Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age

Autores
Schaumburg, Laura Gisela; Poletta, Gisela Laura; Siroski, Pablo Ariel; Mudry, Marta Dolores
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several studies indicate that certain factors such as age, sex or nutritional status among others, mayaffect the level of DNA damage, both induced and spontaneous, so it is very important to consider themfor a more accurate interpretation of the findings. The aim of this study was to analyze the influenceof age, sex, and nest of origin on spontaneous genetic damage of Tupinambis merianae determined bythe comet assay (CA) and the micronucleus (MN) test, in order to improve reference data for futurein vivo studies of xenobiotics exposure in this species. Sixty-five tegu lizards of three different ages:newborns (NB), juveniles (JUV) and adults (AD), both sexes and from different nests of origin were used.Blood samples were collected from the caudal vein of all animals and the MN test and CA were appliedon peripheral blood erythrocytes to determine basal frequency of MN (BFMN) and basal damage index(BDI). The comparison between age groups showed statistically significant differences in the BFMN andBDI (p < 0.05). NB animals showed significantly higher BDI values in relation to JUV and AD (p < 0.016), butno statistically differences were found between the latter two. NB showed lower BFMN respect to otherage groups, being statistically significant only when compared to AD (p < 0.016). BFMN or BDI showed nostatistically significant differences between sexes or nests of origin (p > 0.05). A weak negative relationshipwas found only between BFMN and weight of NB tegu lizard (p = 0.014; R2= 0.245). Basal values of geneticdamage obtained with both biomarkers in the tegu lizard evidenced that age is an intrinsic factor thatshould be taken into account to avoid misunderstanding of the results in future biomonitoring studies.
Fil: Schaumburg, Laura Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
Materia
Basal Genetic Damage
Comet Assay
Micronucleus Test
Age
Monitoring
Tegu Lizard
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20297
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20297
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of ageSchaumburg, Laura GiselaPoletta, Gisela LauraSiroski, Pablo ArielMudry, Marta DoloresBasal Genetic DamageComet AssayMicronucleus TestAgeMonitoringTegu Lizardhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several studies indicate that certain factors such as age, sex or nutritional status among others, mayaffect the level of DNA damage, both induced and spontaneous, so it is very important to consider themfor a more accurate interpretation of the findings. The aim of this study was to analyze the influenceof age, sex, and nest of origin on spontaneous genetic damage of Tupinambis merianae determined bythe comet assay (CA) and the micronucleus (MN) test, in order to improve reference data for futurein vivo studies of xenobiotics exposure in this species. Sixty-five tegu lizards of three different ages:newborns (NB), juveniles (JUV) and adults (AD), both sexes and from different nests of origin were used.Blood samples were collected from the caudal vein of all animals and the MN test and CA were appliedon peripheral blood erythrocytes to determine basal frequency of MN (BFMN) and basal damage index(BDI). The comparison between age groups showed statistically significant differences in the BFMN andBDI (p < 0.05). NB animals showed significantly higher BDI values in relation to JUV and AD (p < 0.016), butno statistically differences were found between the latter two. NB showed lower BFMN respect to otherage groups, being statistically significant only when compared to AD (p < 0.016). BFMN or BDI showed nostatistically significant differences between sexes or nests of origin (p > 0.05). A weak negative relationshipwas found only between BFMN and weight of NB tegu lizard (p = 0.014; R2= 0.245). Basal values of geneticdamage obtained with both biomarkers in the tegu lizard evidenced that age is an intrinsic factor thatshould be taken into account to avoid misunderstanding of the results in future biomonitoring studies.Fil: Schaumburg, Laura Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); ArgentinaFil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); ArgentinaFil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaElsevier2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20297Schaumburg, Laura Gisela; Poletta, Gisela Laura; Siroski, Pablo Ariel; Mudry, Marta Dolores; Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age; Elsevier; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 766; 5-2014; 5-91383-5718CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2014.03.007info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383571814000849info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:42Zoai:ri.conicet.gov.ar:11336/20297instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:42.832CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
spellingShingle Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
Schaumburg, Laura Gisela
Basal Genetic Damage
Comet Assay
Micronucleus Test
Age
Monitoring
Tegu Lizard
title_short Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_full Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_fullStr Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_full_unstemmed Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
