Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis

Autores
Clop, Eduardo Matias; Luna, Agustin; Turina, Anahi del Valle
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The proposed molecule with therapeutic effects in C. sativa oils, cannabidiol (CBD), interacts with the CB1 receptor and modulates its activity, although it has been proposed that its interaction is more complex and less direct than that of tetrahidrocannabinol (THC). CBD has been observed to interact with the serotonin 5-HT1A receptor, influence GABAergic signaling and modulate the activity of enzymes and transporters. Given that CBD is a lipophilic compound, the study of non-receptor mediated interaction with biological membranes could contribute to the understanding of its mechanism of action.This research focused on analyzing the effect of a CBD-rich resin (CBD=92%, THC= 4%) on membrane models, with different degrees of complexity, by means of Langmuir films (LF) and fluorescence spectroscopy. LF were built from purified membranes of bovine brain synaptosomes (SM), as well as their lipid fraction (LF) and pure POPC phospholipids. Compression isotherms surface pressure () vs area, in the presence and absence of the resin (R) were performed for the three model membranes. Penetration experiments of R from the subphase were also performed to determine the maximum insertion pressure (MIP) of R and the association binding constant (Kb=ka/kd).The results revealed differences in the affinity of the resin for the different membrane systems, with KbPOPC﹥KbFL﹥KbSM. Moreover, MIP values showed the same tendency (POPC= 46 mN/m; FL= 43.3 mN/m y MS= 33.1 mN/m). Regarding the isotherms, it was observed that R did not significantly affect their stability and compressibility. Finally, R induced an increase in DPH, TMA-DPH fluorescence intensity, suggesting an increase in probe membrane partitioning. The decrease in the SM generalized polarization parameter of Laurdan values (from 0.63 to 0.52) in the presence of R, indicates higher dipolar relaxation and lower molecular order.In conclusion, our study provides evidence for the interaction of R with purified membranes and shows that lipid films used to mimic SM, which contain only lipid components, exhibit a different behavior when interacting with the compound especially when the kinetics of the binding behavior was analyzed.
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Luna, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
LI Reunión Anual de la Sociedad Argentina de Biofísica
Córdoba
Argentina
Sociedad Argentina de Biofísica
Materia
NATURAL MEMBRANES
LANGMUIR FILMES
CANNABIS SATIVA RESIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/282102
Acceder
id CONICETDig_841034614fd757d36242ec51d8a0ede1
oai_identifier_str oai:ri.conicet.gov.ar:11336/282102
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence AnalysisClop, Eduardo MatiasLuna, AgustinTurina, Anahi del ValleNATURAL MEMBRANESLANGMUIR FILMESCANNABIS SATIVA RESINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The proposed molecule with therapeutic effects in C. sativa oils, cannabidiol (CBD), interacts with the CB1 receptor and modulates its activity, although it has been proposed that its interaction is more complex and less direct than that of tetrahidrocannabinol (THC). CBD has been observed to interact with the serotonin 5-HT1A receptor, influence GABAergic signaling and modulate the activity of enzymes and transporters. Given that CBD is a lipophilic compound, the study of non-receptor mediated interaction with biological membranes could contribute to the understanding of its mechanism of action.This research focused on analyzing the effect of a CBD-rich resin (CBD=92%, THC= 4%) on membrane models, with different degrees of complexity, by means of Langmuir films (LF) and fluorescence spectroscopy. LF were built from purified membranes of bovine brain synaptosomes (SM), as well as their lipid fraction (LF) and pure POPC phospholipids. Compression isotherms surface pressure () vs area, in the presence and absence of the resin (R) were performed for the three model membranes. Penetration experiments of R from the subphase were also performed to determine the maximum insertion pressure (MIP) of R and the association binding constant (Kb=ka/kd).The results revealed differences in the affinity of the resin for the different membrane systems, with KbPOPC﹥KbFL﹥KbSM. Moreover, MIP values showed the same tendency (POPC= 46 mN/m; FL= 43.3 mN/m y MS= 33.1 mN/m). Regarding the isotherms, it was observed that R did not significantly affect their stability and compressibility. Finally, R induced an increase in DPH, TMA-DPH fluorescence intensity, suggesting an increase in probe membrane partitioning. The decrease in the SM generalized polarization parameter of Laurdan values (from 0.63 to 0.52) in the presence of R, indicates higher dipolar relaxation and lower molecular order.In conclusion, our study provides evidence for the interaction of R with purified membranes and shows that lipid films used to mimic SM, which contain only lipid components, exhibit a different behavior when interacting with the compound especially when the kinetics of the binding behavior was analyzed.Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Luna, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaLI Reunión Anual de la Sociedad Argentina de BiofísicaCórdobaArgentinaSociedad Argentina de BiofísicaSociedad Argentina de BiofísicaAcierno, Juan PabloCelej, Maria SoledadVazquez, Diego Sebastian2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/282102Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis; LI Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2023; 176-176978-987-48938-1-9CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/congreso-2023/#resumenesNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-05-13T10:56:47Zoai:ri.conicet.gov.ar:11336/282102instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-05-13 10:56:48.054CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
title Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
spellingShingle Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
Clop, Eduardo Matias
NATURAL MEMBRANES
LANGMUIR FILMES
CANNABIS SATIVA RESIN
title_short Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
title_full Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
title_fullStr Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
title_full_unstemmed Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
title_sort Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis
dc.creator.none.fl_str_mv Clop, Eduardo Matias
Luna, Agustin
Turina, Anahi del Valle
author Clop, Eduardo Matias
author_facet Clop, Eduardo Matias
Luna, Agustin
Turina, Anahi del Valle
author_role author
author2 Luna, Agustin
Turina, Anahi del Valle
author2_role author
author
dc.contributor.none.fl_str_mv Acierno, Juan Pablo
Celej, Maria Soledad
Vazquez, Diego Sebastian
dc.subject.none.fl_str_mv NATURAL MEMBRANES
LANGMUIR FILMES
CANNABIS SATIVA RESIN
topic NATURAL MEMBRANES
LANGMUIR FILMES
CANNABIS SATIVA RESIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The proposed molecule with therapeutic effects in C. sativa oils, cannabidiol (CBD), interacts with the CB1 receptor and modulates its activity, although it has been proposed that its interaction is more complex and less direct than that of tetrahidrocannabinol (THC). CBD has been observed to interact with the serotonin 5-HT1A receptor, influence GABAergic signaling and modulate the activity of enzymes and transporters. Given that CBD is a lipophilic compound, the study of non-receptor mediated interaction with biological membranes could contribute to the understanding of its mechanism of action.This research focused on analyzing the effect of a CBD-rich resin (CBD=92%, THC= 4%) on membrane models, with different degrees of complexity, by means of Langmuir films (LF) and fluorescence spectroscopy. LF were built from purified membranes of bovine brain synaptosomes (SM), as well as their lipid fraction (LF) and pure POPC phospholipids. Compression isotherms surface pressure () vs area, in the presence and absence of the resin (R) were performed for the three model membranes. Penetration experiments of R from the subphase were also performed to determine the maximum insertion pressure (MIP) of R and the association binding constant (Kb=ka/kd).The results revealed differences in the affinity of the resin for the different membrane systems, with KbPOPC﹥KbFL﹥KbSM. Moreover, MIP values showed the same tendency (POPC= 46 mN/m; FL= 43.3 mN/m y MS= 33.1 mN/m). Regarding the isotherms, it was observed that R did not significantly affect their stability and compressibility. Finally, R induced an increase in DPH, TMA-DPH fluorescence intensity, suggesting an increase in probe membrane partitioning. The decrease in the SM generalized polarization parameter of Laurdan values (from 0.63 to 0.52) in the presence of R, indicates higher dipolar relaxation and lower molecular order.In conclusion, our study provides evidence for the interaction of R with purified membranes and shows that lipid films used to mimic SM, which contain only lipid components, exhibit a different behavior when interacting with the compound especially when the kinetics of the binding behavior was analyzed.
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Luna, Agustin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Turina, Anahi del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
LI Reunión Anual de la Sociedad Argentina de Biofísica
Córdoba
Argentina
Sociedad Argentina de Biofísica
description The proposed molecule with therapeutic effects in C. sativa oils, cannabidiol (CBD), interacts with the CB1 receptor and modulates its activity, although it has been proposed that its interaction is more complex and less direct than that of tetrahidrocannabinol (THC). CBD has been observed to interact with the serotonin 5-HT1A receptor, influence GABAergic signaling and modulate the activity of enzymes and transporters. Given that CBD is a lipophilic compound, the study of non-receptor mediated interaction with biological membranes could contribute to the understanding of its mechanism of action.This research focused on analyzing the effect of a CBD-rich resin (CBD=92%, THC= 4%) on membrane models, with different degrees of complexity, by means of Langmuir films (LF) and fluorescence spectroscopy. LF were built from purified membranes of bovine brain synaptosomes (SM), as well as their lipid fraction (LF) and pure POPC phospholipids. Compression isotherms surface pressure () vs area, in the presence and absence of the resin (R) were performed for the three model membranes. Penetration experiments of R from the subphase were also performed to determine the maximum insertion pressure (MIP) of R and the association binding constant (Kb=ka/kd).The results revealed differences in the affinity of the resin for the different membrane systems, with KbPOPC﹥KbFL﹥KbSM. Moreover, MIP values showed the same tendency (POPC= 46 mN/m; FL= 43.3 mN/m y MS= 33.1 mN/m). Regarding the isotherms, it was observed that R did not significantly affect their stability and compressibility. Finally, R induced an increase in DPH, TMA-DPH fluorescence intensity, suggesting an increase in probe membrane partitioning. The decrease in the SM generalized polarization parameter of Laurdan values (from 0.63 to 0.52) in the presence of R, indicates higher dipolar relaxation and lower molecular order.In conclusion, our study provides evidence for the interaction of R with purified membranes and shows that lipid films used to mimic SM, which contain only lipid components, exhibit a different behavior when interacting with the compound especially when the kinetics of the binding behavior was analyzed.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/282102
Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis; LI Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2023; 176-176
978-987-48938-1-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/282102
identifier_str_mv Interactions of Cannabis sativa-derived resin with Natural Membranes: A Langmuir Films and Fluorescence Analysis; LI Reunión Anual de la Sociedad Argentina de Biofísica; Córdoba; Argentina; 2023; 176-176
978-987-48938-1-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/congreso-2023/#resumenes
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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