Colonization and plant growth-promotion of tomato by Burkholderia tropica

Autores
Bernabeu, Pamela Romina; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo; Estrada De los Santos, Paulina; Galar, Maria Lina; Boiardi, Jose Luis; Luna, Maria Flavia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several diazotrophic Burkholderia species have been described to exhibit some activities involved in plant growth promotion and biological control. In this work seedlings of tomato plants were inoculated with this bacterium in order to study colonization of different vegetal tissues and plant growth promoting ability under greenhouse conditions. Tomato seedlings inoculated with Burkholderia tropica strain MTo-293 and two derivative strains containing the marker genes gusA and gfp, respectively (constructions described in this work), were grown under gnotobiotic conditions. Colonization was monitored both by colony counting of bacterial suspensions from homogenized tissues with or without previous surface disinfection and by microscopic observation of entire plant tissues. In another set of experiments tomato seedlings were inoculated with B. tropica MTo-293 for evaluation of tomato production under greenhouse conditions. Tomato yields were determined by quantifying total tomato production throughout the crop in two different seasons. B. tropica could be isolated from root surfaces (>7.0 log CFU g−1 fresh weight) and from surface-disinfected and disrupted roots (>5.0 log CFU g−1 fresh weight) and stems (>4.0 log CFU g−1 fresh weight) of inoculated plants. Microscopic studies showed colonizing bacteria on root hairs, root tips, lateral root emergence sites, and stomata. In greenhouse experiments inoculated plants showed a consistent increase of both number and weight of fruits as compared to uninoculated controls. Although this enhancement in fruit production was only statistically significant for fruit weight in the first crop season, our results show a consistent tendency to a higher yield (5–15%) for the inoculated treatments also in the second year. These results show that seedling inoculation with B. tropica led to effective root colonization of tomato plants followed by bacterial spreading to aerial tissues. This significant colonization was accompanied by an enhancement of tomato production in two different crop seasons.
Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Estrada De los Santos, Paulina. Instituto Politecnico Nacional; México
Fil: Galar, Maria Lina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Luna, Maria Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
Burkholderia
Inoculation
Tomato
Colonization
Pgpb
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48021
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48021
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Colonization and plant growth-promotion of tomato by Burkholderia tropicaBernabeu, Pamela RominaPistorio, MarianoTorres Tejerizo, Gonzalo ArturoEstrada De los Santos, PaulinaGalar, Maria LinaBoiardi, Jose LuisLuna, Maria FlaviaBurkholderiaInoculationTomatoColonizationPgpbhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Several diazotrophic Burkholderia species have been described to exhibit some activities involved in plant growth promotion and biological control. In this work seedlings of tomato plants were inoculated with this bacterium in order to study colonization of different vegetal tissues and plant growth promoting ability under greenhouse conditions. Tomato seedlings inoculated with Burkholderia tropica strain MTo-293 and two derivative strains containing the marker genes gusA and gfp, respectively (constructions described in this work), were grown under gnotobiotic conditions. Colonization was monitored both by colony counting of bacterial suspensions from homogenized tissues with or without previous surface disinfection and by microscopic observation of entire plant tissues. In another set of experiments tomato seedlings were inoculated with B. tropica MTo-293 for evaluation of tomato production under greenhouse conditions. Tomato yields were determined by quantifying total tomato production throughout the crop in two different seasons. B. tropica could be isolated from root surfaces (>7.0 log CFU g−1 fresh weight) and from surface-disinfected and disrupted roots (>5.0 log CFU g−1 fresh weight) and stems (>4.0 log CFU g−1 fresh weight) of inoculated plants. Microscopic studies showed colonizing bacteria on root hairs, root tips, lateral root emergence sites, and stomata. In greenhouse experiments inoculated plants showed a consistent increase of both number and weight of fruits as compared to uninoculated controls. Although this enhancement in fruit production was only statistically significant for fruit weight in the first crop season, our results show a consistent tendency to a higher yield (5–15%) for the inoculated treatments also in the second year. These results show that seedling inoculation with B. tropica led to effective root colonization of tomato plants followed by bacterial spreading to aerial tissues. This significant colonization was accompanied by an enhancement of tomato production in two different crop seasons.Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Estrada De los Santos, Paulina. Instituto Politecnico Nacional; MéxicoFil: Galar, Maria Lina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Luna, Maria Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier Science2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48021Bernabeu, Pamela Romina; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo; Estrada De los Santos, Paulina; Galar, Maria Lina; et al.; Colonization and plant growth-promotion of tomato by Burkholderia tropica; Elsevier Science; Scientia Horticulturae; 191; 8-2015; 113-1200304-4238CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304423815002629info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2015.05.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:42Zoai:ri.conicet.gov.ar:11336/48021instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:42.69CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Colonization and plant growth-promotion of tomato by Burkholderia tropica
title Colonization and plant growth-promotion of tomato by Burkholderia tropica
spellingShingle Colonization and plant growth-promotion of tomato by Burkholderia tropica
Bernabeu, Pamela Romina
Burkholderia
Inoculation
Tomato
Colonization
Pgpb
title_short Colonization and plant growth-promotion of tomato by Burkholderia tropica
