Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus

Autores
Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The occurrence, characteristics and response to environmental salinity and dopamine of alkaline phosphatase (AP) activity were studied in chela muscle of the euryhaline crab Cyrtograpsus angulatus from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited a high AP activity with a Michaelis –Menten kinetic (Km=1.21 mM). AP activity was strongly inhibited by EDTA (I50=2.26 mM). AP activity appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10x) AP activity was lower than in 35x salinity. Upon an abrupt change to reduced salinity a short-term decrease of AP activity occurred, concomitant with the transition to hyperregulation. Furthermore, AP activity appeared to be under hormonal control since it was inhibited ‘‘in vivo’’ by 10 4 M dopamine. The response to both environmental salinity and dopamine suggests that AP activity could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation of C. angulatus. The possible functional relationship of AP activity with Na+ /K+ ATPase in muscle is discussed.
Fil: Pinoni, Silvina Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
Materia
ALKALINE PHOSPHATASE
CRABS
CYRTOGRAPSUS ANGULATUS
DOPAMINE
MUSCLE ENZYMES
OSMO-IONORREGULATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160082
Acceder
id CONICETDig_86e5beb52b39aa67fcce1003b8533963
oai_identifier_str oai:ri.conicet.gov.ar:11336/160082
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatusPinoni, Silvina AndreaLopez Mañanes, Alejandra AntoniaALKALINE PHOSPHATASECRABSCYRTOGRAPSUS ANGULATUSDOPAMINEMUSCLE ENZYMESOSMO-IONORREGULATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The occurrence, characteristics and response to environmental salinity and dopamine of alkaline phosphatase (AP) activity were studied in chela muscle of the euryhaline crab Cyrtograpsus angulatus from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited a high AP activity with a Michaelis –Menten kinetic (Km=1.21 mM). AP activity was strongly inhibited by EDTA (I50=2.26 mM). AP activity appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10x) AP activity was lower than in 35x salinity. Upon an abrupt change to reduced salinity a short-term decrease of AP activity occurred, concomitant with the transition to hyperregulation. Furthermore, AP activity appeared to be under hormonal control since it was inhibited ‘‘in vivo’’ by 10 4 M dopamine. The response to both environmental salinity and dopamine suggests that AP activity could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation of C. angulatus. The possible functional relationship of AP activity with Na+ /K+ ATPase in muscle is discussed.Fil: Pinoni, Silvina Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; ArgentinaElsevier Science2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160082Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 307; 8-2004; 35-460022-0981CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0022098104000863info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2004.01.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:38Zoai:ri.conicet.gov.ar:11336/160082instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:39.097CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
title Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
spellingShingle Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
Pinoni, Silvina Andrea
ALKALINE PHOSPHATASE
CRABS
CYRTOGRAPSUS ANGULATUS
DOPAMINE
MUSCLE ENZYMES
OSMO-IONORREGULATION
title_short Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
title_full Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
title_fullStr Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
title_full_unstemmed Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
title_sort Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus
dc.creator.none.fl_str_mv Pinoni, Silvina Andrea
Lopez Mañanes, Alejandra Antonia
author Pinoni, Silvina Andrea
author_facet Pinoni, Silvina Andrea
Lopez Mañanes, Alejandra Antonia
author_role author
author2 Lopez Mañanes, Alejandra Antonia
author2_role author
dc.subject.none.fl_str_mv ALKALINE PHOSPHATASE
CRABS
CYRTOGRAPSUS ANGULATUS
DOPAMINE
MUSCLE ENZYMES
OSMO-IONORREGULATION
topic ALKALINE PHOSPHATASE
CRABS
CYRTOGRAPSUS ANGULATUS
DOPAMINE
MUSCLE ENZYMES
OSMO-IONORREGULATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The occurrence, characteristics and response to environmental salinity and dopamine of alkaline phosphatase (AP) activity were studied in chela muscle of the euryhaline crab Cyrtograpsus angulatus from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited a high AP activity with a Michaelis –Menten kinetic (Km=1.21 mM). AP activity was strongly inhibited by EDTA (I50=2.26 mM). AP activity appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10x) AP activity was lower than in 35x salinity. Upon an abrupt change to reduced salinity a short-term decrease of AP activity occurred, concomitant with the transition to hyperregulation. Furthermore, AP activity appeared to be under hormonal control since it was inhibited ‘‘in vivo’’ by 10 4 M dopamine. The response to both environmental salinity and dopamine suggests that AP activity could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation of C. angulatus. The possible functional relationship of AP activity with Na+ /K+ ATPase in muscle is discussed.
Fil: Pinoni, Silvina Andrea. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Lopez Mañanes, Alejandra Antonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología; Argentina
description The occurrence, characteristics and response to environmental salinity and dopamine of alkaline phosphatase (AP) activity were studied in chela muscle of the euryhaline crab Cyrtograpsus angulatus from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina). Chela muscle exhibited a high AP activity with a Michaelis –Menten kinetic (Km=1.21 mM). AP activity was strongly inhibited by EDTA (I50=2.26 mM). AP activity appeared to be sensitive to environmental salinity. In crabs acclimated to low salinity (10x) AP activity was lower than in 35x salinity. Upon an abrupt change to reduced salinity a short-term decrease of AP activity occurred, concomitant with the transition to hyperregulation. Furthermore, AP activity appeared to be under hormonal control since it was inhibited ‘‘in vivo’’ by 10 4 M dopamine. The response to both environmental salinity and dopamine suggests that AP activity could be a component of muscle regulatory mechanisms at the biochemical level secondary to hyperregulation of C. angulatus. The possible functional relationship of AP activity with Na+ /K+ ATPase in muscle is discussed.
publishDate 2004
dc.date.none.fl_str_mv 2004-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160082
Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 307; 8-2004; 35-46
0022-0981
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160082
identifier_str_mv Pinoni, Silvina Andrea; Lopez Mañanes, Alejandra Antonia; Alkaline phosphatase activity sensitive to environmental salinity and dopamine in muscle of the euryhaline crab Cyrtograpsus angulatus; Elsevier Science; Journal of Experimental Marine Biology and Ecology; 307; 8-2004; 35-46
0022-0981
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0022098104000863
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jembe.2004.01.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269709372751872
score 13.13397

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