Precipitation with poly acrylic acid as a trypsin bioseparation strategy

Autores
Porfiri, María Cecilia; Braia, Mauricio Javier; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; Romanini, Diana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Precipitation of enzymes with reversible soluble-insoluble polymers is a simple approach which can be easily scaled up. This work reports investigations aiming at verifying the existence of specific interactions and complex formation between porcine trypsin and poly acrylic acids using spectroscopy techniques. The trypsin-polymer complex was insoluble at pH lower than 5, with a stoichiometric ratio polymer mol per protein mol of 1:148. It took only a minute for the insoluble complex to form and it was redissolved modifying the pH of the medium. The enzymatic activity of trypsin was maintained even in the presence of the polymer and after precipitation poly acrylic acid presence protect the enzyme from itself degradation. The conditions of complex formation were studied using pure proteins that could be applied on porcine pancreas homogenates as an isolation strategy of trypsin.
Fil: Porfiri, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Braia, Mauricio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Farruggia, Beatriz Monica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Materia
BIOSEPARATION
POLY ACRYLIC ACID
POLYELECTROLYTE
POLYMER
PRECIPITATION
TRYPSIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95239
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95239
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Precipitation with poly acrylic acid as a trypsin bioseparation strategyPorfiri, María CeciliaBraia, Mauricio JavierFarruggia, Beatriz MonicaPicó, Guillermo AlfredoRomanini, DianaBIOSEPARATIONPOLY ACRYLIC ACIDPOLYELECTROLYTEPOLYMERPRECIPITATIONTRYPSINhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Precipitation of enzymes with reversible soluble-insoluble polymers is a simple approach which can be easily scaled up. This work reports investigations aiming at verifying the existence of specific interactions and complex formation between porcine trypsin and poly acrylic acids using spectroscopy techniques. The trypsin-polymer complex was insoluble at pH lower than 5, with a stoichiometric ratio polymer mol per protein mol of 1:148. It took only a minute for the insoluble complex to form and it was redissolved modifying the pH of the medium. The enzymatic activity of trypsin was maintained even in the presence of the polymer and after precipitation poly acrylic acid presence protect the enzyme from itself degradation. The conditions of complex formation were studied using pure proteins that could be applied on porcine pancreas homogenates as an isolation strategy of trypsin.Fil: Porfiri, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaFil: Braia, Mauricio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaFil: Farruggia, Beatriz Monica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; ArgentinaElsevier2009-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95239Porfiri, María Cecilia; Braia, Mauricio Javier; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; Romanini, Diana; Precipitation with poly acrylic acid as a trypsin bioseparation strategy; Elsevier; Process Biochemistry; 44; 9; 9-2009; 1046-10490032-95921359-5113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2009.04.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511309001172info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:55Zoai:ri.conicet.gov.ar:11336/95239instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:55.946CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Precipitation with poly acrylic acid as a trypsin bioseparation strategy
title Precipitation with poly acrylic acid as a trypsin bioseparation strategy
spellingShingle Precipitation with poly acrylic acid as a trypsin bioseparation strategy
Porfiri, María Cecilia
BIOSEPARATION
POLY ACRYLIC ACID
POLYELECTROLYTE
POLYMER
PRECIPITATION
TRYPSIN
title_short Precipitation with poly acrylic acid as a trypsin bioseparation strategy
title_full Precipitation with poly acrylic acid as a trypsin bioseparation strategy
title_fullStr Precipitation with poly acrylic acid as a trypsin bioseparation strategy
title_full_unstemmed Precipitation with poly acrylic acid as a trypsin bioseparation strategy
title_sort Precipitation with poly acrylic acid as a trypsin bioseparation strategy
dc.creator.none.fl_str_mv Porfiri, María Cecilia
Braia, Mauricio Javier
Farruggia, Beatriz Monica
Picó, Guillermo Alfredo
Romanini, Diana
author Porfiri, María Cecilia
author_facet Porfiri, María Cecilia
Braia, Mauricio Javier
Farruggia, Beatriz Monica
Picó, Guillermo Alfredo
Romanini, Diana
author_role author
author2 Braia, Mauricio Javier
Farruggia, Beatriz Monica
Picó, Guillermo Alfredo
Romanini, Diana
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOSEPARATION
POLY ACRYLIC ACID
POLYELECTROLYTE
POLYMER
PRECIPITATION
TRYPSIN
topic BIOSEPARATION
POLY ACRYLIC ACID
POLYELECTROLYTE
POLYMER
PRECIPITATION
TRYPSIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Precipitation of enzymes with reversible soluble-insoluble polymers is a simple approach which can be easily scaled up. This work reports investigations aiming at verifying the existence of specific interactions and complex formation between porcine trypsin and poly acrylic acids using spectroscopy techniques. The trypsin-polymer complex was insoluble at pH lower than 5, with a stoichiometric ratio polymer mol per protein mol of 1:148. It took only a minute for the insoluble complex to form and it was redissolved modifying the pH of the medium. The enzymatic activity of trypsin was maintained even in the presence of the polymer and after precipitation poly acrylic acid presence protect the enzyme from itself degradation. The conditions of complex formation were studied using pure proteins that could be applied on porcine pancreas homogenates as an isolation strategy of trypsin.
Fil: Porfiri, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Braia, Mauricio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
Fil: Farruggia, Beatriz Monica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Picó, Guillermo Alfredo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Romanini, Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química y Física. Área Fisicoquímica; Argentina
description Precipitation of enzymes with reversible soluble-insoluble polymers is a simple approach which can be easily scaled up. This work reports investigations aiming at verifying the existence of specific interactions and complex formation between porcine trypsin and poly acrylic acids using spectroscopy techniques. The trypsin-polymer complex was insoluble at pH lower than 5, with a stoichiometric ratio polymer mol per protein mol of 1:148. It took only a minute for the insoluble complex to form and it was redissolved modifying the pH of the medium. The enzymatic activity of trypsin was maintained even in the presence of the polymer and after precipitation poly acrylic acid presence protect the enzyme from itself degradation. The conditions of complex formation were studied using pure proteins that could be applied on porcine pancreas homogenates as an isolation strategy of trypsin.
publishDate 2009
dc.date.none.fl_str_mv 2009-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95239
Porfiri, María Cecilia; Braia, Mauricio Javier; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; Romanini, Diana; Precipitation with poly acrylic acid as a trypsin bioseparation strategy; Elsevier; Process Biochemistry; 44; 9; 9-2009; 1046-1049
0032-9592
1359-5113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95239
identifier_str_mv Porfiri, María Cecilia; Braia, Mauricio Javier; Farruggia, Beatriz Monica; Picó, Guillermo Alfredo; Romanini, Diana; Precipitation with poly acrylic acid as a trypsin bioseparation strategy; Elsevier; Process Biochemistry; 44; 9; 9-2009; 1046-1049
0032-9592
1359-5113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.procbio.2009.04.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1359511309001172
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269002272866304
score 13.13397

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