Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis

Autores
Aguilera, Andrea Carolina; Carvelli, Flavia Lorena; Boschin, Veronica; Mohamed, Fabian Heber; Zyla, Leila Ester; Sosa Escudero, Miguel Angel
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the most striking features of the mammalian epididymis is the secretion of lysosomal enzymes (LE). These LE may play a role in sperm maturation. In the present study we investigated the activity and distribution of four LE (b-galactosidase (b-Gal), N-acetyl-b-D-glucosaminidase (b-NAG), a-mannosidase (a-Man) and b-glucuronidase) in bull epididymis at two different ages (6 months and 4 years) to determine whether these enzymes vary with sexual 5 maturity. In young, sexually immature (SI) bulls we found high LE activity in the epididymal tissue that accounts for a developed and active lysosomal apparatus. In contrast, low LE activity was measured in sexually mature (SM) bulls, and b-NAG and b-Gal were mostly secreted into the lumen. We also attempted to correlate LE distribution with the expression and functionality of mannose-6-phosphate receptors (MPRs), which are thought to be involved in proper delivery of LE to lysosomes. The cation-dependent MPR was highly expressed in SI bulls, with expression decreasing during adulthood, 10 whereas the expression of the cation-independent MPR was higher in SM than SI bulls. In addition, the four enzymes recovered from the epididymal lumen interact with both MPRs at each age. We conclude that the activity and distribution of LE in bull epididymis varies with sexual maturity and that the distribution is regulated differently by the two types of MPR. These findings could provide some molecular basis for male infertility.
Fil: Aguilera, Andrea Carolina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Carvelli, Flavia Lorena. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Boschin, Veronica. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis; Argentina
Fil: Zyla, Leila Ester. Facultad de Ciencias Médicas. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Sosa Escudero, Miguel Angel. Facultad de Ciencias Médicas. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Epididymis
Lysosomal Enzymes
Mannose-6-Phosphate Receptors
Protein Secretion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41889
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41889
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymisAguilera, Andrea CarolinaCarvelli, Flavia LorenaBoschin, VeronicaMohamed, Fabian HeberZyla, Leila EsterSosa Escudero, Miguel AngelEpididymisLysosomal EnzymesMannose-6-Phosphate ReceptorsProtein Secretionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1One of the most striking features of the mammalian epididymis is the secretion of lysosomal enzymes (LE). These LE may play a role in sperm maturation. In the present study we investigated the activity and distribution of four LE (b-galactosidase (b-Gal), N-acetyl-b-D-glucosaminidase (b-NAG), a-mannosidase (a-Man) and b-glucuronidase) in bull epididymis at two different ages (6 months and 4 years) to determine whether these enzymes vary with sexual 5 maturity. In young, sexually immature (SI) bulls we found high LE activity in the epididymal tissue that accounts for a developed and active lysosomal apparatus. In contrast, low LE activity was measured in sexually mature (SM) bulls, and b-NAG and b-Gal were mostly secreted into the lumen. We also attempted to correlate LE distribution with the expression and functionality of mannose-6-phosphate receptors (MPRs), which are thought to be involved in proper delivery of LE to lysosomes. The cation-dependent MPR was highly expressed in SI bulls, with expression decreasing during adulthood, 10 whereas the expression of the cation-independent MPR was higher in SM than SI bulls. In addition, the four enzymes recovered from the epididymal lumen interact with both MPRs at each age. We conclude that the activity and distribution of LE in bull epididymis varies with sexual maturity and that the distribution is regulated differently by the two types of MPR. These findings could provide some molecular basis for male infertility.Fil: Aguilera, Andrea Carolina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Carvelli, Flavia Lorena. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Boschin, Veronica. Universidad del Aconcagua. Facultad de Ciencias Médicas; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis; ArgentinaFil: Zyla, Leila Ester. Facultad de Ciencias Médicas. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Sosa Escudero, Miguel Angel. Facultad de Ciencias Médicas. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaCsiro Publishing2015-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41889Aguilera, Andrea Carolina; Carvelli, Flavia Lorena; Boschin, Veronica; Mohamed, Fabian Heber; Zyla, Leila Ester; et al.; Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis; Csiro Publishing; Reproduction Fertility and Development; 28; 10; 4-2015; 1608-16171031-3613CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1071/RD14380info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD14380info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:19Zoai:ri.conicet.gov.ar:11336/41889instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:20.014CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
title Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
spellingShingle Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
Aguilera, Andrea Carolina
Epididymis
Lysosomal Enzymes
Mannose-6-Phosphate Receptors
Protein Secretion
title_short Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
