B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)

Autores
Ferro, Juan Martín; Marti, Dardo Andrea; Bidau, Claudio Juan; Suarez, Pablo; Nagamachi, Cleusa Y.; Pieczarka, Júlio C.; Baldo, Juan Diego
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Supernumerary or B chromosomes are one of the main causes for numerical chromosomal variation in higher eukaryotes. These extragenetic elements have been studied for more than a century, with the goal of trying to understand their origin, and how they survive as a polymorphism in natural populations. Hypsiboas albopunctatus is a nocturnal hylid frog distributed in the central–eastern part of South America. Previously, variation in chromosome numbers was described for a population from Rio Claro, São Paulo, Brazil, in which a single small-sized, metacentric B chromosome was present in ca. 40% of the analyzed individuals (n = 17). We herein describe the presence of B chromosomes in populations of H. albopunctatus from northeastern Argentina (Corrientes and Misiones provinces), with unusual morphological and structural characteristics. The frequency of B chromosomes varied significantly among analyzed populations. We found four diploid numbers (2N = 22, 22 + 1B, 22 + 2B, and 22 + 3B), and in a few individuals mitotic instability occurred. C banding revealed variations in the heterochromatin (DAPI+) pattern between Bs with similar morphology, indicating the existence of two new structural variants of these supernumerary elements in H. albopunctatus (B1 and B2). Nucleolar organizer regions marked positively on the eighth pair, coincident with the location of ribosomal DNA as demonstrated with fluorescent in situ hybridization, but Bs did not mark positive with these two techniques. Also, fluorescent in situ hybridization with telomeric probes showed no differences in location and intensity between Bs and autosomal chromosomes. The present communication is the first case of B chromosome polymorphisms in hylid frogs and the sixth reported in Anura.
Fil: Ferro, Juan Martín. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Marti, Dardo Andrea. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Bidau, Claudio Juan. Universidad Nacional de Río Negro; Argentina
Fil: Suarez, Pablo. Universidade Federal do Pará; Brasil
Fil: Nagamachi, Cleusa Y.. Universidade Federal do Pará; Brasil
Fil: Pieczarka, Júlio C.. Universidade Federal do Pará; Brasil
Fil: Baldo, Juan Diego. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Materia
Fish
Hypsiboas Albopunctatus
Polymorphism
Supernumerary Chromosome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36324
Acceder
id CONICETDig_8923017981fbe03b591e7cbc4ef0c708
oai_identifier_str oai:ri.conicet.gov.ar:11336/36324
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)Ferro, Juan MartínMarti, Dardo AndreaBidau, Claudio JuanSuarez, PabloNagamachi, Cleusa Y.Pieczarka, Júlio C.Baldo, Juan DiegoFishHypsiboas AlbopunctatusPolymorphismSupernumerary Chromosomehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Supernumerary or B chromosomes are one of the main causes for numerical chromosomal variation in higher eukaryotes. These extragenetic elements have been studied for more than a century, with the goal of trying to understand their origin, and how they survive as a polymorphism in natural populations. Hypsiboas albopunctatus is a nocturnal hylid frog distributed in the central–eastern part of South America. Previously, variation in chromosome numbers was described for a population from Rio Claro, São Paulo, Brazil, in which a single small-sized, metacentric B chromosome was present in ca. 40% of the analyzed individuals (n = 17). We herein describe the presence of B chromosomes in populations of H. albopunctatus from northeastern Argentina (Corrientes and Misiones provinces), with unusual morphological and structural characteristics. The frequency of B chromosomes varied significantly among analyzed populations. We found four diploid numbers (2N = 22, 22 + 1B, 22 + 2B, and 22 + 3B), and in a few individuals mitotic instability occurred. C banding revealed variations in the heterochromatin (DAPI+) pattern between Bs with similar morphology, indicating the existence of two new structural variants of these supernumerary elements in H. albopunctatus (B1 and B2). Nucleolar organizer regions marked positively on the eighth pair, coincident with the location of ribosomal DNA as demonstrated with fluorescent in situ hybridization, but Bs did not mark positive with these two techniques. Also, fluorescent in situ hybridization with telomeric probes showed no differences in location and intensity between Bs and autosomal chromosomes. The present communication is the first case of B chromosome polymorphisms in hylid frogs and the sixth reported in Anura.Fil: Ferro, Juan Martín. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Marti, Dardo Andrea. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Bidau, Claudio Juan. Universidad Nacional de Río Negro; ArgentinaFil: Suarez, Pablo. Universidade Federal do Pará; BrasilFil: Nagamachi, Cleusa Y.. Universidade Federal do Pará; BrasilFil: Pieczarka, Júlio C.. Universidade Federal do Pará; BrasilFil: Baldo, Juan Diego. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaHerpetologists League2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36324Ferro, Juan Martín; Marti, Dardo Andrea; Bidau, Claudio Juan; Suarez, Pablo; Nagamachi, Cleusa Y.; et al.; B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae); Herpetologists League; Herpetologica; 68; 4; 12-2012; 482-4900018-0831CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1655/HERPETOLOGICA-D-12-00020info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.1655/HERPETOLOGICA-D-12-00020info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:14Zoai:ri.conicet.gov.ar:11336/36324instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:14.81CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
title B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
spellingShingle B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
Ferro, Juan Martín
Fish
Hypsiboas Albopunctatus
Polymorphism
Supernumerary Chromosome
title_short B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
title_full B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
title_fullStr B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
title_full_unstemmed B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
title_sort B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae)
dc.creator.none.fl_str_mv Ferro, Juan Martín
Marti, Dardo Andrea
Bidau, Claudio Juan
Suarez, Pablo
Nagamachi, Cleusa Y.
