Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation

Autores
Hernández Silva, Gabriela; López Torres, Aideé S.; Maldonado Rosas, Israel; Mata Martínez, Esperanza; Larrea, Fernando; Torres Flores, Víctor; Treviño, Claudia L.; Chirinos, Mayel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories. Materials and Methods: To compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions. Results: Semen samples processed in parallel by these two procedures resulted in sperm preparations with significant differences in redox state, spontaneous intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction responsivity to calcium ionophore. Such differences showed time-dependent specific patterns for spontaneous intracellular calcium oscillations, hyperactivation and protein tyrosine phosphorylation. Sperm retrieved by density gradient centrifugation showed more hyperactivation and tyrosine phosphorylation than swim-up sperm, suggesting a higher degree of capacitation. Conclusions: Our results account for functional differences observed in spermatozoa processed with these two methods and therefore may contribute to a better interpretation of outcomes obtained in different laboratories as well as to improve experimental designs aimed to study sperm physiology and fertility potential.
Fil: Hernández Silva, Gabriela. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: López Torres, Aideé S.. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Maldonado Rosas, Israel. Centro de Innovación Tecnológica y Medicina Reproductiva; México
Fil: Mata Martínez, Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Torres Flores, Víctor. Universidad Nacional Autónoma de México; México
Fil: Treviño, Claudia L.. Universidad Nacional Autónoma de México; México
Fil: Chirinos, Mayel. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Materia
FERTILITY
SPERM CAPACITATION
SPERM RETRIEVAL
SPERMATOZOA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141329
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/141329
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugationHernández Silva, GabrielaLópez Torres, Aideé S.Maldonado Rosas, IsraelMata Martínez, EsperanzaLarrea, FernandoTorres Flores, VíctorTreviño, Claudia L.Chirinos, MayelFERTILITYSPERM CAPACITATIONSPERM RETRIEVALSPERMATOZOAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories. Materials and Methods: To compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions. Results: Semen samples processed in parallel by these two procedures resulted in sperm preparations with significant differences in redox state, spontaneous intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction responsivity to calcium ionophore. Such differences showed time-dependent specific patterns for spontaneous intracellular calcium oscillations, hyperactivation and protein tyrosine phosphorylation. Sperm retrieved by density gradient centrifugation showed more hyperactivation and tyrosine phosphorylation than swim-up sperm, suggesting a higher degree of capacitation. Conclusions: Our results account for functional differences observed in spermatozoa processed with these two methods and therefore may contribute to a better interpretation of outcomes obtained in different laboratories as well as to improve experimental designs aimed to study sperm physiology and fertility potential.Fil: Hernández Silva, Gabriela. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; MéxicoFil: López Torres, Aideé S.. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; MéxicoFil: Maldonado Rosas, Israel. Centro de Innovación Tecnológica y Medicina Reproductiva; MéxicoFil: Mata Martínez, Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; MéxicoFil: Torres Flores, Víctor. Universidad Nacional Autónoma de México; MéxicoFil: Treviño, Claudia L.. Universidad Nacional Autónoma de México; MéxicoFil: Chirinos, Mayel. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; MéxicoKorean Society for Sexual Medicine and Andrology2020-11-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141329Hernández Silva, Gabriela; López Torres, Aideé S.; Maldonado Rosas, Israel; Mata Martínez, Esperanza; Larrea, Fernando; et al.; Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation; Korean Society for Sexual Medicine and Andrology; World Journal of Men's Health; 38; 19-11-2020; 1-102287-42082287-4690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.5534/wjmh.200115info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:54Zoai:ri.conicet.gov.ar:11336/141329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:54.873CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
title Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
spellingShingle Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
Hernández Silva, Gabriela
FERTILITY
SPERM CAPACITATION
SPERM RETRIEVAL
SPERMATOZOA
title_short Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
title_full Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
title_fullStr Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
title_full_unstemmed Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
title_sort Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation
dc.creator.none.fl_str_mv Hernández Silva, Gabriela
López Torres, Aideé S.
