Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area

Autores
Villegas, Liliana Beatriz; Amoroso, Maria Julia del R.; Abate, Carlos Mauricio
Año de publicación
2007
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Heavy metals can induce the formation of reactive oxygen species in the cell due to its redox properties. These forms of oxygen damage the cellular constituents causing oxidative stress. However, these molecules are detoxified by superoxide dismutases (SOD) and catalases (CAT). The aim of this work was to study the Cu(II) or Cr(VI) effect on the SOD and CAT activities in two actinomycete strains. . ABO and . MC1, isolated from contaminated sediments by heavy metals. The microorganisms were incubated in Minimal Medium-glucose supplemented with 10 and 20 ppm Cu(II)/Cr(VI). SOD activity was detected at 24, 48 and 72 h of incubation for native polyacrylamide gel electrophoresis and activity staining. The catalase activity was measured spectrophotometricaly by absorption decreasing at 240nm. Cells incubated in the absence of heavy metals were used as control. Cu(II) and Cr(VI) concentrations enhanced the total SOD and CAT activities from both strains and they increased with incubation time. While . ABO presented two bands, . MC1 showed a single band with SOD activity. One of the band from ABO and the band from . MC1 were sensitive to H O ; thus, they correspond to FeZnSOD. The second band from . ABO were not inhibited neither with nor N N , corresponding to MnSOD.
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLIII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
Mar del Plata
Argentina
Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
Materia
OXIDATIVE STRESS
COPPER
CHROMIUM
ACTINOMYCETES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195044
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted areaVillegas, Liliana BeatrizAmoroso, Maria Julia del R.Abate, Carlos MauricioOXIDATIVE STRESSCOPPERCHROMIUMACTINOMYCETEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Heavy metals can induce the formation of reactive oxygen species in the cell due to its redox properties. These forms of oxygen damage the cellular constituents causing oxidative stress. However, these molecules are detoxified by superoxide dismutases (SOD) and catalases (CAT). The aim of this work was to study the Cu(II) or Cr(VI) effect on the SOD and CAT activities in two actinomycete strains. . ABO and . MC1, isolated from contaminated sediments by heavy metals. The microorganisms were incubated in Minimal Medium-glucose supplemented with 10 and 20 ppm Cu(II)/Cr(VI). SOD activity was detected at 24, 48 and 72 h of incubation for native polyacrylamide gel electrophoresis and activity staining. The catalase activity was measured spectrophotometricaly by absorption decreasing at 240nm. Cells incubated in the absence of heavy metals were used as control. Cu(II) and Cr(VI) concentrations enhanced the total SOD and CAT activities from both strains and they increased with incubation time. While . ABO presented two bands, . MC1 showed a single band with SOD activity. One of the band from ABO and the band from . MC1 were sensitive to H O ; thus, they correspond to FeZnSOD. The second band from . ABO were not inhibited neither with nor N N , corresponding to MnSOD.Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLIII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y MolecularesMar del PlataArgentinaSociedad Argentina de Investigaciones Bioquímicas y MolecularesSociedad Argentina de Investigaciones Bioquímicas y Moleculares2007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195044Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area; XLIII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Moleculares; Mar del Plata; Argentina; 2007; 1730327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-31.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:30Zoai:ri.conicet.gov.ar:11336/195044instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:31.211CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
title Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
spellingShingle Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
Villegas, Liliana Beatriz
OXIDATIVE STRESS
COPPER
CHROMIUM
ACTINOMYCETES
title_short Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
title_full Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
title_fullStr Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
title_full_unstemmed Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
title_sort Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area
dc.creator.none.fl_str_mv Villegas, Liliana Beatriz
Amoroso, Maria Julia del R.
Abate, Carlos Mauricio
author Villegas, Liliana Beatriz
author_facet Villegas, Liliana Beatriz
Amoroso, Maria Julia del R.
Abate, Carlos Mauricio
author_role author
author2 Amoroso, Maria Julia del R.
Abate, Carlos Mauricio
author2_role author
author
dc.subject.none.fl_str_mv OXIDATIVE STRESS
COPPER
CHROMIUM
ACTINOMYCETES
topic OXIDATIVE STRESS
COPPER
CHROMIUM
ACTINOMYCETES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Heavy metals can induce the formation of reactive oxygen species in the cell due to its redox properties. These forms of oxygen damage the cellular constituents causing oxidative stress. However, these molecules are detoxified by superoxide dismutases (SOD) and catalases (CAT). The aim of this work was to study the Cu(II) or Cr(VI) effect on the SOD and CAT activities in two actinomycete strains. . ABO and . MC1, isolated from contaminated sediments by heavy metals. The microorganisms were incubated in Minimal Medium-glucose supplemented with 10 and 20 ppm Cu(II)/Cr(VI). SOD activity was detected at 24, 48 and 72 h of incubation for native polyacrylamide gel electrophoresis and activity staining. The catalase activity was measured spectrophotometricaly by absorption decreasing at 240nm. Cells incubated in the absence of heavy metals were used as control. Cu(II) and Cr(VI) concentrations enhanced the total SOD and CAT activities from both strains and they increased with incubation time. While . ABO presented two bands, . MC1 showed a single band with SOD activity. One of the band from ABO and the band from . MC1 were sensitive to H O ; thus, they correspond to FeZnSOD. The second band from . ABO were not inhibited neither with nor N N , corresponding to MnSOD.
Fil: Villegas, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Abate, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLIII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
Mar del Plata
Argentina
Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
description Heavy metals can induce the formation of reactive oxygen species in the cell due to its redox properties. These forms of oxygen damage the cellular constituents causing oxidative stress. However, these molecules are detoxified by superoxide dismutases (SOD) and catalases (CAT). The aim of this work was to study the Cu(II) or Cr(VI) effect on the SOD and CAT activities in two actinomycete strains. . ABO and . MC1, isolated from contaminated sediments by heavy metals. The microorganisms were incubated in Minimal Medium-glucose supplemented with 10 and 20 ppm Cu(II)/Cr(VI). SOD activity was detected at 24, 48 and 72 h of incubation for native polyacrylamide gel electrophoresis and activity staining. The catalase activity was measured spectrophotometricaly by absorption decreasing at 240nm. Cells incubated in the absence of heavy metals were used as control. Cu(II) and Cr(VI) concentrations enhanced the total SOD and CAT activities from both strains and they increased with incubation time. While . ABO presented two bands, . MC1 showed a single band with SOD activity. One of the band from ABO and the band from . MC1 were sensitive to H O ; thus, they correspond to FeZnSOD. The second band from . ABO were not inhibited neither with nor N N , corresponding to MnSOD.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195044
Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area; XLIII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Moleculares; Mar del Plata; Argentina; 2007; 173
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195044
identifier_str_mv Effect of Cu(II) and Cr(VI) on oxidative stress actinomycete strains isolated from polluted area; XLIII Reunión Anual de la Sociedad Argentina de Investigaciones Bioquímicas y Moleculares; Mar del Plata; Argentina; 2007; 173
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-31.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
publisher.none.fl_str_mv Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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