Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus

Autores
Benevenuto, Juliana; Longatto, Daniel P.; Reis, Gislaine V.; Mielnichuk, Natalia; Palhares, Alessandra C.; Carvalho, Giselle; Saito, Suzane; Quecine, Maria C.; Sanguino, Alvaro; Vieira, Maria Lucia C.; Camargo, Luis Eduardo A.; Creste, Silvana; Monteiro-Vitorello, Claudia B.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sporisorium scitamineum is the fungus that causes sugarcane smut disease. Despite of the importance of sugarcane for Brazilian agribusiness and the persistence of the pathogen in most cropping areas, genetic variation studies are still missing for Brazilian isolates. In this study, sets of isolates were analyzed using two molecular markers (AFLP and telRFLP) and ITS sequencing. Twenty-two whips were collected from symptomatic plants in cultivated sugarcane fields of Brazil. A total of 41 haploid strains of compatible mating types were selected from individual teliospores and used for molecular genetic analyses. telRFLP and ITS analyses were expanded to six Argentine isolates, where the sugarcane smut was first recorded in America. Genetic relationship among strains suggests the human-mediated dispersal of S. scitamineum within the Brazilian territory and between the two neighboring countries. Two genetically distinct groups were defined by the combined analysis of AFLP and telRFLP. The opposite mating-type strains derived from single teliospores were clustered together into these main groups, but had not always identical haplotypes. telRFLP markers analyzed over two generations of selfing and controlled outcrossing confirmed the potential for emergence of new variants and occurrence of recombination, which are relevant events for evolution of virulence and environmental adaptation.
Fil: Benevenuto, Juliana. Universidade de Sao Paulo; Brasil
Fil: Longatto, Daniel P.. Universidade de Sao Paulo; Brasil
Fil: Reis, Gislaine V.. Universidade de Sao Paulo; Brasil
Fil: Mielnichuk, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palhares, Alessandra C.. Universidade de Sao Paulo; Brasil
Fil: Carvalho, Giselle. Universidade de Sao Paulo; Brasil
Fil: Saito, Suzane. Universidade de Sao Paulo; Brasil
Fil: Quecine, Maria C.. Universidade de Sao Paulo; Brasil
Fil: Sanguino, Alvaro. Agencia Paulista de Tecnologia Dos Agronegocios; Brasil
Fil: Vieira, Maria Lucia C.. Universidade de Sao Paulo; Brasil
Fil: Camargo, Luis Eduardo A.. Universidade de Sao Paulo; Brasil
Fil: Creste, Silvana. Agencia Paulista de Tecnologia Dos Agronegocios; Brasil
Fil: Monteiro-Vitorello, Claudia B.. Universidade de Sao Paulo; Brasil
Materia
Argentina
Brazil
Mating-Type
Molecular Markers
Sporisorium Scitamineum
Telomere
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40320
Acceder
id CONICETDig_8819d94383017dc1a32e6d569ee51628
oai_identifier_str oai:ri.conicet.gov.ar:11336/40320
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungusBenevenuto, JulianaLongatto, Daniel P.Reis, Gislaine V.Mielnichuk, NataliaPalhares, Alessandra C.Carvalho, GiselleSaito, SuzaneQuecine, Maria C.Sanguino, AlvaroVieira, Maria Lucia C.Camargo, Luis Eduardo A.Creste, SilvanaMonteiro-Vitorello, Claudia B.ArgentinaBrazilMating-TypeMolecular MarkersSporisorium ScitamineumTelomerehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sporisorium scitamineum is the fungus that causes sugarcane smut disease. Despite of the importance of sugarcane for Brazilian agribusiness and the persistence of the pathogen in most cropping areas, genetic variation studies are still missing for Brazilian isolates. In this study, sets of isolates were analyzed using two molecular markers (AFLP and telRFLP) and ITS sequencing. Twenty-two whips were collected from symptomatic plants in cultivated sugarcane fields of Brazil. A total of 