title_full Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
title_fullStr Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
title_full_unstemmed Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
title_sort Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis
dc.creator.none.fl_str_mv Aguilera, Andrea Carolina
Carvelli, Flavia Lorena
Boschin, Veronica
Mohamed, Fabian Heber
Zyla, Leila Ester
Sosa Escudero, Miguel Angel
author Aguilera, Andrea Carolina
author_facet Aguilera, Andrea Carolina
Carvelli, Flavia Lorena
Boschin, Veronica
Mohamed, Fabian Heber
Zyla, Leila Ester
Sosa Escudero, Miguel Angel
author_role author
author2 Carvelli, Flavia Lorena
Boschin, Veronica
Mohamed, Fabian Heber
Zyla, Leila Ester
Sosa Escudero, Miguel Angel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Epididymis
Lysosomal Enzymes
Mannose-6-Phosphate Receptors
Protein Secretion
topic Epididymis
Lysosomal Enzymes
Mannose-6-Phosphate Receptors
Protein Secretion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv One of the most striking features of the mammalian epididymis is the secretion of lysosomal enzymes (LE). These LE may play a role in sperm maturation. In the present study we investigated the activity and distribution of four LE (b-galactosidase (b-Gal), N-acetyl-b-D-glucosaminidase (b-NAG), a-mannosidase (a-Man) and b-glucuronidase) in bull epididymis at two different ages (6 months and 4 years) to determine whether these enzymes vary with sexual 5 maturity. In young, sexually immature (SI) bulls we found high LE activity in the epididymal tissue that accounts for a developed and active lysosomal apparatus. In contrast, low LE activity was measured in sexually mature (SM) bulls, and b-NAG and b-Gal were mostly secreted into the lumen. We also attempted to correlate LE distribution with the expression and functionality of mannose-6-phosphate receptors (MPRs), which are thought to be involved in proper delivery of LE to lysosomes. The cation-dependent MPR was highly expressed in SI bulls, with expression decreasing during adulthood, 10 whereas the expression of the cation-independent MPR was higher in SM than SI bulls. In addition, the four enzymes recovered from the epididymal lumen interact with both MPRs at each age. We conclude that the activity and distribution of LE in bull epididymis varies with sexual maturity and that the distribution is regulated differently by the two types of MPR. These findings could provide some molecular basis for male infertility.
Fil: Aguilera, Andrea Carolina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Carvelli, Flavia Lorena. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Boschin, Veronica. Universidad del Aconcagua. Facultad de Ciencias Médicas; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis; Argentina
Fil: Zyla, Leila Ester. Facultad de Ciencias Médicas. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Sosa Escudero, Miguel Angel. Facultad de Ciencias Médicas. Universidad Nacional de Cuyo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description One of the most striking features of the mammalian epididymis is the secretion of lysosomal enzymes (LE). These LE may play a role in sperm maturation. In the present study we investigated the activity and distribution of four LE (b-galactosidase (b-Gal), N-acetyl-b-D-glucosaminidase (b-NAG), a-mannosidase (a-Man) and b-glucuronidase) in bull epididymis at two different ages (6 months and 4 years) to determine whether these enzymes vary with sexual 5 maturity. In young, sexually immature (SI) bulls we found high LE activity in the epididymal tissue that accounts for a developed and active lysosomal apparatus. In contrast, low LE activity was measured in sexually mature (SM) bulls, and b-NAG and b-Gal were mostly secreted into the lumen. We also attempted to correlate LE distribution with the expression and functionality of mannose-6-phosphate receptors (MPRs), which are thought to be involved in proper delivery of LE to lysosomes. The cation-dependent MPR was highly expressed in SI bulls, with expression decreasing during adulthood, 10 whereas the expression of the cation-independent MPR was higher in SM than SI bulls. In addition, the four enzymes recovered from the epididymal lumen interact with both MPRs at each age. We conclude that the activity and distribution of LE in bull epididymis varies with sexual maturity and that the distribution is regulated differently by the two types of MPR. These findings could provide some molecular basis for male infertility.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41889
Aguilera, Andrea Carolina; Carvelli, Flavia Lorena; Boschin, Veronica; Mohamed, Fabian Heber; Zyla, Leila Ester; et al.; Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis; Csiro Publishing; Reproduction Fertility and Development; 28; 10; 4-2015; 1608-1617
1031-3613
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41889
identifier_str_mv Aguilera, Andrea Carolina; Carvelli, Flavia Lorena; Boschin, Veronica; Mohamed, Fabian Heber; Zyla, Leila Ester; et al.; Changes in lysosomal enzymes and mannose-6-phosphate receptors related to sexual maturation in bull epididymis; Csiro Publishing; Reproduction Fertility and Development; 28; 10; 4-2015; 1608-1617
1031-3613
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1071/RD14380
info:eu-repo/semantics/altIdentifier/url/http://www.publish.csiro.au/rd/RD14380
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Csiro Publishing
publisher.none.fl_str_mv Csiro Publishing
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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