Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA
- Autores
- Oyhenart, Jorge; Morero, Mariana Paula
- Año de publicación
- 2022
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection.
Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Morero, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina - Materia
-
CULTURE
DIAGNOSTIC
DNA
INSTRUMENT
LAMP
PCR
TRICHOMONAS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/213259
Ver los metadatos del registro completo
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Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDAOyhenart, JorgeMorero, Mariana PaulaCULTUREDIAGNOSTICDNAINSTRUMENTLAMPPCRTRICHOMONAShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection.Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Morero, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaSpringer2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213259Oyhenart, Jorge; Morero, Mariana Paula; Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA; Springer; Archives of Microbiology; 204; 3; 3-2022; 1-50302-8933CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s00203-022-02777-1info:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-022-02777-1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:25Zoai:ri.conicet.gov.ar:11336/213259instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:25.519CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
title |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
spellingShingle |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA Oyhenart, Jorge CULTURE DIAGNOSTIC DNA INSTRUMENT LAMP PCR TRICHOMONAS |
title_short |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
title_full |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
title_fullStr |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
title_full_unstemmed |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
title_sort |
Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA |
dc.creator.none.fl_str_mv |
Oyhenart, Jorge Morero, Mariana Paula |
author |
Oyhenart, Jorge |
author_facet |
Oyhenart, Jorge Morero, Mariana Paula |
author_role |
author |
author2 |
Morero, Mariana Paula |
author2_role |
author |
dc.subject.none.fl_str_mv |
CULTURE DIAGNOSTIC DNA INSTRUMENT LAMP PCR TRICHOMONAS |
topic |
CULTURE DIAGNOSTIC DNA INSTRUMENT LAMP PCR TRICHOMONAS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection. Fil: Oyhenart, Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Morero, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina |
description |
An affordable, portable, and easy-to-build instrument was implemented to perform loop-mediated isothermal amplification (LAMP) in the field. Controlled by Arduino, CELDA heats the reaction tubes, reads the intensity of green fluorescent light and stores data in a computer. In the laboratory and in the field, CELDA was successfully used for the detection of Tritrichomonas foetus DNA in less than 60 min and helped distinguish positives with less difficulty than visual inspection. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/213259 Oyhenart, Jorge; Morero, Mariana Paula; Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA; Springer; Archives of Microbiology; 204; 3; 3-2022; 1-5 0302-8933 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/213259 |
identifier_str_mv |
Oyhenart, Jorge; Morero, Mariana Paula; Field detection of Tritrichomonas foetus through loop mediated isothermal amplification with CELDA; Springer; Archives of Microbiology; 204; 3; 3-2022; 1-5 0302-8933 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/10.1007/s00203-022-02777-1 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00203-022-02777-1 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |