Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease

Autores
Juiz, Natalia Anahí; Torrejón, Irma del Rosario; Burgos, Marianela; Torres, Ana María Fernanda; Duffy, Tomás; Cayo, Nelly Melina; Tabasco, Anahí; Salvo, Miriam; Longhi, Silvia Andrea; Schijman, Alejandro Gabriel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi infection in women of reproductive age is associated with congenital transmission and adverse pregnancy outcomes. The placenta is a key barrier to infection. Gene expression profiles of term placental environment from T. cruzi–seropositive (SP) and -seronegative (SN) mothers were characterized by RNA-Seq. Nine pools of placental RNA paired samples were used: three from SN and six from SP tissues. Each pool consisted of female/male newborns and vaginal/cesarean delivery binomials. No newborn was congenitally infected. T. cruzi satellite DNA quantitative PCR in placental tissues and maternal and neonatal blood, and parasite 18S quantitative RT-PCR from placental RNA were negative, except in three SP women's bloodstream. To identify pathways associated with maternal T. cruzi infection, a gene-set association analysis was implemented: SP placental samples showed overexpression of inflammatory response and lymphocytic activation, whereas numerous biosynthetic processes were down-regulated. About 42 genes showed a significant fold-change between SP and SN groups. KISS1 and CGB5 were down-regulated, whereas KIF12, HLA-G, PRG2, TAC3, FN1, and ATXN3L were up-regulated. Several expressed genes in SP placentas encode proteins associated with preeclampsia and miscarriage. This first transcriptomics study in human term placental environment shows a placental response that may affect the fetus while protecting it from parasite infection; this host response could be responsible for the low rate of congenital transmission in chronic Chagas disease.
Fil: Juiz, Natalia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Torrejón, Irma del Rosario. Universidad Nacional de Jujuy; Argentina
Fil: Burgos, Marianela. Hospital Nacional Professor Dr. Alejandro Posadas; Argentina
Fil: Torres, Ana María Fernanda. Universidad Nacional de Jujuy; Argentina
Fil: Duffy, Tomás. University Of Southern California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cayo, Nelly Melina. Universidad Nacional de Jujuy; Argentina
Fil: Tabasco, Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Salvo, Miriam. Hospital Nacional Professor Dr. Alejandro Posadas; Argentina
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
TRYPANOSOMA CRUZI
CHAGAS DISESASE
PLACENTAL GENE EXPRESSION
GENOMICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96271
Acceder
id CONICETDig_899c5768546cd70a8fa7083b76691ca2
oai_identifier_str oai:ri.conicet.gov.ar:11336/96271
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas DiseaseJuiz, Natalia AnahíTorrejón, Irma del RosarioBurgos, MarianelaTorres, Ana María FernandaDuffy, TomásCayo, Nelly MelinaTabasco, AnahíSalvo, MiriamLonghi, Silvia AndreaSchijman, Alejandro GabrielTRYPANOSOMA CRUZICHAGAS DISESASEPLACENTAL GENE EXPRESSIONGENOMICShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Trypanosoma cruzi infection in women of reproductive age is associated with congenital transmission and adverse pregnancy outcomes. The placenta is a key barrier to infection. Gene expression profiles of term placental environment from T. cruzi–seropositive (SP) and -seronegative (SN) mothers were characterized by RNA-Seq. Nine pools of placental RNA paired samples were used: three from SN and six from SP tissues. Each pool consisted of female/male newborns and vaginal/cesarean delivery binomials. No newborn was congenitally infected. T. cruzi satellite DNA quantitative PCR in placental tissues and maternal and neonatal blood, and parasite 18S quantitative RT-PCR from placental RNA were negative, except in three SP women's bloodstream. To identify pathways associated with maternal T. cruzi infection, a gene-set association analysis was implemented: SP placental samples showed overexpression of inflammatory response and lymphocytic activation, whereas numerous biosynthetic processes were down-regulated. About 42 genes showed a significant fold-change between SP and SN groups. KISS1 and CGB5 were down-regulated, whereas KIF12, HLA-G, PRG2, TAC3, FN1, and ATXN3L were up-regulated. Several expressed genes in SP placentas encode proteins associated with preeclampsia and miscarriage. This first transcriptomics study in human term placental environment shows a placental response that may affect the fetus while protecting it from parasite infection; this host response could be responsible for the low rate of congenital transmission in chronic Chagas disease.Fil: Juiz, Natalia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Torrejón, Irma del Rosario. Universidad Nacional de Jujuy; ArgentinaFil: Burgos, Marianela. Hospital Nacional Professor Dr. Alejandro Posadas; ArgentinaFil: Torres, Ana María Fernanda. Universidad Nacional de Jujuy; ArgentinaFil: Duffy, Tomás. University Of Southern California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cayo, Nelly Melina. Universidad Nacional de Jujuy; ArgentinaFil: Tabasco, Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Salvo, Miriam. Hospital Nacional Professor Dr. Alejandro Posadas; ArgentinaFil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaAmerican Society of Investigative Pathology2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96271Juiz, Natalia Anahí; Torrejón, Irma del Rosario; Burgos, Marianela; Torres, Ana María Fernanda; Duffy, Tomás; et al.; Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease; American Society of Investigative Pathology; American Journal Of Pathology; 188; 6; 6-2018; 1345-13530002-94401525-2191CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000294401830018Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ajpath.2018.02.011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:10Zoai:ri.conicet.gov.ar:11336/96271instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:10.473CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
title Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
spellingShingle Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
Juiz, Natalia Anahí
TRYPANOSOMA CRUZI
CHAGAS DISESASE
