Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina

Autores
Quintas, Pamela Yanina; Keunchkarian, Sonia; Romero, Lilian; Canizo, Brenda Vanina; Wuilloud, Rodolfo German; Castells, Cecilia Beatriz Marta
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The amino acid (AA) content in honeys from Mendoza (Argentina) was determined by high-performance liquid chromatography and the relative quantities of D- and L-AAs were determined by chiral gas chromatography coupled to mass spectrometry. The results showed that proline was the most abundant AA in all analyzed samples, followed by phenylalanine. Based on the AA content, different chemometric tools were assessed for provenance differentiation. The unsupervised chemometric methods, however, could not differentiate unquestionably the geographical origin of honey based only on their AA content. Enantiomeric ratio demonstrated that D-proline amount was lower than D-phenylalanine levels in practically all honey samples. In addition, D-enantiomers of alanine, valine, glutamic acid, leucine, and isoleucine were found in most samples. The study demonstrated that certain D-AAs can occur naturally in this foodstuff, probably, as a consequence of the Maillard reaction, which is not dependent on microorganism actions. Novelty impact statement: The amino acid content in honey samples from Mendoza was determined by high-performance liquid chromatography. Proline and phenylalanine were the more abundant amino acid. Amino acid enantiomeric ratio was assessed by chiral gas chromatography coupled to mass spectrometry. D-amino acids can occur naturally in the honey samples from Mendoza (Argentina). Chemometric tools were applied to discriminate samples from the geographical origin.
Fil: Quintas, Pamela Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina
Fil: Keunchkarian, Sonia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Fil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Fil: Canizo, Brenda Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina
Fil: Wuilloud, Rodolfo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Materia
AMINOACIDS
CHROMATOGRAPHY
ENANTIOMERIC RATIO
CHEMOMETRICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/148882
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/148882
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, ArgentinaQuintas, Pamela YaninaKeunchkarian, SoniaRomero, LilianCanizo, Brenda VaninaWuilloud, Rodolfo GermanCastells, Cecilia Beatriz MartaAMINOACIDSCHROMATOGRAPHYENANTIOMERIC RATIOCHEMOMETRICShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The amino acid (AA) content in honeys from Mendoza (Argentina) was determined by high-performance liquid chromatography and the relative quantities of D- and L-AAs were determined by chiral gas chromatography coupled to mass spectrometry. The results showed that proline was the most abundant AA in all analyzed samples, followed by phenylalanine. Based on the AA content, different chemometric tools were assessed for provenance differentiation. The unsupervised chemometric methods, however, could not differentiate unquestionably the geographical origin of honey based only on their AA content. Enantiomeric ratio demonstrated that D-proline amount was lower than D-phenylalanine levels in practically all honey samples. In addition, D-enantiomers of alanine, valine, glutamic acid, leucine, and isoleucine were found in most samples. The study demonstrated that certain D-AAs can occur naturally in this foodstuff, probably, as a consequence of the Maillard reaction, which is not dependent on microorganism actions. Novelty impact statement: The amino acid content in honey samples from Mendoza was determined by high-performance liquid chromatography. Proline and phenylalanine were the more abundant amino acid. Amino acid enantiomeric ratio was assessed by chiral gas chromatography coupled to mass spectrometry. D-amino acids can occur naturally in the honey samples from Mendoza (Argentina). Chemometric tools were applied to discriminate samples from the geographical origin.Fil: Quintas, Pamela Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; ArgentinaFil: Keunchkarian, Sonia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; ArgentinaFil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; ArgentinaFil: Canizo, Brenda Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; ArgentinaFil: Wuilloud, Rodolfo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; ArgentinaFil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; ArgentinaWiley Blackwell Publishing, Inc2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/148882Quintas, Pamela Yanina; Keunchkarian, Sonia; Romero, Lilian; Canizo, Brenda Vanina; Wuilloud, Rodolfo German; et al.; Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina; Wiley Blackwell Publishing, Inc; Journal of Food Processing and Preservation; 45; 12; 9-2021; 1-110145-8892CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jfpp.15966info:eu-repo/semantics/altIdentifier/url/https://ifst.onlinelibrary.wiley.com/doi/10.1111/jfpp.15966info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:42:44Zoai:ri.conicet.gov.ar:11336/148882instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:42:44.587CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
title Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
spellingShingle Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
Quintas, Pamela Yanina
AMINOACIDS
CHROMATOGRAPHY
ENANTIOMERIC RATIO
CHEMOMETRICS
title_short Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
title_full Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