Maldonado Rosas, Israel
Mata Martínez, Esperanza
Larrea, Fernando
Torres Flores, Víctor
Treviño, Claudia L.
Chirinos, Mayel
author Hernández Silva, Gabriela
author_facet Hernández Silva, Gabriela
López Torres, Aideé S.
Maldonado Rosas, Israel
Mata Martínez, Esperanza
Larrea, Fernando
Torres Flores, Víctor
Treviño, Claudia L.
Chirinos, Mayel
author_role author
author2 López Torres, Aideé S.
Maldonado Rosas, Israel
Mata Martínez, Esperanza
Larrea, Fernando
Torres Flores, Víctor
Treviño, Claudia L.
Chirinos, Mayel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FERTILITY
SPERM CAPACITATION
SPERM RETRIEVAL
SPERMATOZOA
topic FERTILITY
SPERM CAPACITATION
SPERM RETRIEVAL
SPERMATOZOA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories. Materials and Methods: To compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions. Results: Semen samples processed in parallel by these two procedures resulted in sperm preparations with significant differences in redox state, spontaneous intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction responsivity to calcium ionophore. Such differences showed time-dependent specific patterns for spontaneous intracellular calcium oscillations, hyperactivation and protein tyrosine phosphorylation. Sperm retrieved by density gradient centrifugation showed more hyperactivation and tyrosine phosphorylation than swim-up sperm, suggesting a higher degree of capacitation. Conclusions: Our results account for functional differences observed in spermatozoa processed with these two methods and therefore may contribute to a better interpretation of outcomes obtained in different laboratories as well as to improve experimental designs aimed to study sperm physiology and fertility potential.
Fil: Hernández Silva, Gabriela. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: López Torres, Aideé S.. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Maldonado Rosas, Israel. Centro de Innovación Tecnológica y Medicina Reproductiva; México
Fil: Mata Martínez, Esperanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Larrea, Fernando. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
Fil: Torres Flores, Víctor. Universidad Nacional Autónoma de México; México
Fil: Treviño, Claudia L.. Universidad Nacional Autónoma de México; México
Fil: Chirinos, Mayel. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán; México
description Purpose: Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories. Materials and Methods: To compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions. Results: Semen samples processed in parallel by these two procedures resulted in sperm preparations with significant differences in redox state, spontaneous intracellular calcium oscillations, hyperactivation, protein tyrosine phosphorylation, and acrosome reaction responsivity to calcium ionophore. Such differences showed time-dependent specific patterns for spontaneous intracellular calcium oscillations, hyperactivation and protein tyrosine phosphorylation. Sperm retrieved by density gradient centrifugation showed more hyperactivation and tyrosine phosphorylation than swim-up sperm, suggesting a higher degree of capacitation. Conclusions: Our results account for functional differences observed in spermatozoa processed with these two methods and therefore may contribute to a better interpretation of outcomes obtained in different laboratories as well as to improve experimental designs aimed to study sperm physiology and fertility potential.
publishDate 2020
dc.date.none.fl_str_mv 2020-11-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141329
Hernández Silva, Gabriela; López Torres, Aideé S.; Maldonado Rosas, Israel; Mata Martínez, Esperanza; Larrea, Fernando; et al.; Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation; Korean Society for Sexual Medicine and Andrology; World Journal of Men's Health; 38; 19-11-2020; 1-10
2287-4208
2287-4690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141329
identifier_str_mv Hernández Silva, Gabriela; López Torres, Aideé S.; Maldonado Rosas, Israel; Mata Martínez, Esperanza; Larrea, Fernando; et al.; Effects of semen processing on sperm function: Differences between swim-up and density gradient centrifugation; Korean Society for Sexual Medicine and Andrology; World Journal of Men's Health; 38; 19-11-2020; 1-10
2287-4208
2287-4690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.5534/wjmh.200115
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Korean Society for Sexual Medicine and Andrology
publisher.none.fl_str_mv Korean Society for Sexual Medicine and Andrology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270098695389184
score 13.13397

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