Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination

Autores
Damiani, Maria Cecilia; Leonardi, Patricia Ines; Pieroni, Olga Inés; Caceres, Eduardo Jorge
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The cell wall structure and behaviour of cysts of Argentinian strains of Haematococcus pluvialis were studied during cyst germination and zooid release. The cyst wall is composed of an outer primary wall, a trilaminar sheath, a secondary wall, and a tertiary wall. The primary wall disintegrates as the cyst ages. During germination the trilaminar sheath breaks down, carrying the adjacent secondary wall with it and the extensible tertiary wall accompanies the cyst in its size increase and shape changes. Zooid release subsequently follows as a result of the apical breakage of this third wall. Although the secondary and tertiary walls exhibit the same conformation in mature cysts, the arrangement of their components during their formation is different. This could be the reason why the behaviour of the two walls is different during germination. The chemical characterization carried out in the present research showed that the external trilaminar sheath of the wall is formed by algaenan, while fluorescence labelling suggested the presence of mannose and cellulose in the secondary and tertiary walls. Because of the physical difficulty of breaking cysts during astaxanthin extraction, cyst germination may provide a natural, alternative wall-breaking mechanism.
Fil: Damiani, Maria Cecilia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pieroni, Olga Inés. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Caceres, Eduardo Jorge. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
Materia
Algaenan, , , , , , ,
Ultrastructure
Astaxanthin
Cell Wall
Chlorophyceae
Cyst
Germination
Haematococcus Pluvialis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34467
Acceder
id CONICETDig_852ff0230aff38f9d394cf820cad924c
oai_identifier_str oai:ri.conicet.gov.ar:11336/34467
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germinationDamiani, Maria CeciliaLeonardi, Patricia InesPieroni, Olga InésCaceres, Eduardo JorgeAlgaenan, , , , , , ,UltrastructureAstaxanthinCell WallChlorophyceaeCystGerminationHaematococcus Pluvialishttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The cell wall structure and behaviour of cysts of Argentinian strains of Haematococcus pluvialis were studied during cyst germination and zooid release. The cyst wall is composed of an outer primary wall, a trilaminar sheath, a secondary wall, and a tertiary wall. The primary wall disintegrates as the cyst ages. During germination the trilaminar sheath breaks down, carrying the adjacent secondary wall with it and the extensible tertiary wall accompanies the cyst in its size increase and shape changes. Zooid release subsequently follows as a result of the apical breakage of this third wall. Although the secondary and tertiary walls exhibit the same conformation in mature cysts, the arrangement of their components during their formation is different. This could be the reason why the behaviour of the two walls is different during germination. The chemical characterization carried out in the present research showed that the external trilaminar sheath of the wall is formed by algaenan, while fluorescence labelling suggested the presence of mannose and cellulose in the secondary and tertiary walls. Because of the physical difficulty of breaking cysts during astaxanthin extraction, cyst germination may provide a natural, alternative wall-breaking mechanism.Fil: Damiani, Maria Cecilia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Pieroni, Olga Inés. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Caceres, Eduardo Jorge. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; ArgentinaInternational Phycological Society2006-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34467Damiani, Maria Cecilia; Leonardi, Patricia Ines; Pieroni, Olga Inés; Caceres, Eduardo Jorge; Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination; International Phycological Society; Phycologia; 45; 6; 11-2006; 616-6230031-8884CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.phycologia.org/doi/abs/10.2216/05-27.1info:eu-repo/semantics/altIdentifier/doi/10.2216/05-27.1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:23:16Zoai:ri.conicet.gov.ar:11336/34467instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:23:16.883CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
title Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
spellingShingle Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
Damiani, Maria Cecilia
Algaenan, , , , , , ,
Ultrastructure
Astaxanthin
Cell Wall
Chlorophyceae
Cyst
Germination
Haematococcus Pluvialis
title_short Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