title_fullStr Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
title_full_unstemmed Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
title_sort Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina
dc.creator.none.fl_str_mv Quintas, Pamela Yanina
Keunchkarian, Sonia
Romero, Lilian
Canizo, Brenda Vanina
Wuilloud, Rodolfo German
Castells, Cecilia Beatriz Marta
author Quintas, Pamela Yanina
author_facet Quintas, Pamela Yanina
Keunchkarian, Sonia
Romero, Lilian
Canizo, Brenda Vanina
Wuilloud, Rodolfo German
Castells, Cecilia Beatriz Marta
author_role author
author2 Keunchkarian, Sonia
Romero, Lilian
Canizo, Brenda Vanina
Wuilloud, Rodolfo German
Castells, Cecilia Beatriz Marta
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AMINOACIDS
CHROMATOGRAPHY
ENANTIOMERIC RATIO
CHEMOMETRICS
topic AMINOACIDS
CHROMATOGRAPHY
ENANTIOMERIC RATIO
CHEMOMETRICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The amino acid (AA) content in honeys from Mendoza (Argentina) was determined by high-performance liquid chromatography and the relative quantities of D- and L-AAs were determined by chiral gas chromatography coupled to mass spectrometry. The results showed that proline was the most abundant AA in all analyzed samples, followed by phenylalanine. Based on the AA content, different chemometric tools were assessed for provenance differentiation. The unsupervised chemometric methods, however, could not differentiate unquestionably the geographical origin of honey based only on their AA content. Enantiomeric ratio demonstrated that D-proline amount was lower than D-phenylalanine levels in practically all honey samples. In addition, D-enantiomers of alanine, valine, glutamic acid, leucine, and isoleucine were found in most samples. The study demonstrated that certain D-AAs can occur naturally in this foodstuff, probably, as a consequence of the Maillard reaction, which is not dependent on microorganism actions. Novelty impact statement: The amino acid content in honey samples from Mendoza was determined by high-performance liquid chromatography. Proline and phenylalanine were the more abundant amino acid. Amino acid enantiomeric ratio was assessed by chiral gas chromatography coupled to mass spectrometry. D-amino acids can occur naturally in the honey samples from Mendoza (Argentina). Chemometric tools were applied to discriminate samples from the geographical origin.
Fil: Quintas, Pamela Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina
Fil: Keunchkarian, Sonia. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Fil: Romero, Lilian. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
Fil: Canizo, Brenda Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina
Fil: Wuilloud, Rodolfo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Interdisciplinario de Ciencias Básicas. - Universidad Nacional de Cuyo. Instituto Interdisciplinario de Ciencias Básicas; Argentina
Fil: Castells, Cecilia Beatriz Marta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Métodos Analíticos. - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires. Laboratorio de Investigación y Desarrollo de Métodos Analíticos; Argentina
description The amino acid (AA) content in honeys from Mendoza (Argentina) was determined by high-performance liquid chromatography and the relative quantities of D- and L-AAs were determined by chiral gas chromatography coupled to mass spectrometry. The results showed that proline was the most abundant AA in all analyzed samples, followed by phenylalanine. Based on the AA content, different chemometric tools were assessed for provenance differentiation. The unsupervised chemometric methods, however, could not differentiate unquestionably the geographical origin of honey based only on their AA content. Enantiomeric ratio demonstrated that D-proline amount was lower than D-phenylalanine levels in practically all honey samples. In addition, D-enantiomers of alanine, valine, glutamic acid, leucine, and isoleucine were found in most samples. The study demonstrated that certain D-AAs can occur naturally in this foodstuff, probably, as a consequence of the Maillard reaction, which is not dependent on microorganism actions. Novelty impact statement: The amino acid content in honey samples from Mendoza was determined by high-performance liquid chromatography. Proline and phenylalanine were the more abundant amino acid. Amino acid enantiomeric ratio was assessed by chiral gas chromatography coupled to mass spectrometry. D-amino acids can occur naturally in the honey samples from Mendoza (Argentina). Chemometric tools were applied to discriminate samples from the geographical origin.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/148882
Quintas, Pamela Yanina; Keunchkarian, Sonia; Romero, Lilian; Canizo, Brenda Vanina; Wuilloud, Rodolfo German; et al.; Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina; Wiley Blackwell Publishing, Inc; Journal of Food Processing and Preservation; 45; 12; 9-2021; 1-11
0145-8892
CONICET Digital
CONICET
url http://hdl.handle.net/11336/148882
identifier_str_mv Quintas, Pamela Yanina; Keunchkarian, Sonia; Romero, Lilian; Canizo, Brenda Vanina; Wuilloud, Rodolfo German; et al.; Determination of amino acid content and its enantiomeric composition in honey samples from Mendoza, Argentina; Wiley Blackwell Publishing, Inc; Journal of Food Processing and Preservation; 45; 12; 9-2021; 1-11
0145-8892
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jfpp.15966
info:eu-repo/semantics/altIdentifier/url/https://ifst.onlinelibrary.wiley.com/doi/10.1111/jfpp.15966
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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