title_sort Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age
dc.creator.none.fl_str_mv Schaumburg, Laura Gisela
Poletta, Gisela Laura
Siroski, Pablo Ariel
Mudry, Marta Dolores
author Schaumburg, Laura Gisela
author_facet Schaumburg, Laura Gisela
Poletta, Gisela Laura
Siroski, Pablo Ariel
Mudry, Marta Dolores
author_role author
author2 Poletta, Gisela Laura
Siroski, Pablo Ariel
Mudry, Marta Dolores
author2_role author
author
author
dc.subject.none.fl_str_mv Basal Genetic Damage
Comet Assay
Micronucleus Test
Age
Monitoring
Tegu Lizard
topic Basal Genetic Damage
Comet Assay
Micronucleus Test
Age
Monitoring
Tegu Lizard
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several studies indicate that certain factors such as age, sex or nutritional status among others, mayaffect the level of DNA damage, both induced and spontaneous, so it is very important to consider themfor a more accurate interpretation of the findings. The aim of this study was to analyze the influenceof age, sex, and nest of origin on spontaneous genetic damage of Tupinambis merianae determined bythe comet assay (CA) and the micronucleus (MN) test, in order to improve reference data for futurein vivo studies of xenobiotics exposure in this species. Sixty-five tegu lizards of three different ages:newborns (NB), juveniles (JUV) and adults (AD), both sexes and from different nests of origin were used.Blood samples were collected from the caudal vein of all animals and the MN test and CA were appliedon peripheral blood erythrocytes to determine basal frequency of MN (BFMN) and basal damage index(BDI). The comparison between age groups showed statistically significant differences in the BFMN andBDI (p < 0.05). NB animals showed significantly higher BDI values in relation to JUV and AD (p < 0.016), butno statistically differences were found between the latter two. NB showed lower BFMN respect to otherage groups, being statistically significant only when compared to AD (p < 0.016). BFMN or BDI showed nostatistically significant differences between sexes or nests of origin (p > 0.05). A weak negative relationshipwas found only between BFMN and weight of NB tegu lizard (p = 0.014; R2= 0.245). Basal values of geneticdamage obtained with both biomarkers in the tegu lizard evidenced that age is an intrinsic factor thatshould be taken into account to avoid misunderstanding of the results in future biomonitoring studies.
Fil: Schaumburg, Laura Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Fil: Poletta, Gisela Laura. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Siroski, Pablo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina. Universidad Nacional del Litoral. Facultad de Humanidades y Ciencias. Departamento de Ciencias Naturales. Laboratorio de Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA); Argentina
Fil: Mudry, Marta Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina
description Several studies indicate that certain factors such as age, sex or nutritional status among others, mayaffect the level of DNA damage, both induced and spontaneous, so it is very important to consider themfor a more accurate interpretation of the findings. The aim of this study was to analyze the influenceof age, sex, and nest of origin on spontaneous genetic damage of Tupinambis merianae determined bythe comet assay (CA) and the micronucleus (MN) test, in order to improve reference data for futurein vivo studies of xenobiotics exposure in this species. Sixty-five tegu lizards of three different ages:newborns (NB), juveniles (JUV) and adults (AD), both sexes and from different nests of origin were used.Blood samples were collected from the caudal vein of all animals and the MN test and CA were appliedon peripheral blood erythrocytes to determine basal frequency of MN (BFMN) and basal damage index(BDI). The comparison between age groups showed statistically significant differences in the BFMN andBDI (p < 0.05). NB animals showed significantly higher BDI values in relation to JUV and AD (p < 0.016), butno statistically differences were found between the latter two. NB showed lower BFMN respect to otherage groups, being statistically significant only when compared to AD (p < 0.016). BFMN or BDI showed nostatistically significant differences between sexes or nests of origin (p > 0.05). A weak negative relationshipwas found only between BFMN and weight of NB tegu lizard (p = 0.014; R2= 0.245). Basal values of geneticdamage obtained with both biomarkers in the tegu lizard evidenced that age is an intrinsic factor thatshould be taken into account to avoid misunderstanding of the results in future biomonitoring studies.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20297
Schaumburg, Laura Gisela; Poletta, Gisela Laura; Siroski, Pablo Ariel; Mudry, Marta Dolores; Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age; Elsevier; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 766; 5-2014; 5-9
1383-5718
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20297
identifier_str_mv Schaumburg, Laura Gisela; Poletta, Gisela Laura; Siroski, Pablo Ariel; Mudry, Marta Dolores; Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): The effect of age; Elsevier; Mutation Research. Genetic Toxicology And Environmental Mutagenesis; 766; 5-2014; 5-9
1383-5718
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mrgentox.2014.03.007
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1383571814000849
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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