Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentin...

Autores
Hernández Orts, Jesús Servando; Aznar, Francisco J.; Blasco Costa, Isabel; Garcia, Nestor Anibal; Víllora Montero, María; Crespo, Enrique Alberto; Raga, Juan A.; Montero. Francisco E.
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Third-stage larvae of the Pseudoterranova decipiens species complex (also known as sealworms) have been reported in at least 40 marine fish species belonging to 21 families and 10 orders along the South American coast. Sealworms are a cause for concern because they can infect humans who consume raw or undercooked fish. However, despite their economic and zoonotic importance, morphological and molecular characterization of species of Pseudoterranova in South America is still scarce. Methods: A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. Results: A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver. Conclusions: We provided the first molecular identification, morphological description and microhabitat characterization of sealworm larvae from the Argentinean Patagonian coast. We also reported the infection levels of sealworms on 20 fish species in order to elucidate the life cycle of these nematodes in this area.
Fil: Hernández Orts, Jesús Servando. Academy of Sciences of the Czech Republic. Biology Centre. Institute of Parasitology; República Checa; Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Aznar, Francisco J.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Blasco Costa, Isabel. Academy of Sciences of the Czech Republic. Biology Centre. Institute of Parasitology; República Checa; University of Otago. Department of Zoology; Nueva Zelanda;
Fil: Garcia, Nestor Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico. Laboratorio de Mamíferos Marinos; Argentina;
Fil: Víllora Montero, María. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Crespo, Enrique Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico. Laboratorio de Mamíferos Marinos; Argentina;
Fil: Raga, Juan A.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Montero. Francisco E.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Materia
ANISAKIDAE
SEALWORMS
PSEUDOTERRANOVA CATTANI
TAXONOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1629

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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, ArgentinaHernández Orts, Jesús ServandoAznar, Francisco J.Blasco Costa, IsabelGarcia, Nestor AnibalVíllora Montero, MaríaCrespo, Enrique AlbertoRaga, Juan A.Montero. Francisco E.ANISAKIDAESEALWORMSPSEUDOTERRANOVA CATTANITAXONOMYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Third-stage larvae of the Pseudoterranova decipiens species complex (also known as sealworms) have been reported in at least 40 marine fish species belonging to 21 families and 10 orders along the South American coast. Sealworms are a cause for concern because they can infect humans who consume raw or undercooked fish. However, despite their economic and zoonotic importance, morphological and molecular characterization of species of Pseudoterranova in South America is still scarce. Methods: A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. Results: A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver. Conclusions: We provided the first molecular identification, morphological description and microhabitat characterization of sealworm larvae from the Argentinean Patagonian coast. We also reported the infection levels of sealworms on 20 fish species in order to elucidate the life cycle of these nematodes in this area.Fil: Hernández Orts, Jesús Servando. Academy of Sciences of the Czech Republic. Biology Centre. Institute of Parasitology; República Checa; Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;Fil: Aznar, Francisco J.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;Fil: Blasco Costa, Isabel. Academy of Sciences of the Czech Republic. Biology Centre. Institute of Parasitology; República Checa; University of Otago. Department of Zoology; Nueva Zelanda;Fil: Garcia, Nestor Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico. Laboratorio de Mamíferos Marinos; Argentina;Fil: Víllora Montero, María. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;Fil: Crespo, Enrique Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico. Laboratorio de Mamíferos Marinos; Argentina;Fil: Raga, Juan A.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;Fil: Montero. Francisco E.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;Biomed Central2013-08-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1629Hernández Orts, Jesús Servando; Aznar, Francisco J.; Blasco Costa, Isabel; Garcia, Nestor Anibal; Víllora Montero, María; et al.; Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina; Biomed Central; Parasites and Vectors; 6; 252; 29-8-2013; 1-151756-3305enginfo:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847798/info:eu-repo/semantics/altIdentifier/url/http://www.parasitesandvectors.com/content/6/1/252info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:25:33Zoai:ri.conicet.gov.ar:11336/1629instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:25:34.081CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
title Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
spellingShingle Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
Hernández Orts, Jesús Servando
ANISAKIDAE
SEALWORMS
PSEUDOTERRANOVA CATTANI
TAXONOMY
title_short Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
title_full Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
title_fullStr Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
title_full_unstemmed Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
title_sort Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina
dc.creator.none.fl_str_mv Hernández Orts, Jesús Servando
Aznar, Francisco J.
Blasco Costa, Isabel
Garcia, Nestor Anibal
Víllora Montero, María
Crespo, Enrique Alberto
Raga, Juan A.
Montero. Francisco E.
author Hernández Orts, Jesús Servando
author_facet Hernández Orts, Jesús Servando
Aznar, Francisco J.
Blasco Costa, Isabel
Garcia, Nestor Anibal
Víllora Montero, María
Crespo, Enrique Alberto
Raga, Juan A.
