Preliminary assessment of in vivo safety of potentially probiotic lactic acid bacteria for American bullfrog culture

Authors
Pasteris, Sergio Enrique; Montel Mendoza, María Gabriela; Llanos, Ricardo J.; Pucci Alcaide, Franco J.; Nader, Maria Elena Fatima
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
The effect of the administration of beneficial lactic acid bacteria (LAB): Lactococcus lactis CRL 1584, L. lactis CRL 1827, Enterococcus gallinarum CRL 1826 and combined CRL 1584+ CRL 1826 on the development of bullfrog embryos from the hatching stage until 31 days (tadpole) was evaluated. In vitro assays indicated that 103, 106 and 109 CFU mL−1 single LAB strains remained viable until 24 h in 10% Ringer. Around 109 CFU mL−1 LAB (individually and combined) were used in an experimental design built to evaluate their effect when administrated at different intervals (three 7-day cycles with 5-day rest periods in between) to embryos until day 31. The highest potentially beneficial population (LAB) numbers were detected in the LAB-treated groups. All the LAB-treatments increased it and were significantly higher than the controls. Although the highest, potentially pathogenic, population (Red-Leg Syndrome-RLS-related pathogens) numbers were detected in the control and the lowest in the CRL 1584+ CRL 1826-treated group, they did not differ significantly. Stereoscopic studies showed no malformations in any LAB-treated group and all the specimens reached the same stage of their biological cycle with a survival >94%. The histological structure of target organs for RLS-associated pathogens (intestine and skin) and stomach was not affected and the spleen was developed. Only the LAB-treated groups showed microorganisms associated with the intestinal mucus, without inflammatory reaction in the lamina propria. This is the first report on the evaluation of the absence of adverse effects after LAB administration to bullfrog embryos using indigenous microorganisms.
Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Montel Mendoza, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Llanos, Ricardo J.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pucci Alcaide, Franco J.. Fundación Miguel Lillo; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Subject
BULLFROG CULTURE
LACTIC ACID BACTERIA
MORPHOLOGICAL STUDIES
PROBIOTICS
RANICULTURE
Agricultura
Agricultura, Silvicultura y Pesca
CIENCIAS AGRÍCOLAS
Access level
Restricted access
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/36723