title_full Colonization and plant growth-promotion of tomato by Burkholderia tropica
title_fullStr Colonization and plant growth-promotion of tomato by Burkholderia tropica
title_full_unstemmed Colonization and plant growth-promotion of tomato by Burkholderia tropica
title_sort Colonization and plant growth-promotion of tomato by Burkholderia tropica
dc.creator.none.fl_str_mv Bernabeu, Pamela Romina
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
Estrada De los Santos, Paulina
Galar, Maria Lina
Boiardi, Jose Luis
Luna, Maria Flavia
author Bernabeu, Pamela Romina
author_facet Bernabeu, Pamela Romina
Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
Estrada De los Santos, Paulina
Galar, Maria Lina
Boiardi, Jose Luis
Luna, Maria Flavia
author_role author
author2 Pistorio, Mariano
Torres Tejerizo, Gonzalo Arturo
Estrada De los Santos, Paulina
Galar, Maria Lina
Boiardi, Jose Luis
Luna, Maria Flavia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Burkholderia
Inoculation
Tomato
Colonization
Pgpb
topic Burkholderia
Inoculation
Tomato
Colonization
Pgpb
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Several diazotrophic Burkholderia species have been described to exhibit some activities involved in plant growth promotion and biological control. In this work seedlings of tomato plants were inoculated with this bacterium in order to study colonization of different vegetal tissues and plant growth promoting ability under greenhouse conditions. Tomato seedlings inoculated with Burkholderia tropica strain MTo-293 and two derivative strains containing the marker genes gusA and gfp, respectively (constructions described in this work), were grown under gnotobiotic conditions. Colonization was monitored both by colony counting of bacterial suspensions from homogenized tissues with or without previous surface disinfection and by microscopic observation of entire plant tissues. In another set of experiments tomato seedlings were inoculated with B. tropica MTo-293 for evaluation of tomato production under greenhouse conditions. Tomato yields were determined by quantifying total tomato production throughout the crop in two different seasons. B. tropica could be isolated from root surfaces (>7.0 log CFU g−1 fresh weight) and from surface-disinfected and disrupted roots (>5.0 log CFU g−1 fresh weight) and stems (>4.0 log CFU g−1 fresh weight) of inoculated plants. Microscopic studies showed colonizing bacteria on root hairs, root tips, lateral root emergence sites, and stomata. In greenhouse experiments inoculated plants showed a consistent increase of both number and weight of fruits as compared to uninoculated controls. Although this enhancement in fruit production was only statistically significant for fruit weight in the first crop season, our results show a consistent tendency to a higher yield (5–15%) for the inoculated treatments also in the second year. These results show that seedling inoculation with B. tropica led to effective root colonization of tomato plants followed by bacterial spreading to aerial tissues. This significant colonization was accompanied by an enhancement of tomato production in two different crop seasons.
Fil: Bernabeu, Pamela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Torres Tejerizo, Gonzalo Arturo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Estrada De los Santos, Paulina. Instituto Politecnico Nacional; México
Fil: Galar, Maria Lina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Luna, Maria Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description Several diazotrophic Burkholderia species have been described to exhibit some activities involved in plant growth promotion and biological control. In this work seedlings of tomato plants were inoculated with this bacterium in order to study colonization of different vegetal tissues and plant growth promoting ability under greenhouse conditions. Tomato seedlings inoculated with Burkholderia tropica strain MTo-293 and two derivative strains containing the marker genes gusA and gfp, respectively (constructions described in this work), were grown under gnotobiotic conditions. Colonization was monitored both by colony counting of bacterial suspensions from homogenized tissues with or without previous surface disinfection and by microscopic observation of entire plant tissues. In another set of experiments tomato seedlings were inoculated with B. tropica MTo-293 for evaluation of tomato production under greenhouse conditions. Tomato yields were determined by quantifying total tomato production throughout the crop in two different seasons. B. tropica could be isolated from root surfaces (>7.0 log CFU g−1 fresh weight) and from surface-disinfected and disrupted roots (>5.0 log CFU g−1 fresh weight) and stems (>4.0 log CFU g−1 fresh weight) of inoculated plants. Microscopic studies showed colonizing bacteria on root hairs, root tips, lateral root emergence sites, and stomata. In greenhouse experiments inoculated plants showed a consistent increase of both number and weight of fruits as compared to uninoculated controls. Although this enhancement in fruit production was only statistically significant for fruit weight in the first crop season, our results show a consistent tendency to a higher yield (5–15%) for the inoculated treatments also in the second year. These results show that seedling inoculation with B. tropica led to effective root colonization of tomato plants followed by bacterial spreading to aerial tissues. This significant colonization was accompanied by an enhancement of tomato production in two different crop seasons.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48021
Bernabeu, Pamela Romina; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo; Estrada De los Santos, Paulina; Galar, Maria Lina; et al.; Colonization and plant growth-promotion of tomato by Burkholderia tropica; Elsevier Science; Scientia Horticulturae; 191; 8-2015; 113-120
0304-4238
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48021
identifier_str_mv Bernabeu, Pamela Romina; Pistorio, Mariano; Torres Tejerizo, Gonzalo Arturo; Estrada De los Santos, Paulina; Galar, Maria Lina; et al.; Colonization and plant growth-promotion of tomato by Burkholderia tropica; Elsevier Science; Scientia Horticulturae; 191; 8-2015; 113-120
0304-4238
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304423815002629
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scienta.2015.05.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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