Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing

Autores
Bucci, Paula Lorena; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Zaritzky, Noemi Elisabet; Caravelli, Alejandro Horacio
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several nitrogen transformation processes occur in wastewater treatment; among them, heterotrophic nitrification-aerobic denitrification (HNAD) has not been adequately quantified. In the present study, a comprehensive analysis of the simultaneous ammonia and organic carbon removal was carried out in an aerobic granular sequencing batch reactor (SBR) operated under feast/famine regime. For this, biological processes, microbial community composition and metabolic pathways were evaluated. Mass balances allowed estimating the ammonia assimilation and simultaneous nitrification and denitrification (SND). Better SND process (55%) was achieved in starvation period involving that denitrification driven by intracellular carbon reserves is more efficiently coupled to the ammonia oxidation with regard to external carbon-driven denitrification. Proton balances and titration monitored the organic carbon and nitrogen removal. High abundance of HNAD bacteria, Diaphorobacter sp. and Flavobacterium sp., and Zoogloea sp. aerobic denitrifier was detected by next generation sequencing (NGS) technology. Bioinformatics analysis predicted enzymes involved in nitrogen transformation pathways present in Phylogenetic investigation PYCRUSt database. Heterotrophic nitrification took place involving detoxification against ammonia. Non-limiting nitrate and high oxygen concentrations were favorable for aerobic denitrifiers. HNAD was the main process responsible for the biological nitrogen removal according to NGS and operational conditions given by high dissolved oxygen and low COD:NO3−-N ratio.
Fil: Bucci, Paula Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Zaritzky, Noemi Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Materia
AEROBIC DENITRIFICATION
BACTERIAL COMPOSITION
HETEROTROPHIC NITRIFICATION
NEXT GENERATION SEQUENCING
ONLINE MONITORING
PYCRUST
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/173634
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencingBucci, Paula LorenaCoppotelli, Bibiana MarinaMorelli, Irma SusanaZaritzky, Noemi ElisabetCaravelli, Alejandro HoracioAEROBIC DENITRIFICATIONBACTERIAL COMPOSITIONHETEROTROPHIC NITRIFICATIONNEXT GENERATION SEQUENCINGONLINE MONITORINGPYCRUSThttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Several nitrogen transformation processes occur in wastewater treatment; among them, heterotrophic nitrification-aerobic denitrification (HNAD) has not been adequately quantified. In the present study, a comprehensive analysis of the simultaneous ammonia and organic carbon removal was carried out in an aerobic granular sequencing batch reactor (SBR) operated under feast/famine regime. For this, biological processes, microbial community composition and metabolic pathways were evaluated. Mass balances allowed estimating the ammonia assimilation and simultaneous nitrification and denitrification (SND). Better SND process (55%) was achieved in starvation period involving that denitrification driven by intracellular carbon reserves is more efficiently coupled to the ammonia oxidation with regard to external carbon-driven denitrification. Proton balances and titration monitored the organic carbon and nitrogen removal. High abundance of HNAD bacteria, Diaphorobacter sp. and Flavobacterium sp., and Zoogloea sp. aerobic denitrifier was detected by next generation sequencing (NGS) technology. Bioinformatics analysis predicted enzymes involved in nitrogen transformation pathways present in Phylogenetic investigation PYCRUSt database. Heterotrophic nitrification took place involving detoxification against ammonia. Non-limiting nitrate and high oxygen concentrations were favorable for aerobic denitrifiers. HNAD was the main process responsible for the biological nitrogen removal according to NGS and operational conditions given by high dissolved oxygen and low COD:NO3−-N ratio.Fil: Bucci, Paula Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Zaritzky, Noemi Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaElsevier2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/173634Bucci, Paula Lorena; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Zaritzky, Noemi Elisabet; Caravelli, Alejandro Horacio; Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing; Elsevier; International Biodeterioration and Biodegradation; 160; 105210; 5-2021; 1-120964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2021.105210info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830521000408info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:48Zoai:ri.conicet.gov.ar:11336/173634instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:48.407CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
title Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
spellingShingle Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
Bucci, Paula Lorena
AEROBIC DENITRIFICATION
BACTERIAL COMPOSITION
HETEROTROPHIC NITRIFICATION
NEXT GENERATION SEQUENCING
ONLINE MONITORING
PYCRUST
title_short Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
title_full Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
title_fullStr Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
title_full_unstemmed Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
title_sort Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing
dc.creator.none.fl_str_mv Bucci, Paula Lorena
Coppotelli, Bibiana Marina
Morelli, Irma Susana
Zaritzky, Noemi Elisabet
Caravelli, Alejandro Horacio
author Bucci, Paula Lorena
author_facet Bucci, Paula Lorena
Coppotelli, Bibiana Marina
Morelli, Irma Susana
Zaritzky, Noemi Elisabet
Caravelli, Alejandro Horacio
author_role author
author2 Coppotelli, Bibiana Marina
Morelli, Irma Susana
Zaritzky, Noemi Elisabet
Caravelli, Alejandro Horacio
author2_role author
author
author
author
dc.subject.none.fl_str_mv AEROBIC DENITRIFICATION
BACTERIAL COMPOSITION
HETEROTROPHIC NITRIFICATION
NEXT GENERATION SEQUENCING
ONLINE MONITORING
PYCRUST
topic AEROBIC DENITRIFICATION
BACTERIAL COMPOSITION
HETEROTROPHIC NITRIFICATION
NEXT GENERATION SEQUENCING
ONLINE MONITORING
PYCRUST
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Several nitrogen transformation processes occur in wastewater treatment; among them, heterotrophic nitrification-aerobic denitrification (HNAD) has not been adequately quantified. In the present study, a comprehensive analysis of the simultaneous ammonia and organic carbon removal was carried out in an aerobic granular sequencing batch reactor (SBR) operated under feast/famine regime. For this, biological processes, microbial community composition and metabolic pathways were evaluated. Mass balances allowed estimating the ammonia assimilation and simultaneous nitrification and denitrification (SND). Better SND process (55%) was achieved in starvation period involving that denitrification driven by intracellular carbon reserves is more efficiently coupled to the ammonia oxidation with regard to external carbon-driven denitrification. Proton balances and titration monitored the organic carbon and nitrogen removal. High abundance of HNAD bacteria, Diaphorobacter sp. and Flavobacterium sp., and Zoogloea sp. aerobic denitrifier was detected by next generation sequencing (NGS) technology. Bioinformatics analysis predicted enzymes involved in nitrogen transformation pathways present in Phylogenetic investigation PYCRUSt database. Heterotrophic nitrification took place involving detoxification against ammonia. Non-limiting nitrate and high oxygen concentrations were favorable for aerobic denitrifiers. HNAD was the main process responsible for the biological nitrogen removal according to NGS and operational conditions given by high dissolved oxygen and low COD:NO3−-N ratio.
Fil: Bucci, Paula Lorena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Coppotelli, Bibiana Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Morelli, Irma Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Zaritzky, Noemi Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Caravelli, Alejandro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
description Several nitrogen transformation processes occur in wastewater treatment; among them, heterotrophic nitrification-aerobic denitrification (HNAD) has not been adequately quantified. In the present study, a comprehensive analysis of the simultaneous ammonia and organic carbon removal was carried out in an aerobic granular sequencing batch reactor (SBR) operated under feast/famine regime. For this, biological processes, microbial community composition and metabolic pathways were evaluated. Mass balances allowed estimating the ammonia assimilation and simultaneous nitrification and denitrification (SND). Better SND process (55%) was achieved in starvation period involving that denitrification driven by intracellular carbon reserves is more efficiently coupled to the ammonia oxidation with regard to external carbon-driven denitrification. Proton balances and titration monitored the organic carbon and nitrogen removal. High abundance of HNAD bacteria, Diaphorobacter sp. and Flavobacterium sp., and Zoogloea sp. aerobic denitrifier was detected by next generation sequencing (NGS) technology. Bioinformatics analysis predicted enzymes involved in nitrogen transformation pathways present in Phylogenetic investigation PYCRUSt database. Heterotrophic nitrification took place involving detoxification against ammonia. Non-limiting nitrate and high oxygen concentrations were favorable for aerobic denitrifiers. HNAD was the main process responsible for the biological nitrogen removal according to NGS and operational conditions given by high dissolved oxygen and low COD:NO3−-N ratio.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/173634
Bucci, Paula Lorena; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Zaritzky, Noemi Elisabet; Caravelli, Alejandro Horacio; Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing; Elsevier; International Biodeterioration and Biodegradation; 160; 105210; 5-2021; 1-12
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/173634
identifier_str_mv Bucci, Paula Lorena; Coppotelli, Bibiana Marina; Morelli, Irma Susana; Zaritzky, Noemi Elisabet; Caravelli, Alejandro Horacio; Heterotrophic nitrification-aerobic denitrification performance in a granular sequencing batch reactor supported by next generation sequencing; Elsevier; International Biodeterioration and Biodegradation; 160; 105210; 5-2021; 1-12
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2021.105210
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830521000408
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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