Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines

Autores
Vela Gurovic, Maria Soledad; Díaz Lanza, A. María; Boyano Adánez, María Del Carmen; Estañ Omaña, M. Cristina; Gañán Gõmez, Irene; Murray, Ana Paula; Sancho Lõpez, Pilar
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Natural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC 50 values of 1.98 ± 0.12 μm and 3.52 ± 0.14 μm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (Δψm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of Δψm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment. Copyright © 2010 John Wiley & Sons, Ltd.
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad de Alcalá; España
Fil: Díaz Lanza, A. María. Universidad de Alcalá; España
Fil: Boyano Adánez, María Del Carmen. Universidad de Alcalá; España
Fil: Estañ Omaña, M. Cristina. Universidad de Alcalá; España
Fil: Gañán Gõmez, Irene. Universidad de Alcalá; España
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Sancho Lõpez, Pilar. Universidad de Alcalá; España
Materia
Apoptosis
Dequalinium
Heliantriol B2
K562 Cell Line
Leukemia
Nb4 Cell Line
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64229
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64229
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell linesVela Gurovic, Maria SoledadDíaz Lanza, A. MaríaBoyano Adánez, María Del CarmenEstañ Omaña, M. CristinaGañán Gõmez, IreneMurray, Ana PaulaSancho Lõpez, PilarApoptosisDequaliniumHeliantriol B2K562 Cell LineLeukemiaNb4 Cell Linehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Natural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC 50 values of 1.98 ± 0.12 μm and 3.52 ± 0.14 μm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (Δψm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of Δψm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment. Copyright © 2010 John Wiley & Sons, Ltd.Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad de Alcalá; EspañaFil: Díaz Lanza, A. María. Universidad de Alcalá; EspañaFil: Boyano Adánez, María Del Carmen. Universidad de Alcalá; EspañaFil: Estañ Omaña, M. Cristina. Universidad de Alcalá; EspañaFil: Gañán Gõmez, Irene. Universidad de Alcalá; EspañaFil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Sancho Lõpez, Pilar. Universidad de Alcalá; EspañaJohn Wiley & Sons Ltd2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64229Vela Gurovic, Maria Soledad; Díaz Lanza, A. María; Boyano Adánez, María Del Carmen; Estañ Omaña, M. Cristina; Gañán Gõmez, Irene; et al.; Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines; John Wiley & Sons Ltd; Phytotherapy Research; 25; 4; 4-2011; 603-6100951-418XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/ptr.3310info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.3310info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:27Zoai:ri.conicet.gov.ar:11336/64229instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:27.857CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
title Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
spellingShingle Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
Vela Gurovic, Maria Soledad
Apoptosis
Dequalinium
Heliantriol B2
K562 Cell Line
Leukemia
Nb4 Cell Line
title_short Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
title_full Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
title_fullStr Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
title_full_unstemmed Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
title_sort Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
dc.creator.none.fl_str_mv Vela Gurovic, Maria Soledad
Díaz Lanza, A. María
Boyano Adánez, María Del Carmen
Estañ Omaña, M. Cristina
Gañán Gõmez, Irene
Murray, Ana Paula
Sancho Lõpez, Pilar
author Vela Gurovic, Maria Soledad
author_facet Vela Gurovic, Maria Soledad
Díaz Lanza, A. María
Boyano Adánez, María Del Carmen
Estañ Omaña, M. Cristina
Gañán Gõmez, Irene
Murray, Ana Paula
Sancho Lõpez, Pilar
author_role author
author2 Díaz Lanza, A. María
Boyano Adánez, María Del Carmen
Estañ Omaña, M. Cristina
Gañán Gõmez, Irene
Murray, Ana Paula
Sancho Lõpez, Pilar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Dequalinium
Heliantriol B2
K562 Cell Line
Leukemia
Nb4 Cell Line
topic Apoptosis
Dequalinium
Heliantriol B2
K562 Cell Line
Leukemia
Nb4 Cell Line
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Natural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC 50 values of 1.98 ± 0.12 μm and 3.52 ± 0.14 μm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (Δψm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of Δψm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment. Copyright © 2010 John Wiley & Sons, Ltd.
Fil: Vela Gurovic, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad de Alcalá; España
Fil: Díaz Lanza, A. María. Universidad de Alcalá; España
Fil: Boyano Adánez, María Del Carmen. Universidad de Alcalá; España
Fil: Estañ Omaña, M. Cristina. Universidad de Alcalá; España
Fil: Gañán Gõmez, Irene. Universidad de Alcalá; España
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Sancho Lõpez, Pilar. Universidad de Alcalá; España
description Natural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC 50 values of 1.98 ± 0.12 μm and 3.52 ± 0.14 μm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (Δψm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of Δψm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment. Copyright © 2010 John Wiley & Sons, Ltd.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64229
Vela Gurovic, Maria Soledad; Díaz Lanza, A. María; Boyano Adánez, María Del Carmen; Estañ Omaña, M. Cristina; Gañán Gõmez, Irene; et al.; Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines; John Wiley & Sons Ltd; Phytotherapy Research; 25; 4; 4-2011; 603-610
0951-418X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64229
identifier_str_mv Vela Gurovic, Maria Soledad; Díaz Lanza, A. María; Boyano Adánez, María Del Carmen; Estañ Omaña, M. Cristina; Gañán Gõmez, Irene; et al.; Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines; John Wiley & Sons Ltd; Phytotherapy Research; 25; 4; 4-2011; 603-610
0951-418X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/ptr.3310
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/ptr.3310
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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