41 haploid strains of compatible mating types were selected from individual teliospores and used for molecular genetic analyses. telRFLP and ITS analyses were expanded to six Argentine isolates, where the sugarcane smut was first recorded in America. Genetic relationship among strains suggests the human-mediated dispersal of S. scitamineum within the Brazilian territory and between the two neighboring countries. Two genetically distinct groups were defined by the combined analysis of AFLP and telRFLP. The opposite mating-type strains derived from single teliospores were clustered together into these main groups, but had not always identical haplotypes. telRFLP markers analyzed over two generations of selfing and controlled outcrossing confirmed the potential for emergence of new variants and occurrence of recombination, which are relevant events for evolution of virulence and environmental adaptation.Fil: Benevenuto, Juliana. Universidade de Sao Paulo; BrasilFil: Longatto, Daniel P.. Universidade de Sao Paulo; BrasilFil: Reis, Gislaine V.. Universidade de Sao Paulo; BrasilFil: Mielnichuk, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Palhares, Alessandra C.. Universidade de Sao Paulo; BrasilFil: Carvalho, Giselle. Universidade de Sao Paulo; BrasilFil: Saito, Suzane. Universidade de Sao Paulo; BrasilFil: Quecine, Maria C.. Universidade de Sao Paulo; BrasilFil: Sanguino, Alvaro. Agencia Paulista de Tecnologia Dos Agronegocios; BrasilFil: Vieira, Maria Lucia C.. Universidade de Sao Paulo; BrasilFil: Camargo, Luis Eduardo A.. Universidade de Sao Paulo; BrasilFil: Creste, Silvana. Agencia Paulista de Tecnologia Dos Agronegocios; BrasilFil: Monteiro-Vitorello, Claudia B.. Universidade de Sao Paulo; BrasilOxford University Press2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40320Benevenuto, Juliana; Longatto, Daniel P.; Reis, Gislaine V.; Mielnichuk, Natalia; Palhares, Alessandra C.; et al.; Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus; Oxford University Press; FEMS Microbiology Letters; 363; 24; 12-20160378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnw277info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/363/24/fnw277/2660843info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-01-08T12:56:49Zoai:ri.conicet.gov.ar:11336/40320instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-01-08 12:56:49.883CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
title Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
spellingShingle Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
Benevenuto, Juliana
Argentina
Brazil
Mating-Type
Molecular Markers
Sporisorium Scitamineum
Telomere
title_short Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
title_full Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
title_fullStr Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
title_full_unstemmed Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
title_sort Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus
dc.creator.none.fl_str_mv Benevenuto, Juliana
Longatto, Daniel P.
Reis, Gislaine V.
Mielnichuk, Natalia
Palhares, Alessandra C.
Carvalho, Giselle
Saito, Suzane
Quecine, Maria C.
Sanguino, Alvaro
Vieira, Maria Lucia C.
Camargo, Luis Eduardo A.
Creste, Silvana
Monteiro-Vitorello, Claudia B.
author Benevenuto, Juliana
author_facet Benevenuto, Juliana
Longatto, Daniel P.
Reis, Gislaine V.
Mielnichuk, Natalia
Palhares, Alessandra C.
Carvalho, Giselle
Saito, Suzane
Quecine, Maria C.
Sanguino, Alvaro
Vieira, Maria Lucia C.
Camargo, Luis Eduardo A.
Creste, Silvana
Monteiro-Vitorello, Claudia B.
author_role author
author2 Longatto, Daniel P.
Reis, Gislaine V.
Mielnichuk, Natalia
Palhares, Alessandra C.
Carvalho, Giselle
Saito, Suzane
Quecine, Maria C.
Sanguino, Alvaro
Vieira, Maria Lucia C.
Camargo, Luis Eduardo A.