Pieczarka, Júlio C.
Baldo, Juan Diego
author Ferro, Juan Martín
author_facet Ferro, Juan Martín
Marti, Dardo Andrea
Bidau, Claudio Juan
Suarez, Pablo
Nagamachi, Cleusa Y.
Pieczarka, Júlio C.
Baldo, Juan Diego
author_role author
author2 Marti, Dardo Andrea
Bidau, Claudio Juan
Suarez, Pablo
Nagamachi, Cleusa Y.
Pieczarka, Júlio C.
Baldo, Juan Diego
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Fish
Hypsiboas Albopunctatus
Polymorphism
Supernumerary Chromosome
topic Fish
Hypsiboas Albopunctatus
Polymorphism
Supernumerary Chromosome
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Supernumerary or B chromosomes are one of the main causes for numerical chromosomal variation in higher eukaryotes. These extragenetic elements have been studied for more than a century, with the goal of trying to understand their origin, and how they survive as a polymorphism in natural populations. Hypsiboas albopunctatus is a nocturnal hylid frog distributed in the central–eastern part of South America. Previously, variation in chromosome numbers was described for a population from Rio Claro, São Paulo, Brazil, in which a single small-sized, metacentric B chromosome was present in ca. 40% of the analyzed individuals (n = 17). We herein describe the presence of B chromosomes in populations of H. albopunctatus from northeastern Argentina (Corrientes and Misiones provinces), with unusual morphological and structural characteristics. The frequency of B chromosomes varied significantly among analyzed populations. We found four diploid numbers (2N = 22, 22 + 1B, 22 + 2B, and 22 + 3B), and in a few individuals mitotic instability occurred. C banding revealed variations in the heterochromatin (DAPI+) pattern between Bs with similar morphology, indicating the existence of two new structural variants of these supernumerary elements in H. albopunctatus (B1 and B2). Nucleolar organizer regions marked positively on the eighth pair, coincident with the location of ribosomal DNA as demonstrated with fluorescent in situ hybridization, but Bs did not mark positive with these two techniques. Also, fluorescent in situ hybridization with telomeric probes showed no differences in location and intensity between Bs and autosomal chromosomes. The present communication is the first case of B chromosome polymorphisms in hylid frogs and the sixth reported in Anura.
Fil: Ferro, Juan Martín. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Marti, Dardo Andrea. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Bidau, Claudio Juan. Universidad Nacional de Río Negro; Argentina
Fil: Suarez, Pablo. Universidade Federal do Pará; Brasil
Fil: Nagamachi, Cleusa Y.. Universidade Federal do Pará; Brasil
Fil: Pieczarka, Júlio C.. Universidade Federal do Pará; Brasil
Fil: Baldo, Juan Diego. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
description Supernumerary or B chromosomes are one of the main causes for numerical chromosomal variation in higher eukaryotes. These extragenetic elements have been studied for more than a century, with the goal of trying to understand their origin, and how they survive as a polymorphism in natural populations. Hypsiboas albopunctatus is a nocturnal hylid frog distributed in the central–eastern part of South America. Previously, variation in chromosome numbers was described for a population from Rio Claro, São Paulo, Brazil, in which a single small-sized, metacentric B chromosome was present in ca. 40% of the analyzed individuals (n = 17). We herein describe the presence of B chromosomes in populations of H. albopunctatus from northeastern Argentina (Corrientes and Misiones provinces), with unusual morphological and structural characteristics. The frequency of B chromosomes varied significantly among analyzed populations. We found four diploid numbers (2N = 22, 22 + 1B, 22 + 2B, and 22 + 3B), and in a few individuals mitotic instability occurred. C banding revealed variations in the heterochromatin (DAPI+) pattern between Bs with similar morphology, indicating the existence of two new structural variants of these supernumerary elements in H. albopunctatus (B1 and B2). Nucleolar organizer regions marked positively on the eighth pair, coincident with the location of ribosomal DNA as demonstrated with fluorescent in situ hybridization, but Bs did not mark positive with these two techniques. Also, fluorescent in situ hybridization with telomeric probes showed no differences in location and intensity between Bs and autosomal chromosomes. The present communication is the first case of B chromosome polymorphisms in hylid frogs and the sixth reported in Anura.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36324
Ferro, Juan Martín; Marti, Dardo Andrea; Bidau, Claudio Juan; Suarez, Pablo; Nagamachi, Cleusa Y.; et al.; B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae); Herpetologists League; Herpetologica; 68; 4; 12-2012; 482-490
0018-0831
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36324
identifier_str_mv Ferro, Juan Martín; Marti, Dardo Andrea; Bidau, Claudio Juan; Suarez, Pablo; Nagamachi, Cleusa Y.; et al.; B Chromosomes in the Tree Frog Hypsiboas albopunctatus (Anura: Hylidae); Herpetologists League; Herpetologica; 68; 4; 12-2012; 482-490
0018-0831
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1655/HERPETOLOGICA-D-12-00020
info:eu-repo/semantics/altIdentifier/url/http://www.bioone.org/doi/abs/10.1655/HERPETOLOGICA-D-12-00020
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Herpetologists League
publisher.none.fl_str_mv Herpetologists League
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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