Montero. Francisco E.
author_role author
author2 Aznar, Francisco J.
Blasco Costa, Isabel
Garcia, Nestor Anibal
Víllora Montero, María
Crespo, Enrique Alberto
Raga, Juan A.
Montero. Francisco E.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANISAKIDAE
SEALWORMS
PSEUDOTERRANOVA CATTANI
TAXONOMY
topic ANISAKIDAE
SEALWORMS
PSEUDOTERRANOVA CATTANI
TAXONOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Third-stage larvae of the Pseudoterranova decipiens species complex (also known as sealworms) have been reported in at least 40 marine fish species belonging to 21 families and 10 orders along the South American coast. Sealworms are a cause for concern because they can infect humans who consume raw or undercooked fish. However, despite their economic and zoonotic importance, morphological and molecular characterization of species of Pseudoterranova in South America is still scarce. Methods: A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. Results: A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver. Conclusions: We provided the first molecular identification, morphological description and microhabitat characterization of sealworm larvae from the Argentinean Patagonian coast. We also reported the infection levels of sealworms on 20 fish species in order to elucidate the life cycle of these nematodes in this area.
Fil: Hernández Orts, Jesús Servando. Academy of Sciences of the Czech Republic. Biology Centre. Institute of Parasitology; República Checa; Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Aznar, Francisco J.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Blasco Costa, Isabel. Academy of Sciences of the Czech Republic. Biology Centre. Institute of Parasitology; República Checa; University of Otago. Department of Zoology; Nueva Zelanda;
Fil: Garcia, Nestor Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico. Laboratorio de Mamíferos Marinos; Argentina;
Fil: Víllora Montero, María. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Crespo, Enrique Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico. Laboratorio de Mamíferos Marinos; Argentina;
Fil: Raga, Juan A.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
Fil: Montero. Francisco E.. Universidad de Valencia. Instituto Cavanilles de Biodiversidad y Biología Evolutiva; España;
description Third-stage larvae of the Pseudoterranova decipiens species complex (also known as sealworms) have been reported in at least 40 marine fish species belonging to 21 families and 10 orders along the South American coast. Sealworms are a cause for concern because they can infect humans who consume raw or undercooked fish. However, despite their economic and zoonotic importance, morphological and molecular characterization of species of Pseudoterranova in South America is still scarce. Methods: A total of 542 individual fish from 20 species from the Patagonian coast of Argentina were examined for sealworms. The body cavity, the muscles, internal organs, and the mesenteries were examined to detect nematodes. Sealworm larvae were removed from their capsules and fixed in 70% ethanol. For molecular identification, partial fragments of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1) were amplified for 10 isolates from 4 fish species. Morphological and morphometric data of sealworms were also obtained. Results: A total of 635 larvae were collected from 12 fish species. The most infected fish was Prionotus nudigula, followed by Percophis brasiliensis, Acanthistius patachonicus, Paralichthys isosceles, and Pseudopercis semifasciata. Sequences obtained for the cox1 of sealworms from A. patachonicus, P. isosceles, P. brasiliensis and P. nudigula formed a reciprocally monophyletic lineage with published sequences of adult specimens of Pseudoterranova cattani from the South American sea lion Otaria flavescens, and distinct from the remaining 5 species of Pseudoterranova. A morphological description, including drawings and scanning electron microscopy photomicrographs of these larvae is provided. Sealworms collected from Argentinean fishes did not differ in their diagnostic traits from the previously described larvae of P. cattani. However a discriminant analysis suggests that specimens from P. nudigula were significantly larger than those from other fishes. Most of the sealworms were collected encapsulated from the muscles and, to a lesser degree, from the mesenteries and the liver. Conclusions: We provided the first molecular identification, morphological description and microhabitat characterization of sealworm larvae from the Argentinean Patagonian coast. We also reported the infection levels of sealworms on 20 fish species in order to elucidate the life cycle of these nematodes in this area.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1629
Hernández Orts, Jesús Servando; Aznar, Francisco J.; Blasco Costa, Isabel; Garcia, Nestor Anibal; Víllora Montero, María; et al.; Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina; Biomed Central; Parasites and Vectors; 6; 252; 29-8-2013; 1-15
1756-3305
url http://hdl.handle.net/11336/1629
identifier_str_mv Hernández Orts, Jesús Servando; Aznar, Francisco J.; Blasco Costa, Isabel; Garcia, Nestor Anibal; Víllora Montero, María; et al.; Description, microhabitat selection and infection patterns of sealworm larvae (Pseudoterranova decipiens species complex, nematoda: ascaridoidea) in fishes from Patagonia, Argentina; Biomed Central; Parasites and Vectors; 6; 252; 29-8-2013; 1-15
1756-3305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3847798/
info:eu-repo/semantics/altIdentifier/url/http://www.parasitesandvectors.com/content/6/1/252
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
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