PLACENTAL GENE EXPRESSION
GENOMICS
title_short Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
title_full Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
title_fullStr Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
title_full_unstemmed Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
title_sort Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease
dc.creator.none.fl_str_mv Juiz, Natalia Anahí
Torrejón, Irma del Rosario
Burgos, Marianela
Torres, Ana María Fernanda
Duffy, Tomás
Cayo, Nelly Melina
Tabasco, Anahí
Salvo, Miriam
Longhi, Silvia Andrea
Schijman, Alejandro Gabriel
author Juiz, Natalia Anahí
author_facet Juiz, Natalia Anahí
Torrejón, Irma del Rosario
Burgos, Marianela
Torres, Ana María Fernanda
Duffy, Tomás
Cayo, Nelly Melina
Tabasco, Anahí
Salvo, Miriam
Longhi, Silvia Andrea
Schijman, Alejandro Gabriel
author_role author
author2 Torrejón, Irma del Rosario
Burgos, Marianela
Torres, Ana María Fernanda
Duffy, Tomás
Cayo, Nelly Melina
Tabasco, Anahí
Salvo, Miriam
Longhi, Silvia Andrea
Schijman, Alejandro Gabriel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
CHAGAS DISESASE
PLACENTAL GENE EXPRESSION
GENOMICS
topic TRYPANOSOMA CRUZI
CHAGAS DISESASE
PLACENTAL GENE EXPRESSION
GENOMICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Trypanosoma cruzi infection in women of reproductive age is associated with congenital transmission and adverse pregnancy outcomes. The placenta is a key barrier to infection. Gene expression profiles of term placental environment from T. cruzi–seropositive (SP) and -seronegative (SN) mothers were characterized by RNA-Seq. Nine pools of placental RNA paired samples were used: three from SN and six from SP tissues. Each pool consisted of female/male newborns and vaginal/cesarean delivery binomials. No newborn was congenitally infected. T. cruzi satellite DNA quantitative PCR in placental tissues and maternal and neonatal blood, and parasite 18S quantitative RT-PCR from placental RNA were negative, except in three SP women's bloodstream. To identify pathways associated with maternal T. cruzi infection, a gene-set association analysis was implemented: SP placental samples showed overexpression of inflammatory response and lymphocytic activation, whereas numerous biosynthetic processes were down-regulated. About 42 genes showed a significant fold-change between SP and SN groups. KISS1 and CGB5 were down-regulated, whereas KIF12, HLA-G, PRG2, TAC3, FN1, and ATXN3L were up-regulated. Several expressed genes in SP placentas encode proteins associated with preeclampsia and miscarriage. This first transcriptomics study in human term placental environment shows a placental response that may affect the fetus while protecting it from parasite infection; this host response could be responsible for the low rate of congenital transmission in chronic Chagas disease.
Fil: Juiz, Natalia Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Torrejón, Irma del Rosario. Universidad Nacional de Jujuy; Argentina
Fil: Burgos, Marianela. Hospital Nacional Professor Dr. Alejandro Posadas; Argentina
Fil: Torres, Ana María Fernanda. Universidad Nacional de Jujuy; Argentina
Fil: Duffy, Tomás. University Of Southern California; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cayo, Nelly Melina. Universidad Nacional de Jujuy; Argentina
Fil: Tabasco, Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Salvo, Miriam. Hospital Nacional Professor Dr. Alejandro Posadas; Argentina
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Schijman, Alejandro Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description Trypanosoma cruzi infection in women of reproductive age is associated with congenital transmission and adverse pregnancy outcomes. The placenta is a key barrier to infection. Gene expression profiles of term placental environment from T. cruzi–seropositive (SP) and -seronegative (SN) mothers were characterized by RNA-Seq. Nine pools of placental RNA paired samples were used: three from SN and six from SP tissues. Each pool consisted of female/male newborns and vaginal/cesarean delivery binomials. No newborn was congenitally infected. T. cruzi satellite DNA quantitative PCR in placental tissues and maternal and neonatal blood, and parasite 18S quantitative RT-PCR from placental RNA were negative, except in three SP women's bloodstream. To identify pathways associated with maternal T. cruzi infection, a gene-set association analysis was implemented: SP placental samples showed overexpression of inflammatory response and lymphocytic activation, whereas numerous biosynthetic processes were down-regulated. About 42 genes showed a significant fold-change between SP and SN groups. KISS1 and CGB5 were down-regulated, whereas KIF12, HLA-G, PRG2, TAC3, FN1, and ATXN3L were up-regulated. Several expressed genes in SP placentas encode proteins associated with preeclampsia and miscarriage. This first transcriptomics study in human term placental environment shows a placental response that may affect the fetus while protecting it from parasite infection; this host response could be responsible for the low rate of congenital transmission in chronic Chagas disease.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96271
Juiz, Natalia Anahí; Torrejón, Irma del Rosario; Burgos, Marianela; Torres, Ana María Fernanda; Duffy, Tomás; et al.; Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease; American Society of Investigative Pathology; American Journal Of Pathology; 188; 6; 6-2018; 1345-1353
0002-9440
1525-2191
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96271
identifier_str_mv Juiz, Natalia Anahí; Torrejón, Irma del Rosario; Burgos, Marianela; Torres, Ana María Fernanda; Duffy, Tomás; et al.; Alterations in Placental Gene Expression of Pregnant Women with Chronic Chagas Disease; American Society of Investigative Pathology; American Journal Of Pathology; 188; 6; 6-2018; 1345-1353
0002-9440
1525-2191
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S000294401830018X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ajpath.2018.02.011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society of Investigative Pathology
publisher.none.fl_str_mv American Society of Investigative Pathology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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