title_full Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
title_fullStr Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
title_full_unstemmed Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
title_sort Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination
dc.creator.none.fl_str_mv Damiani, Maria Cecilia
Leonardi, Patricia Ines
Pieroni, Olga Inés
Caceres, Eduardo Jorge
author Damiani, Maria Cecilia
author_facet Damiani, Maria Cecilia
Leonardi, Patricia Ines
Pieroni, Olga Inés
Caceres, Eduardo Jorge
author_role author
author2 Leonardi, Patricia Ines
Pieroni, Olga Inés
Caceres, Eduardo Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv Algaenan, , , , , , ,
Ultrastructure
Astaxanthin
Cell Wall
Chlorophyceae
Cyst
Germination
Haematococcus Pluvialis
topic Algaenan, , , , , , ,
Ultrastructure
Astaxanthin
Cell Wall
Chlorophyceae
Cyst
Germination
Haematococcus Pluvialis
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The cell wall structure and behaviour of cysts of Argentinian strains of Haematococcus pluvialis were studied during cyst germination and zooid release. The cyst wall is composed of an outer primary wall, a trilaminar sheath, a secondary wall, and a tertiary wall. The primary wall disintegrates as the cyst ages. During germination the trilaminar sheath breaks down, carrying the adjacent secondary wall with it and the extensible tertiary wall accompanies the cyst in its size increase and shape changes. Zooid release subsequently follows as a result of the apical breakage of this third wall. Although the secondary and tertiary walls exhibit the same conformation in mature cysts, the arrangement of their components during their formation is different. This could be the reason why the behaviour of the two walls is different during germination. The chemical characterization carried out in the present research showed that the external trilaminar sheath of the wall is formed by algaenan, while fluorescence labelling suggested the presence of mannose and cellulose in the secondary and tertiary walls. Because of the physical difficulty of breaking cysts during astaxanthin extraction, cyst germination may provide a natural, alternative wall-breaking mechanism.
Fil: Damiani, Maria Cecilia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pieroni, Olga Inés. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Caceres, Eduardo Jorge. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
description The cell wall structure and behaviour of cysts of Argentinian strains of Haematococcus pluvialis were studied during cyst germination and zooid release. The cyst wall is composed of an outer primary wall, a trilaminar sheath, a secondary wall, and a tertiary wall. The primary wall disintegrates as the cyst ages. During germination the trilaminar sheath breaks down, carrying the adjacent secondary wall with it and the extensible tertiary wall accompanies the cyst in its size increase and shape changes. Zooid release subsequently follows as a result of the apical breakage of this third wall. Although the secondary and tertiary walls exhibit the same conformation in mature cysts, the arrangement of their components during their formation is different. This could be the reason why the behaviour of the two walls is different during germination. The chemical characterization carried out in the present research showed that the external trilaminar sheath of the wall is formed by algaenan, while fluorescence labelling suggested the presence of mannose and cellulose in the secondary and tertiary walls. Because of the physical difficulty of breaking cysts during astaxanthin extraction, cyst germination may provide a natural, alternative wall-breaking mechanism.
publishDate 2006
dc.date.none.fl_str_mv 2006-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34467
Damiani, Maria Cecilia; Leonardi, Patricia Ines; Pieroni, Olga Inés; Caceres, Eduardo Jorge; Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination; International Phycological Society; Phycologia; 45; 6; 11-2006; 616-623
0031-8884
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34467
identifier_str_mv Damiani, Maria Cecilia; Leonardi, Patricia Ines; Pieroni, Olga Inés; Caceres, Eduardo Jorge; Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination; International Phycological Society; Phycologia; 45; 6; 11-2006; 616-623
0031-8884
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.phycologia.org/doi/abs/10.2216/05-27.1
info:eu-repo/semantics/altIdentifier/doi/10.2216/05-27.1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv International Phycological Society
publisher.none.fl_str_mv International Phycological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614227112755200
score 13.070432

Publicaciones similares