Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays

Autores
Eberhardt, María Ayelen Teresita; Monje, Lucas Daniel; Zurvera, D. A.; Beldomenico, Pablo Martín
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma evansi is a flagellated protozoan that parasitizes a wide variety of mammals, occasionally including humans. In South America, it infects horses, cattle, buffaloes, dogs and wild mammals, causing a disease known as "Mal de Caderas", which results in important economic losses due to a wide range of pathological expressions. Argentina represents the southern limit of its distribution. The capybara (Hydrochoerus hydrochaeris) is a large rodent found in tropical to temperate freshwater wetlands of South America. As capybaras infected with T. evansi present no clinical signs of disease, withstanding high parasitaemia, this species was proposed as a reservoir host. In this study we investigated the prevalence and parasitaemic intensity of T. evansi in samples obtained from 60 free-ranging capybaras of Esteros del Iberá (Corrientes province, northeastern Argentina) using smear microscopy and real-time PCR assays. All the cases of capybaras infected with T. evansi were found during one of the years studied, with no evidence of seasonality. The overall infection prevalence was 10%, but between years it ranged from 0% to 17% (in 2011). This is the first confirmation of T. evansi infection in Argentina by molecular biology techniques. Our results showed no differences between the methods used to detect the presence of T. evansi in capybaras, which indicates that simple methods like microscopy can generate important data on the ecoepidemiology of this parasite. Both techniques used in this study represent a viable tool for ecoepidemiological studies, and can be used to produce good estimates of prevalence and parasitaemic level of the infection, which inform for the implementation of strategies for the control of the disease.
Fil: Eberhardt, María Ayelen Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Monje, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zurvera, D. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Materia
Trypanosoma Evansi
Mal de Caderas
Surra
Hydrochoerus Hydrochaeris
Capybara
Smear Microscopy
Real Time Pcr
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/20295
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/20295
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assaysEberhardt, María Ayelen TeresitaMonje, Lucas DanielZurvera, D. A.Beldomenico, Pablo MartínTrypanosoma EvansiMal de CaderasSurraHydrochoerus HydrochaerisCapybaraSmear MicroscopyReal Time Pcrhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Trypanosoma evansi is a flagellated protozoan that parasitizes a wide variety of mammals, occasionally including humans. In South America, it infects horses, cattle, buffaloes, dogs and wild mammals, causing a disease known as "Mal de Caderas", which results in important economic losses due to a wide range of pathological expressions. Argentina represents the southern limit of its distribution. The capybara (Hydrochoerus hydrochaeris) is a large rodent found in tropical to temperate freshwater wetlands of South America. As capybaras infected with T. evansi present no clinical signs of disease, withstanding high parasitaemia, this species was proposed as a reservoir host. In this study we investigated the prevalence and parasitaemic intensity of T. evansi in samples obtained from 60 free-ranging capybaras of Esteros del Iberá (Corrientes province, northeastern Argentina) using smear microscopy and real-time PCR assays. All the cases of capybaras infected with T. evansi were found during one of the years studied, with no evidence of seasonality. The overall infection prevalence was 10%, but between years it ranged from 0% to 17% (in 2011). This is the first confirmation of T. evansi infection in Argentina by molecular biology techniques. Our results showed no differences between the methods used to detect the presence of T. evansi in capybaras, which indicates that simple methods like microscopy can generate important data on the ecoepidemiology of this parasite. Both techniques used in this study represent a viable tool for ecoepidemiological studies, and can be used to produce good estimates of prevalence and parasitaemic level of the infection, which inform for the implementation of strategies for the control of the disease.Fil: Eberhardt, María Ayelen Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Monje, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Zurvera, D. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaElsevier2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/20295Eberhardt, María Ayelen Teresita; Monje, Lucas Daniel; Zurvera, D. A.; Beldomenico, Pablo Martín; Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays; Elsevier; Veterinary Parasitology; 202; 3-4; 5-2014; 226-2330304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2014.02.043info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0304401714001307?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:14Zoai:ri.conicet.gov.ar:11336/20295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:15.01CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
title Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
spellingShingle Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
Eberhardt, María Ayelen Teresita
Trypanosoma Evansi
Mal de Caderas
Surra
Hydrochoerus Hydrochaeris
Capybara
Smear Microscopy
Real Time Pcr
title_short Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
title_full Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
title_fullStr Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
title_full_unstemmed Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
title_sort Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays
dc.creator.none.fl_str_mv Eberhardt, María Ayelen Teresita
Monje, Lucas Daniel
Zurvera, D. A.
Beldomenico, Pablo Martín
author Eberhardt, María Ayelen Teresita
author_facet Eberhardt, María Ayelen Teresita
Monje, Lucas Daniel
Zurvera, D. A.
Beldomenico, Pablo Martín
author_role author
author2 Monje, Lucas Daniel
Zurvera, D. A.
Beldomenico, Pablo Martín
author2_role author
author
author
dc.subject.none.fl_str_mv Trypanosoma Evansi
Mal de Caderas
Surra
Hydrochoerus Hydrochaeris
Capybara
Smear Microscopy
Real Time Pcr
topic Trypanosoma Evansi
Mal de Caderas
Surra
Hydrochoerus Hydrochaeris
Capybara
Smear Microscopy
Real Time Pcr
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Trypanosoma evansi is a flagellated protozoan that parasitizes a wide variety of mammals, occasionally including humans. In South America, it infects horses, cattle, buffaloes, dogs and wild mammals, causing a disease known as "Mal de Caderas", which results in important economic losses due to a wide range of pathological expressions. Argentina represents the southern limit of its distribution. The capybara (Hydrochoerus hydrochaeris) is a large rodent found in tropical to temperate freshwater wetlands of South America. As capybaras infected with T. evansi present no clinical signs of disease, withstanding high parasitaemia, this species was proposed as a reservoir host. In this study we investigated the prevalence and parasitaemic intensity of T. evansi in samples obtained from 60 free-ranging capybaras of Esteros del Iberá (Corrientes province, northeastern Argentina) using smear microscopy and real-time PCR assays. All the cases of capybaras infected with T. evansi were found during one of the years studied, with no evidence of seasonality. The overall infection prevalence was 10%, but between years it ranged from 0% to 17% (in 2011). This is the first confirmation of T. evansi infection in Argentina by molecular biology techniques. Our results showed no differences between the methods used to detect the presence of T. evansi in capybaras, which indicates that simple methods like microscopy can generate important data on the ecoepidemiology of this parasite. Both techniques used in this study represent a viable tool for ecoepidemiological studies, and can be used to produce good estimates of prevalence and parasitaemic level of the infection, which inform for the implementation of strategies for the control of the disease.
Fil: Eberhardt, María Ayelen Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Monje, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zurvera, D. A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Cs.veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
description Trypanosoma evansi is a flagellated protozoan that parasitizes a wide variety of mammals, occasionally including humans. In South America, it infects horses, cattle, buffaloes, dogs and wild mammals, causing a disease known as "Mal de Caderas", which results in important economic losses due to a wide range of pathological expressions. Argentina represents the southern limit of its distribution. The capybara (Hydrochoerus hydrochaeris) is a large rodent found in tropical to temperate freshwater wetlands of South America. As capybaras infected with T. evansi present no clinical signs of disease, withstanding high parasitaemia, this species was proposed as a reservoir host. In this study we investigated the prevalence and parasitaemic intensity of T. evansi in samples obtained from 60 free-ranging capybaras of Esteros del Iberá (Corrientes province, northeastern Argentina) using smear microscopy and real-time PCR assays. All the cases of capybaras infected with T. evansi were found during one of the years studied, with no evidence of seasonality. The overall infection prevalence was 10%, but between years it ranged from 0% to 17% (in 2011). This is the first confirmation of T. evansi infection in Argentina by molecular biology techniques. Our results showed no differences between the methods used to detect the presence of T. evansi in capybaras, which indicates that simple methods like microscopy can generate important data on the ecoepidemiology of this parasite. Both techniques used in this study represent a viable tool for ecoepidemiological studies, and can be used to produce good estimates of prevalence and parasitaemic level of the infection, which inform for the implementation of strategies for the control of the disease.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/20295
Eberhardt, María Ayelen Teresita; Monje, Lucas Daniel; Zurvera, D. A.; Beldomenico, Pablo Martín; Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays; Elsevier; Veterinary Parasitology; 202; 3-4; 5-2014; 226-233
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/20295
identifier_str_mv Eberhardt, María Ayelen Teresita; Monje, Lucas Daniel; Zurvera, D. A.; Beldomenico, Pablo Martín; Detection of Trypanosoma evansi infection in wild capybaras from Argentina using smear microscopy and real-time PCR assays; Elsevier; Veterinary Parasitology; 202; 3-4; 5-2014; 226-233
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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