204; 3; 3-2022; 1-50302-8933CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s00203-022-02777-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-022-02777-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:36:15Zoai:ri.conicet.gov.ar:11336/213259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:36:15.598CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
title Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
spellingShingle Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
Oyhenart, Jorge
CULTURE
DIAGNOSTIC
DNA
INSTRUMENT
LAMP
PCR
TRICHOMONAS
title_short Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
title_full Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
title_fullStr Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
title_full_unstemmed Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
title_sort Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
dc.creator.none.fl_str_mv Oyhenart, Jorge
Morero, Mariana Paula
author Oyhenart, Jorge
author_facet Oyhenart, Jorge
Morero, Mariana Paula
author_role author
author2 Morero, Mariana Paula
author2_role author
dc.subject.none.fl_str_mv CULTURE
DIAGNOSTIC
DNA
INSTRUMENT
LAMP
PCR
TRICHOMONAS
topic CULTURE
DIAGNOSTIC
DNA
INSTRUMENT
LAMP
PCR
TRICHOMONAS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection.
Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Morero, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
description An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection.
publishDate 2022
dc.date.none.fl_str_mv 2022-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213259
Oyhenart, Jorge; Morero, Mariana Paula; Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA; Springer; Archives of Microbiology; 204; 3; 3-2022; 1-5
0302-8933
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213259
identifier_str_mv Oyhenart, Jorge; Morero, Mariana Paula; Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA; Springer; Archives of Microbiology; 204; 3; 3-2022; 1-5
0302-8933
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s00203-022-02777-1
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-022-02777-1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782002855936000
score 12.982451

Publicaciones similares