Creste, Silvana
Monteiro-Vitorello, Claudia B.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Argentina
Brazil
Mating-Type
Molecular Markers
Sporisorium Scitamineum
Telomere
topic Argentina
Brazil
Mating-Type
Molecular Markers
Sporisorium Scitamineum
Telomere
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sporisorium scitamineum is the fungus that causes sugarcane smut disease. Despite of the importance of sugarcane for Brazilian agribusiness and the persistence of the pathogen in most cropping areas, genetic variation studies are still missing for Brazilian isolates. In this study, sets of isolates were analyzed using two molecular markers (AFLP and telRFLP) and ITS sequencing. Twenty-two whips were collected from symptomatic plants in cultivated sugarcane fields of Brazil. A total of 41 haploid strains of compatible mating types were selected from individual teliospores and used for molecular genetic analyses. telRFLP and ITS analyses were expanded to six Argentine isolates, where the sugarcane smut was first recorded in America. Genetic relationship among strains suggests the human-mediated dispersal of S. scitamineum within the Brazilian territory and between the two neighboring countries. Two genetically distinct groups were defined by the combined analysis of AFLP and telRFLP. The opposite mating-type strains derived from single teliospores were clustered together into these main groups, but had not always identical haplotypes. telRFLP markers analyzed over two generations of selfing and controlled outcrossing confirmed the potential for emergence of new variants and occurrence of recombination, which are relevant events for evolution of virulence and environmental adaptation.
Fil: Benevenuto, Juliana. Universidade de Sao Paulo; Brasil
Fil: Longatto, Daniel P.. Universidade de Sao Paulo; Brasil
Fil: Reis, Gislaine V.. Universidade de Sao Paulo; Brasil
Fil: Mielnichuk, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Palhares, Alessandra C.. Universidade de Sao Paulo; Brasil
Fil: Carvalho, Giselle. Universidade de Sao Paulo; Brasil
Fil: Saito, Suzane. Universidade de Sao Paulo; Brasil
Fil: Quecine, Maria C.. Universidade de Sao Paulo; Brasil
Fil: Sanguino, Alvaro. Agencia Paulista de Tecnologia Dos Agronegocios; Brasil
Fil: Vieira, Maria Lucia C.. Universidade de Sao Paulo; Brasil
Fil: Camargo, Luis Eduardo A.. Universidade de Sao Paulo; Brasil
Fil: Creste, Silvana. Agencia Paulista de Tecnologia Dos Agronegocios; Brasil
Fil: Monteiro-Vitorello, Claudia B.. Universidade de Sao Paulo; Brasil
description Sporisorium scitamineum is the fungus that causes sugarcane smut disease. Despite of the importance of sugarcane for Brazilian agribusiness and the persistence of the pathogen in most cropping areas, genetic variation studies are still missing for Brazilian isolates. In this study, sets of isolates were analyzed using two molecular markers (AFLP and telRFLP) and ITS sequencing. Twenty-two whips were collected from symptomatic plants in cultivated sugarcane fields of Brazil. A total of 41 haploid strains of compatible mating types were selected from individual teliospores and used for molecular genetic analyses. telRFLP and ITS analyses were expanded to six Argentine isolates, where the sugarcane smut was first recorded in America. Genetic relationship among strains suggests the human-mediated dispersal of S. scitamineum within the Brazilian territory and between the two neighboring countries. Two genetically distinct groups were defined by the combined analysis of AFLP and telRFLP. The opposite mating-type strains derived from single teliospores were clustered together into these main groups, but had not always identical haplotypes. telRFLP markers analyzed over two generations of selfing and controlled outcrossing confirmed the potential for emergence of new variants and occurrence of recombination, which are relevant events for evolution of virulence and environmental adaptation.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40320
Benevenuto, Juliana; Longatto, Daniel P.; Reis, Gislaine V.; Mielnichuk, Natalia; Palhares, Alessandra C.; et al.; Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus; Oxford University Press; FEMS Microbiology Letters; 363; 24; 12-2016
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40320
identifier_str_mv Benevenuto, Juliana; Longatto, Daniel P.; Reis, Gislaine V.; Mielnichuk, Natalia; Palhares, Alessandra C.; et al.; Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus; Oxford University Press; FEMS Microbiology Letters; 363; 24; 12-2016
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/femsle/fnw277
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/363/24/fnw277/2660843
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.25844

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