Enhanced volatile phenols in wine fermented with Saccharomyces cerevisiae and spoiled with Pichia guilliermondii and Dekkera bruxellensis

• Autores: Sáez, J. S.; Lopes, Christian Ariel; Kirs, V. C.; Sangorrín, M. P.
• Año de publicación: 2010
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: DEKKERA BRUXELLENSIS; ETHYLPHENOLS; FERULIC ACID; P-COUMARIC ACID; PICHIA GUILLIEROMONDII; SPOILAGE WINE; VINYLPHENOLS
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Aims: To investigate whether the presence of Pichia guilliermondii impacts on theproduction of volatile phenols from mixed wine fermentations with Dekkerabruxellensis and Saccharomyces cerevisiae.Methods and Results: Four inoculation strategies were performed in small-scalefermentations involving P. guilliermondii, D. bruxellensis and S. cerevisiae usingSyrah grape juice supplemented with 100 mg l)1 of p-coumaric acid. Highpressure liquid chromatography was used for the quantification or volatile phenols.Significant high levels of 4-ethylphenol and 4-ethylguaicol (720 and545 lg l)1, respectively), as well as the highest levels of 4-vinylphenol(>4500 lg l)1), were observed when P. guilliermondii species was inoculatedfrom the beginning of the fermentation.Conclusions: The metabolic interaction occurring between the high vinylphenolproducer species P. guilliermondii and D. bruxellensis exhibiting a high vinylphenolreductase activity resulted in an increased production of volatilephenols in wine.Significance and Impact of the Study: Pichia guilliermondii must be considereda very important spoilage yeast in the wine industry capable of producing largeamounts of volatile phenols.
Fil: Sáez, J. S.. No especifíca;
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
Fil: Kirs, V. C.. No especifíca;
Fil: Sangorrín, M. P.. No especifíca;